Results 31 to 40 of about 692,010 (217)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Spatio-Temporal Variation and Monsoon Effect on the Temperature Lapse Rate of a Subtropical Island

Terrestrial, Atmospheric and Oceanic Sciences, 2014
Temperature lapse rate (TLR) has been widely used in the prediction of mountain climate and vegetation and in many ecological models. The aims of this paper are to explore the spatio-temporal variations and monsoon effects on the TLR in the subtropical ...
Ching-An Chiu, Po-Hsiung Lin, Chih-Yung Tsai   +2 more
doaj   +1 more source

Effects of Agricultural Management Practices on the Temporal Variability of Soil Temperature under Different Crop Rotations in Bad Lauchstaedt-Germany

Agronomy, 2022
To investigate the effects of management practices on the dynamics of soil temperature, during 2014–2017, a field experiment was carried out in Bad Lauchstaedt, Germany.
Mahboube Jarrah, Sonia Mayel, Uwe Franko, Katrin Kuka   +3 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Effect of surface temperature on spreading rate of liquid droplet

MATEC Web of Conferences, 2014
Experimental investigation of spreading rate variation of liquid droplet under the conditions of its free-falling on a heated surface has been executed.
Batischeva Ksenia, Orlova Evgenija, Feoktistov Dmitriy   +2 more
doaj   +1 more source

Thermodynamic Characteristics of CH4 Adsorption on Shale at Different Temperatures

Geofluids, 2022
In order to clarify the effect of temperature on the isosteric heat of adsorption of CH4 onto shale, by using the isothermal adsorption data of shale in the temperature range of 300~473 K and the pressure range of 0.08~14 MPa, the isosteric heat of ...
Pei Xue, Quansheng Liang, Shiyan Hao, Jintao Yin, Chao Gao, Lixia Zhang, Qianping Zhao   +6 more
doaj   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

Interspecific variation in evaporative water loss and temperature response, but not metabolic rate, among hibernating bats

Scientific Reports, 2021
Hibernation is widespread among mammals in a variety of environmental contexts. However, few experimental studies consider interspecific comparisons, which may provide insight into general patterns of hibernation strategies. We studied 13 species of free-
Liam P. McGuire, Nathan W. Fuller, Yvonne A. Dzal, Catherine G. Haase, Brandon J. Klüg-Baerwald, Kirk A. Silas, Raina K. Plowright, Cori L. Lausen, Craig K. R. Willis, Sarah H. Olson   +9 more
doaj   +1 more source