Results 31 to 40 of about 132,891 (291)

Technical Note: Assay of cell quantity in the fibroblast-populated collagen matrix with a tetrazolium reagent [PDF]

European Cells & Materials, 2006
Determination of cell quantity in three-dimensional culture systems, such as the fibroblast-populated collagen matrix, can be difficult secondary to the abundance of extracellular collagen.
M A Carlson
doaj  

Varietal and germinative characterization of Agave potatorum (Asparagaceae) seeds with different origins

Botan‪ical Sciences, 2018
Background: Agave potatorum reproduces mainly through seeds is widely collected for the mezcal production, and there is currently a marked decrease in their wild populations.
Yolanda Donají Ortiz-Hernández, Germán Fernando Gutiérrez-Hernández, Luis Jorge Corzo-Ríos, Elpidio García-Ramírez, Sabino Honorio Martínez-Tomás   +4 more
doaj   +1 more source

Synthesis of 3-(phenylazo)-1,2,4-triazoles by a nucleophilic reaction of primary amines with 5-chloro-2,3-diphenyltetrazolium salt via mesoionic 2,3-diphenyltetrazolium-5-aminides

Beilstein Journal of Organic Chemistry, 2009
The reactions of a 5-chloro-2,3-diphenyltetrazolium salt with amines have been examined. In the presence of an inorganic base such as NaHCO3, primary and secondary amines undergo a nucleophilic substitution to give the corresponding 5-aminotetrazolium ...
Shuki Araki, Satoshi Hirose, Yoshikazu Konishi, Masatoshi Nogura, Tsunehisa Hirashita   +4 more
doaj   +1 more source

In vitro germination of zygotic embryos of hybrid BRS Manicoré (E. guineensis X E. oleifera)

Anais da Academia Brasileira de Ciências
The interspecific oil palm hybrid BRS Manicoré (E. guineensis x E. oleifera) has superior agronomic characteristics. However, the germination rate is low (30%) and the process is slow when the seeds are sown in a conventional form.
KEILA A.P. BONETTI, MARGUERITE QUOIRIN, REGINA C. QUISEN, SUELEN C.S. LIMA   +3 more
doaj   +1 more source

TETRAZOLIUM AND INTERACTION OF TEMPERATURE AND LIGHT UNDER SEED GERMINATION IN Ormosia arborea (FABACEAE)

Revista de Agricultura Neotropical, 2021
The study aimed to define the best conditions to conduct germination tests for Ormosia arborea seeds and assess the viability of seeds using the tetrazolium test.
Aparecida Leonir da Silva, Halisson César Vinci Carlos, Rodrigo Cyrino Rivaben, Laércio Junio Silva, Denise Cunha Fernandes dos Santos Dias, Glaucia Almeida de Morais, Liana Baptista de Lima   +6 more
doaj   +1 more source

Sowing seasons and qualitative performance of soybean seeds

Pesquisa Agropecuária Tropical, 2015
Changes in the soybean sowing season directly reflect on the physiological potential of the seeds produced. This study aimed to identify sowing seasons that qualitatively improve the soybean seeds yield.
Elesandro Bornhofen, Giovani Benin, Danielle Galvan, Mariana Faber Flores   +3 more
doaj   +1 more source

Suitability of tetrazolium test for Tamarindus indica L. seeds

Semina: Ciências Agrárias, 2022
Seed quality is routinely assessed by direct tests, e.g, the germination test, or indirect tests like the tetrazolium test, which has shoown to be promising in the determine viability and vigor, allowing the diagnosis of the main problems that may affect
Luciana Nunes Cordeiro, Riselane de Lucena Alcântara Bruno, Lucas Kennedy Silva Lima, Albericio Pereira de Andrade, Karialane da Silva Belarmino, Edna Ursulino Alves   +5 more
doaj   +1 more source

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source

Dual PI3K/AKT and CDK4/6 inhibition reveals selective sensitivity in an SHH medulloblastoma stem cell model

Molecular Oncology, EarlyView.
Targeted therapy was evaluated in SHH medulloblastoma using neuroepithelial stem cell (NES) and tumor‐derived NES‐like (tNES) models in 2D monolayers and 3D spheroids. PI3K, AKT, and CDK4/6 inhibitors had minimal effects in NES but markedly reduced viability and growth and induced apoptosis in tNES cells, revealing distinct therapeutic vulnerabilities.
Monika Lukoseviciute, Madeleine Birgersson, Paolo Ceriani, Margareta Wilhelm, Ourania N Kostopoulou   +4 more
wiley   +1 more source