Terminal restriction fragment length polymorphism (T-RFLP) analysis: Characterizing the unseen. [PDF]
Indian J Microbiol, 2007 The golden era of microbiology during early 1900s was based on the isolation and characterization of purified single cultures. However, since the understanding of limitations of culturing methods to decipher the majority of the microbial diversity, microbiologists have been keen to assess the abundance and distribution of microbial diversity by ...
Pandey J, Sood SS, Jain RK.
europepmc +4 more sources
Fidelity of Select Restriction Endonucleases in Determining Microbial Diversity by Terminal-Restriction Fragment Length Polymorphism [PDF]
Applied and Environmental Microbiology, 2003 ABSTRACT An evaluation of 18 DNA restriction endonucleases for use in terminal-restriction fragment length polymorphism (T-RFLP) analysis was performed by using richness and density indices in conjunction with computer simulations for 4,603 bacterial small-subunit rRNA gene sequences.
J. Engebretson, C. Moyer
semanticscholar +3 more sources
Formation of Pseudo-Terminal Restriction Fragments, a PCR-Related Bias Affecting Terminal Restriction Fragment Length Polymorphism Analysis of Microbial Community Structure [PDF]
Applied and Environmental Microbiology, 2003 ABSTRACT Terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR-amplified genes is a widely used fingerprinting technique in molecular microbial ecology. In this study, we show that besides expected terminal restriction fragments (T-RFs), additional secondary T-RFs occur in T-RFLP analysis of amplicons from ...
M. Egert, M. Friedrich
semanticscholar +5 more sources
High-sensitivity stable-isotope probing by a quantitative terminal restriction fragment length polymorphism protocol. [PDF]
Appl Environ Microbiol, 2012 ABSTRACT Stable-isotope probing (SIP) has proved a valuable cultivation-independent tool for linking specific microbial populations to selected functions in various natural and engineered systems. However, application of SIP to microbial populations with relatively minor buoyant density increases, such as populations that utilize
Andeer P, Strand SE, Stahl DA.
europepmc +4 more sources
Terminal restriction fragment length polymorphism for identification of Cryptosporidium species in human feces. [PDF]
Appl Environ Microbiol, 2009 ABSTRACT Effective management of human cryptosporidiosis requires efficient methods for detection and identification of the species of Cryptosporidium isolates. Identification of isolates to the species level is not routine for diagnostic assessment of cryptosporidiosis, which leads to uncertainty about ...
Waldron LS +3 more
europepmc +4 more sources
Cellulosomics, a gene-centric approach to investigating the intraspecific diversity and adaptation of Ruminococcus flavefaciens within the rumen [PDF]
, 2011 Peer reviewedPublisher ...
Bayer, Edward A +13 more
core +16 more sources
Characterization of the archaeal community in a minerotrophic fen and terminal restriction fragment length polymorphism-directed isolation of a novel hydrogenotrophic methanogen. [PDF]
Appl Environ Microbiol, 2008 Cadillo-Quiroz H +3 more
europepmc +2 more sources
TRFMA: a web-based tool for terminal restriction fragment length polymorphism analysis based on molecular weight [PDF]
Bioinformatics, 2006 Abstract Summary: TRFMA provides a Web environment for analyzing T-RFLP results based on molecular weights of the fragments, rather than the numbers of nucleotides, to increase accuracy. The 16S rRNA data are saved as an XML file containing around 650 sequences (light version) and a MySQL database containing around 50 000 sequences (full
Yoshio Nakano +2 more
openalex +4 more sources