Technicalities and Glitches of Terminal Restriction Fragment Length Polymorphism (T-RFLP). [PDF]
Indian J Microbiol, 2014 Terminal restriction fragment length polymorphism (T-RFLP) is a rapid, robust, inexpensive and simple tool for microbial community profiling. Methods used for DNA extraction, PCR amplification and digestion of amplified products have a considerable impact on the results of T-RFLP.
Prakash O +4 more
europepmc +5 more sources
Improved dsrA-based terminal restriction fragment length polymorphism analysis of sulfate-reducing bacteria. [PDF]
Appl Environ Microbiol, 2010 ABSTRACT To better describe the community structure of sulfate-reducing bacteria in environmental systems, we compared several dissimilatory sulfite reductase ( dsr ) primer sets for terminal restriction fragment length polymorphism application.
Santillano D, Boetius A, Ramette A.
europepmc +7 more sources
Evaluation of terminal-restriction fragment length polymorphism analysis in contrasting marine environments [PDF]
FEMS microbiology ecology, 2008 Terminal-restriction fragment length polymorphism (T-RFLP) analysis is widely used in microbial ecology studies. In the present study, T-RFLP analysis of PCR products digested by five restriction enzymes (AluI, HaeIII, MspI, Sau3AI and TaqI) was applied for 20 samples from three contrasting coastal environments to assess the biases associated with the ...
Rui Zhang +2 more
semanticscholar +7 more sources
Hayati Journal of Biosciences, 2023 Hypertension is a severe public health problem due to its high prevalence worldwide. About 7.5 million deaths or 12.8% of all annual deaths worldwide occur due to high blood pressure.
Ririn Herni Putri +2 more
doaj +3 more sources
Terminal Restriction Fragment Length Polymorphism Data Analysis for Quantitative Comparison of Microbial Communities [PDF]
Applied and Environmental Microbiology, 2003 ABSTRACT Terminal restriction fragment length polymorphism (T-RFLP) is a culture-independent method of obtaining a genetic fingerprint of the composition of a microbial community. Comparisons of the utility of different methods of (i) including peaks, (ii) computing the difference (or distance) between profiles, and (iii) performing ...
Christopher B. Blackwood +3 more
semanticscholar +5 more sources
The first decade of terminal restriction fragment length polymorphism (T-RFLP) in microbial ecology
Acta Agriculturae Slovenica, 2006 Terminal restriction fragment length polymorphism (T-RFLP) was introduced to environmental microbiology only a decade ago but it soon became a molecular tool of choice, due to its high throughput and phylogenetic resolution.
Blaž STRES
doaj +4 more sources
REPK: an analytical web server to select restriction endonucleases for terminal restriction fragment length polymorphism analysis. [PDF]
Nucleic Acids Res, 2007 Terminal restriction fragment length polymorphism (T-RFLP) analysis is a widespread technique for rapidly fingerprinting microbial communities. Users of T-RFLP frequently overlook the resolving power of well-chosen restriction endonucleases and often fail to report how they chose their enzymes.
Collins RE, Rocap G.
europepmc +5 more sources
New threshold and confidence estimates for terminal restriction fragment length polymorphism analysis of complex bacterial communities. [PDF]
Appl Environ Microbiol, 2006 ABSTRACT Terminal restriction fragment length polymorphism (T-RFLP) analysis has the potential to be useful for comparisons of complex bacterial communities, especially to detect changes in community structure in response to different variables.
Osborne CA +3 more
europepmc +5 more sources
T-RFPred: a nucleotide sequence size prediction tool for microbial community description based on terminal-restriction fragment length polymorphism chromatograms [PDF]
BMC Microbiology, 2010 Background Terminal-Restriction Fragment Length Polymorphism (T-RFLP) is a technique used to analyze complex microbial communities. It allows for the quantification of unique or numerically dominant phylotypes in amplicon pools and it has been used ...
Casamayor Emilio O +4 more
doaj +2 more sources
Comparison of gut microbiota composition between laboratory‐bred marmosets (Callithrix jacchus) with chronic diarrhea and healthy animals using terminal restriction fragment length polymorphism analysis [PDF]
Microbiology and immunology, 2018 Chronic diarrhea in laboratory‐bred marmosets poses a serious health problem during experiments. Despite a growing demand for laboratory‐bred experimental marmosets, the mechanisms underlying the development of diarrhea and measures for its treatment and
Yuko Shigeno +5 more
openalex +2 more sources