Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley +1 more source
Influence of Reflective Coating on Temperature Field and Temperature Effect of CRTS III Slab Ballastless Tracks on Bridges. [PDF]
Song L, Wu L, Cui C, Yu Z.
europepmc +1 more source
Tumour‐proximal deep serum proteomics identifies biomarkers associated with ovarian cancer
New biomarkers are needed for early ovarian cancer detection. Ovarian (tumour‐proximal) and peripheral vein serum from healthy women and patients with benign or malignant tumours underwent nanoparticle‐enriched deep proteomics. Ovarian serum yielded more protein identifications and revealed tumour‐reflective signatures.
Darren Lau +9 more
wiley +1 more source
Temperature Effect on Residual Magnetic Field of Atomic Gyroscope Magnetic Shielding System: A High-Precision Modeling Method. [PDF]
Chen Y +5 more
europepmc +1 more source
Temperature effect on polymerase fidelity. [PDF]
Xue Y, Braslavsky I, Quake SR.
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
The point spread function (PSF) inconsistency caused by temperature variation leads to artifacts in decoded images of a wavefront coding infrared imaging system.
Shi ZL(史泽林) +4 more
core
Comparative analysis of temperature effect on bandgap characteristics in 1D phononic crystals: Periodic versus quasiperiodic structures. [PDF]
Heravi FJ +9 more
europepmc +1 more source
Temperature effect on cable force of a special-shaped tied-arch bridge. [PDF]
Zhu L +7 more
europepmc +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source

