Results 81 to 90 of about 725,409 (217)
Advanced Science, EarlyView.AI‑guided single‑cell transcriptomics identifies OGT as a key regulator enriched in rheumatoid arthritis fibroblast‑like synoviocytes (RA‐FLSs). Inflammatory stimuli activate the OGT–SAP130 axis, which modulates the H3K27ac/H3K27me3 balance and epigenetically silences BTG2, promoting aggressive transformation of RA‑FLSs.
Duoli Xie +14 more
wiley +1 more source
Flow cytometry in the analysis of cellular populations [PDF]
, 2012 Podeu consultar el llibre complet a: http://hdl.handle.net/2445/32166Flow cytometry has become a valuable tool in cell biology. By analyzing large number of cells individually using light-scatter and fluorescence measurements, this technique reveals both
Castaño Boldú, Esther +1 more
core +3 more sources
A zebrafish model for hearing loss and regeneration induced by blast wave
Journal of Bio-X Research, 2019 . Zebrafish have the potential to regrow injured organs and tissues, but their use as a model for hearing regeneration following blast injury has never been reported.
Jiping Wang +7 more
doaj +1 more source
Advanced Science, EarlyView.A pathology‐informed engineering strategy rescues reconstituted HDL from inflammatory dysfunction in sepsis. By integrating a ceria nanozyme‐celastrol coordination complex, the platform preserves HDL structure and endotoxin handling, sustains hierarchical intracellular delivery, and enhances immunoregulation across pathological models, translating ...
Han Zhou +12 more
wiley +1 more source
Advanced Science, EarlyView.A HER3‐targeted sonogenetic lipid nanoparticle platform is developed for triple‐negative breast cancer therapy. An AI‐designed miniprotein inhibits HER3 signaling, while TRPV4 and NFAT‐responsive IL‐15 plasmids enable ultrasound‐controlled calcium signaling and cytokine expression. The system induces Ca2+ overload, ROS generation, mitochondrial damage,
Junya Song +6 more
wiley +1 more source
BioTechniques, 2006 A simple enzymatic labeling procedure is described to determine spot quality in oligonucleotide microarrays. By using fluorescently labeled dideoxynucleotides or ribonucleotides as substrate for terminal deoxynucleotidyl transferase (TdT), a single ...
Cesar E. Guerra
doaj +1 more source
Applications of Terminal Deoxynucleotidyl Transferase Enzyme in Biotechnology [PDF]
The use of polymerase enzymes in biotechnology has allowed us to gain unprecedented control over the manipulation of DNA, opening up new and exciting applications in areas such as biosensing, polynucleotide synthesis, and DNA storage aptamer development ...
Potts, IG, Olorunniji, FJ, Ashley, Jon
core +1 more source
Animal Models and Experimental Medicine, EarlyView.We investigated the therapeutic potential and mechanism of chrysin in experimental diabetic retinal disease. Using a streptozotocin (STZ)‐induced diabetic mouse model and high‐glucose‐stimulated BV2 microglia, we revealed chrysin‐responsive pyroptosis‐associated signatures and identified GBP3 as a regulated node.
Qun Liu +9 more
wiley +1 more source
, 1989 Transcriptional regulation of expression of the terminal deoxynucleotidyl transferase gene in normal thymus and in differentiation arrested cells was demonstrated by analyzing steady-state levels of TdT RNA as well as the relative transcription rate of ...
Trangas, T., Coleman, M. S.
core
Functional characterization of human terminal deoxynucleotidyl transferase polymorphisms [PDF]
, 2013 Terminal deoxynucleotidyl transferase (TdT) contributes to antigen receptor diversity of B and T lymphocytes of the adaptive immune system. TdT is a DNA polymerase catalyzing template independent addition of nucleotides during the process of V(D)J ...
Troshchynsky, Anna
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