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Micropropagation of Mokara Orchid by Temporary Immersion System Technique
International Journal of Research and Innovation in Applied Science, 2022Based young leaves of Mokara Leuen Berger Gold were used as cultured materials. Callus was initiated and increase biomass on medium of semisolid and liquid cultures: MS medium supplemented with CW (30%), sucrose (30 g/l), 2.4D (1 mg/l). Callus was used as materials for initiation and biomass propagation on medium: (1) MS medium supplemented with NAA (1
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Plant Micropropagation and Temporary Immersion Systems
Temporary immersion systems (TIS) have been widely recognized as a promising technology for micropropagation of various plant species. The TIS provides a suitable environment for culture and allows intermittent contact of the explant with the culture medium at different immersion frequencies and aeration of the culture in each cycle.Hugo A, Méndez-Hernández +1 more
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Production of yam microtubers using a temporary immersion system
Plant Cell, Tissue and Organ Culture, 2005Yam clones ‚Pacala Duclos’ and ‚Belep’ in temporary immersion system culture showed favourable results on shoot growth stage and in the development of microtubers in comparison with solid culture media. Cultures in temporary immersion systems in both clones obtained a higher microtuber number per plant, with greater fresh weight and diameter in ...
Manuel Cabrera Jova +8 more
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Temporary Immersion Systems in Plant Micropropagation
Temporary immersion systems (TIS) are technological tools that support plant micropropagation. Given their high efficiency in the in vitro propagation of shoots, a current goal is to update the protocols addressing micropropagation in semisolid culture systems to protocols involving TIS.Marco A, Ramírez-Mosqueda +1 more
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Influence of Vitrofural® on sugarcane micropropagation using temporary immersion system
Plant Cell, Tissue and Organ Culture (PCTOC), 2020Sugarcane cultivation is of great economic and social importance worldwide. In vitro cloning using temporary immersion systems is an option to produce vitroplants of high genetic and phytosanitary quality to establish stock seedbeds. However, the sterilization of culture media through autoclaving increases production costs and limits the efficiency of ...
Alina Martínez Rivero +7 more
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Orchid Micropropagation Using Temporary Immersion Systems: A Review
Temporary immersion systems (TIS) have been used for orchid micropropagation. The main advantage of TIS use for micropropagation is that the explant is periodically immersed in nutrient media, and then, the nutrient solution is drained, which allows the explant tissue to stay in air.Obdulia, Baltazar-Bernal +2 more
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Efficiency-cost, micropropagation, bioreactors, temporary immersion systems
Agro ProductividadObjective: to estimate the production price of strawberry “mother” plant of the CP-Jacona variety in both TIS and TS, and to compare these prices with the price of the imported “mother” plant. Design/Methodology/Approach: micropropagation methods have been used as an effective means for the mass production of pathogen-free plants, in small spaces and ...
Martha Alejandra Gomez Cruz +4 more
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BioMINT: A Temporary Immersion System for Agave Micropropagation
Agaves are cultivated in Mexico as a source of industrial products such as fibers, nutritional supplements, and alcoholic beverages. Due to the demand for plant material, its long-life cycle, and the need to avoid predation on its natural populations, in vitro micropropagation represents a good option for agaves.Kelly M, Monja-Mio +4 more
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Alstroemeria Micropropagation in a RITA® Temporary Immersion System
The use of temporary immersion systems (TIS) for plant micropropagation is an efficient technique for plant production, and we have applied it for the production of alstroemerias. This method involves the cultivation of explants such as rhizomes and axillary buds in a nutrient medium to stimulate shoot growth.Lilia Castro Pereira +4 more
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Proliferation of Axillary Shoots of Chestnut in Temporary Immersion Systems
Shoots from European chestnut and hybrids of European and Asian chestnuts can be efficiently proliferated in liquid medium by temporary immersion (TIS) using RITA® and Plantform™ bioreactors. The main challenges of applying TIS to these species were the lack of growth and hyperhydricity; problem solutions included the manipulation of the hormone ...Nieves, Vidal +2 more
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