Results 1 to 10 of about 157,193 (321)

Lipofection of Non-integrative CRISPR/Cas9 Ribonucleoproteins in Male Germline Stem Cells: A Simple and Effective Knockout Tool for Germline Genome Engineering

Frontiers in Cell and Developmental Biology, 2022
Gene editing in male germline stem (GS) cells is a potent tool to study spermatogenesis and to create transgenic mice. Various engineered nucleases already demonstrated the ability to modify the genome of GS cells. However, current systems are limited by
Mariella Obermeier, Jim Vadolas, Jim Vadolas, Stefaan Verhulst, Ellen Goossens, Yoni Baert   +5 more
doaj   +1 more source

Revised manuscript R2, clean version are serum levels of 25-hydroxy vitamin D reduced following orchiectomy in testicular cancer patients?

Basic and Clinical Andrology, 2021
Résumé Contexte Le testicule représente un lieu où le précurseur de la vitamine D est converti en sa forme active. Il a été suggéré que la perte d’un testicule pouvait induire une réduction des taux sériques de vitamine D.
Klaus-Peter Dieckmann, Osama Andura, Uwe Pichlmeier, Klaus Martin Otte, Hendrik Isbarn, Christian Wülfing   +5 more
doaj   +1 more source

Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation

Frontiers in Physiology, 2021
Short-term germ cell survival and central tissue degeneration limit organoid cultures. Here, testicular organoids (TOs) were generated from two different mouse strains in 3D printed one-layer scaffolds (1LS) at the air-medium interface displaying tubule ...
Guillaume Richer, Robin M. Hobbs, Katherine L. Loveland, Katherine L. Loveland, Ellen Goossens, Yoni Baert   +5 more
doaj   +1 more source

Micro RNA in Semen/Urine from Non-Obstructive Azoospermia Patients as Biomarkers to Predict the Presence of Testicular Spermatozoa and Spermatogonia

Life, 2023
About half of testicular sperm extraction (TESE) procedures in men with non-obstructive azoospermia (NOA), including men with Klinefelter syndrome (KS), are unsuccessful. To avoid unnecessary invasive surgery, biomarkers for spermatozoa were studied.
Margo Willems, Céline Devriendt, Catharina Olsen, Ben Caljon, Toon Janssen, Inge Gies, Veerle Vloeberghs, Herman Tournaye, Dorien Van Saen, Ellen Goossens   +9 more
doaj   +1 more source

Transcriptomic differences between fibrotic and non-fibrotic testicular tissue reveal possible key players in Klinefelter syndrome-related testicular fibrosis

Scientific Reports, 2022
Klinefelter syndrome (KS; 47,XXY) affects 1–2 in 1000 males. Most men with KS suffer from an early germ cell loss and testicular fibrosis from puberty onwards. Mechanisms responsible for these processes remain unknown. Previous genomics studies on testis
Margo Willems, Catharina Olsen, Ben Caljon, Veerle Vloeberghs, Jean De Schepper, Herman Tournaye, Dorien Van Saen, Ellen Goossens   +7 more
doaj   +1 more source

Does co-transplantation of mesenchymal and spermatogonial stem cells improve reproductive efficiency and safety in mice?

Stem Cell Research & Therapy, 2019
Background Spermatogonial stem cell transplantation (SSCT) is a promising therapy in restoring the fertility of childhood cancer survivors. However, the low efficiency of SSCT is a significant concern.
Prashant Kadam, Elissavet Ntemou, Jaime Onofre, Dorien Van Saen, Ellen Goossens   +4 more
doaj   +1 more source

Co-transplantation of mesenchymal stem cells improves spermatogonial stem cell transplantation efficiency in mice

Stem Cell Research & Therapy, 2018
Background Spermatogonial stem cell transplantation (SSCT) could become a fertility restoration tool for childhood cancer survivors. However, since in mice, the colonization efficiency of transplanted spermatogonial stem cells (SSCs) is only 12%, the ...
Prashant Kadam, Elissavet Ntemou, Yoni Baert, Sven Van Laere, Dorien Van Saen, Ellen Goossens   +5 more
doaj   +1 more source

Spermatogonial stem cells as a source for regenerative medicine

Middle East Fertility Society Journal, 2012
Several researchers have reported the derivation of multipotent cells from both mouse and human spermatogonial stem cells. These spermatogonia-derived stem cells show similarities with embryonic stem cells both for phenotype and functionality, indicating
Liang Ning, Ellen Goossens, Mieke Geens, Dorien Van Saen, Herman Tournaye   +4 more
doaj   +1 more source

Comparative Transcriptome Analysis of Differentially Expressed Genes and Signaling Pathways between XY and YY Testis in Yellow Catfish. [PDF]

PLoS ONE, 2015
YY super-males have rarely been detected in nature and only been artificially created in some fish species including tilapia and yellow catfish (Pelteobagrusfulvidraco), which provides a promising model for testis development and spermatogenesis.
Junjie Wu, Shuting Xiong, Jing Jing, Xin Chen, Weimin Wang, Jian-Fang Gui, Jie Mei   +6 more
doaj   +1 more source

Y/X-Chromosome-Bearing Sperm Shows Elevated Ratio in the Left but Not the Right Testes in Healthy Mice

Life, 2021
The sex chromosomes play central roles in determining the sex of almost all of the multicellular organisms. It is well known that meiosis in mammalian spermatogenesis produces ~50% Y- and ~50% X-chromosome-bearing sperm, a 1:1 ratio. Here we first reveal
Chengqing Hu, Jiangcheng Shi, Yujing Chi, Jichun Yang, Qinghua Cui   +4 more
doaj   +1 more source