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Orchid Micropropagation Using Temporary Immersion Systems: A Review
Temporary immersion systems (TIS) have been used for orchid micropropagation. The main advantage of TIS use for micropropagation is that the explant is periodically immersed in nutrient media, and then, the nutrient solution is drained, which allows the explant tissue to stay in air.Obdulia, Baltazar-Bernal +2 more
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Micropropagation Methods in Temporary Immersion Systems
Shoots from European chestnut and hybrids of European and Asian chestnuts can be efficiently proliferated in liquid medium by temporary immersion (TIS) using RITA® and Plantform™ bioreactors.
Cuenca, Beatriz +2 more
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Conclusions and Perspectives on Plant Micropropagation in Temporary Immersion Systems
In vitro propagation protocols that include temporary immersion systems are available for the most economically important plant species. However, these have not been established yet for multiple species. Having protocols validated by the scientific community guarantees the success of the mass production of commercial propagules.Marco A, Ramírez-Mosqueda +1 more
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Alstroemeria Micropropagation in a RITA® Temporary Immersion System
The use of temporary immersion systems (TIS) for plant micropropagation is an efficient technique for plant production, and we have applied it for the production of alstroemerias. This method involves the cultivation of explants such as rhizomes and axillary buds in a nutrient medium to stimulate shoot growth.Lilia Castro Pereira +4 more
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Use of Temporary Immersion Systems in the Establishment of Biofactories
Companies dedicated to the large-scale propagation of plant species are known as biofactories or agricultural biotechnology companies. Globally, there are a large number of biofactories (large-scale production) or plant tissue culture laboratories (small-scale production) in charge of supplying commercial propagules of plants of economic interest. Eachopenaire +2 more sources
Application of a temporary immersion system in mass propagation of Phalaenopsis
2005A temporary immersion system consisting of a series of five-litre twin glass vessels was successfully used for in vitro adventitious shoot multiplication and rooting of Phalaenopsis. Highest shoot multiplication rate of 25.4 was achieved after twelve weeks when eight immersions per day, of ten minutes each, were used. Fresh medium containing 0.5 mg l−1
Tino Hempfling, Walter Preil
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Types of Temporary Immersion Systems Used in Commercial Plant Micropropagation
Technological innovation in the design and manufacture of temporary immersion systems (TIS) has increased in the past decade. Innovations have involved the size, fitting, and replacement of components, as well as manufacturing materials. Air replacement by compressor has also been substituted by air replacement by preset tilting/rotation of culture ...Ivonne N, Bravo-Ruíz +4 more
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A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut
Plant Cell, Tissue and Organ Culture (PCTOC), 2015A protocol for culturing chestnut axillary shoots by temporary immersion in liquid medium was developed. The influence of type of explant, support material, bioreactor, and immersion was investigated for five artificial hybrids and five natural hybrids of Asian and European chestnut selected for resistance to ink disease. The type of explant influenced
N. Vidal, B. Blanco, B. Cuenca
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TEMPORARY IMMERSION SYSTEMS FOR EFFICIENT MASS PROPAGATION OF MEDICINAL AND AROMATIC PLANTS
Acta Horticulturae, 2015Plant production through in vitro propagation is nowadays a well-established industry. Micropropagation protocols are widely based on semi-solid nutrient media, despite their inherent limitations. Expensive gelling agents, large numbers of relatively small containers, limited nutrient diffusion, large culture areas, and extensive manual handling can ...
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Efficiency-cost, micropropagation, bioreactors, temporary immersion systems
Agro ProductividadObjective: to estimate the production price of strawberry “mother” plant of the CP-Jacona variety in both TIS and TS, and to compare these prices with the price of the imported “mother” plant. Design/Methodology/Approach: micropropagation methods have been used as an effective means for the mass production of pathogen-free plants, in small spaces and ...
Martha Alejandra Gomez Cruz +4 more
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