Intestinal microbiota is different in women with preterm birth: results from terminal restriction fragment length polymorphism analysis. [PDF]
PLoS ONE, 2014 Preterm birth is a leading cause of perinatal morbidity and mortality. Studies using a cultivation method or molecular identification have shown that bacterial vaginosis is one of the risk factors for preterm birth.
Arihiro Shiozaki +6 more
doaj +5 more sources
Terminal Restriction Fragment Length Polymorphism for the Identification of Spirorchiid Ova in Tissues from the Green Sea Turtle, Chelonia mydas. [PDF]
PLoS ONE, 2016 Blood flukes are among the most common disease causing pathogens infecting vertebrates, including humans and some of the world's most globally endangered fauna.
Phoebe A Chapman +6 more
doaj +5 more sources
Pros and cons of ion-torrent next generation sequencing versus terminal restriction fragment length polymorphism T-RFLP for studying the rumen bacterial community. [PDF]
PLoS ONE, 2014 The development of next generation sequencing has challenged the use of other molecular fingerprinting methods used to study microbial diversity. We analysed the bacterial diversity in the rumen of defaunated sheep following the introduction of different
Gabriel de la Fuente +5 more
doaj +5 more sources
16S rRNA terminal restriction fragment length polymorphism for the characterization of the nasopharyngeal microbiota. [PDF]
PLoS ONE, 2012 A novel non-culture based 16S rRNA Terminal Restriction Fragment Length Polymorphism (T-RFLP) method using the restriction enzymes Tsp509I and Hpy166II was developed for the characterization of the nasopharyngeal microbiota and validated using recently ...
Silvio D Brugger +5 more
doaj +8 more sources
Terminal restriction fragment length polymorphism is an "old school" reliable technique for swift microbial community screening in anaerobic digestion. [PDF]
Sci Rep, 2018 The microbial community in anaerobic digestion has been analysed through microbial fingerprinting techniques, such as terminal restriction fragment length polymorphism (TRFLP), for decades.
De Vrieze J +3 more
europepmc +9 more sources
Methodological Aspects of Multiplex Terminal Restriction Fragment Length Polymorphism-Technique to Describe the Genetic Diversity of Soil Bacteria, Archaea and Fungi [PDF]
Sensors, 2020 The molecular fingerprinting methods used to evaluate soil microbial diversity could also be used as effective biosensors for the purposes of monitoring ecological soil status.
Agata Gryta, Magdalena Frąc
doaj +3 more sources
Terminal Restriction Fragment Length Polymorphism Analysis of Soil Bacterial Communities under Different Vegetation Types in Subtropical Area. [PDF]
PLoS ONE, 2015 Soil microbes are active players in energy flow and material exchange of the forest ecosystems, but the research on the relationship between the microbial diversity and the vegetation types is less conducted, especially in the subtropical area of China ...
Zeyan Wu +5 more
doaj +3 more sources
Trends in bacterial and fungal communities in ant nests observed with Terminal-Restriction Fragment Length Polymorphism (T-RFLP) and Next Generation Sequencing (NGS) techniques-validity and compatibility in ecological studies. [PDF]
PeerJ, 2018 Microbes are ubiquitous and often occur in functionally and taxonomically complex communities. Unveiling these community dynamics is one of the main challenges of microbial research.
Lindström S +4 more
europepmc +3 more sources
Comparison of the gut microbiota composition between obese and non-obese individuals in a Japanese population, as analyzed by terminal restriction fragment length polymorphism and next-generation sequencing. [PDF]
BMC Gastroenterol, 2015 BackgroundObesity has become one of the most serious social problems in developed countries, including Japan. The relationship between the gut microbiota and obesity has recently attracted the attention of many researchers.
Kasai C +10 more
europepmc +2 more sources
TRiFLe, a program for in silico terminal restriction fragment length polymorphism analysis with user-defined sequence sets. [PDF]
Appl Environ Microbiol, 2008 Junier P, Junier T, Witzel KP.
europepmc +3 more sources