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Serial amplification of tau filaments using Alzheimer's brain homogenates and C322A or C322S recombinant tau. [PDF]
Santambrogio A +9 more
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Association of <i>NOS</i> Gene Polymorphisms with Sepsis-Related Complications in Secondary Peritonitis. [PDF]
Rasic M +12 more
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Thyroid Hormone Dynamics and DIO2 Variants in Schizophrenia: Exploring Genetic Links to Neuroendocrine Imbalance. [PDF]
Akan G +8 more
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Association Between Lactoferrin Gene Polymorphism and Dental Caries in Saudi Children: A Case-Control Study. [PDF]
Helal NM, Bagher A.
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Terminal Restriction Fragment Length Polymorphism (T-RFLP) to Estimate Fungal Diversity
Methods to Study Litter Decomposition, 2020The identification of aquatic hyphomycetes associated with decomposing leaves in streams has been traditionally based on inducing sporulation, but this approach misses non-sporulating mycelia. Molecular methods based on analyzing nucleic acid sequences circumvent this problem.
L. Nikolcheva, F. Bärlocher
semanticscholar +2 more sources
Multiplex-terminal restriction fragment length polymorphism
Nature Protocols, 2006A novel method called "multiplex-terminal restriction fragment length polymorphism (M-TRFLP)" has been recently developed which can be used for simultaneous analysis of the community composition of two or more microbial taxa (up to four). This method can also be used for microbial diagnostic purposes. For M-TRFLP analysis, primers specific to different
Singh, Brajesh K. (R15253) +1 more
openaire +3 more sources
Oral Microbiology and Immunology, 2009
Background: The aim of the study was to assess the bacterial community structures associated with endodontic infections using terminal restriction fragment length polymorphism (T‐RFLP), and to investigate the correlation of whole community profiles with the manifestation of particular clinical features.Methods: Intraradicular samples were collected ...
D, Saito +4 more
openaire +2 more sources
Background: The aim of the study was to assess the bacterial community structures associated with endodontic infections using terminal restriction fragment length polymorphism (T‐RFLP), and to investigate the correlation of whole community profiles with the manifestation of particular clinical features.Methods: Intraradicular samples were collected ...
D, Saito +4 more
openaire +2 more sources
Environmental Microbiology, 2006
Summary Residual activity of polymerase chain reaction DNA polymerases in restriction analyses strongly affected genetic profiling based on terminal restriction fragment length polymorphisms. Artificial fragment sizes produced as a result of 5′‐overhang restriction site fill‐in and addition of a terminal A may bias genetic profiling ...
Martin, Hartmann +2 more
openaire +2 more sources
Summary Residual activity of polymerase chain reaction DNA polymerases in restriction analyses strongly affected genetic profiling based on terminal restriction fragment length polymorphisms. Artificial fragment sizes produced as a result of 5′‐overhang restriction site fill‐in and addition of a terminal A may bias genetic profiling ...
Martin, Hartmann +2 more
openaire +2 more sources
Mycorrhiza, 2007
Terminal restriction fragment length polymorphism (T-RFLP) is an increasingly widely used technique in mycorrhizal ecology. In this paper, we review the technique as it is used to identify species of mycorrhizal fungi and distinguish two different versions of the technique: peak-profile T-RFLP (the original version) and database T-RFLP.
Ian A. Dickie, Richard G. FitzJohn
semanticscholar +3 more sources
Terminal restriction fragment length polymorphism (T-RFLP) is an increasingly widely used technique in mycorrhizal ecology. In this paper, we review the technique as it is used to identify species of mycorrhizal fungi and distinguish two different versions of the technique: peak-profile T-RFLP (the original version) and database T-RFLP.
Ian A. Dickie, Richard G. FitzJohn
semanticscholar +3 more sources
Journal of Microbiological Methods, 2010
To establish molecular monitoring for the phytoplankton community in aquatic ecosystems, we analysed the terminal restriction fragment length polymorphism (T-RFLP) of small subunit ribosomal RNA gene (18S rDNA) sequences of nuclear genomes from the algal strains of culture collections and environmental samples of two freshwater reservoirs (Sangcheon ...
Sungbae, Joo +2 more
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To establish molecular monitoring for the phytoplankton community in aquatic ecosystems, we analysed the terminal restriction fragment length polymorphism (T-RFLP) of small subunit ribosomal RNA gene (18S rDNA) sequences of nuclear genomes from the algal strains of culture collections and environmental samples of two freshwater reservoirs (Sangcheon ...
Sungbae, Joo +2 more
openaire +2 more sources

