Determining a minimum detection threshold in terminal restriction fragment length polymorphism analysis [PDF]
Terminal restriction fragment length polymorphism (T-RFLP) analysis is a common technique used to characterize soil microbial diversity. The fidelity of this technique in accurately reporting diversity has not been thoroughly evaluated. Here we determine if rare fungal species can be reliably detected by T-RFLP analysis.
Courtney, Kevin C. +6 more
openaire +3 more sources
Terminal restriction fragment length polymorphism analysis of the diversity of fecal microbiota in patients with ulcerative colitis [PDF]
Akira Andoh +5 more
openalex +2 more sources
Changes in the ruminal fermentation and bacterial community structure by a sudden change to a high-concentrate diet in Korean domestic ruminants [PDF]
Objective To investigate changes in rumen fermentation characteristics and bacterial community by a sudden change to a high concentrate diet (HC) in Korean domestic ruminants.
Mingyung Lee +3 more
doaj +1 more source
Coupling of denaturing high-performance liquid chromatography and terminal restriction fragment length polymorphism with precise fragment sizing for microbial community profiling and characterization. [PDF]
Penny C +5 more
europepmc +2 more sources
A double color terminal restriction fragment length polymorphism analysis was applied for the diversity assessment of a complex bacterial community in a deciduous forest soil. Application of the second label was used to project a specific taxonomic group
J. Kopecký +2 more
doaj +1 more source
Deep sequencing identifies ethnicity-specific bacterial signatures in the oral microbiome. [PDF]
Oral infections have a strong ethnic predilection; suggesting that ethnicity is a critical determinant of oral microbial colonization. Dental plaque and saliva samples from 192 subjects belonging to four major ethnicities in the United States were ...
Matthew R Mason +4 more
doaj +1 more source
Depth-dependent differences in community structure of the human colonic microbiota in health.
ObjectiveThe aims of this study were to develop techniques for spatial microbial assessment in humans and to establish colonic luminal and mucosal spatial ecology, encompassing longitudinal and cross-sectional axes.DesignA microbiological protected ...
Aonghus Lavelle +12 more
doaj +1 more source
Deletion within the Src homology domain 3 of Bruton's tyrosine kinase resulting in X-linked agammaglobulinemia (XLA). [PDF]
The gene responsible for X-linked agammaglobulinemia (XLA) has been recently identified to code for a cytoplasmic tyrosine kinase (Bruton's agammaglobulinemia tyrosine kinase, BTK), required for normal B cell development. BTK, like many other cytoplasmic
Chen, SH +11 more
core +1 more source
Salinity Inhibition in Thermophilic Anaerobic Digestion of Organic Waste
Anaerobic digestion, despite its preferable use as a treatment for high organic matter polluted waste streams, is susceptible to inhibitors, salt included.
Gregor Drago Zupančič +5 more
doaj +1 more source
Stable isotope probing: Technical considerations when resolving ¹⁵N-labeled RNA in gradients [PDF]
RNA based stable isotope probing (SIP) facilitates the detection and identification of active members of microbial populations that are involved in the assimilation of an isotopically labeled compound.
Addison, Sarah Louise +2 more
core +2 more sources

