Results 51 to 60 of about 675,988 (246)

Quick Test with Tetrazolium Chloride on Coffee Seed Viability

The Journal of Agriculture of the University of Puerto Rico, 2021
The germinative capacity of coffee seeds may be evaluated within 2 days by using the following procedure: 1. The parchment is removed from a representative sample of coffee seeds and the seeds are soaked in water for an hour or more. Soaking for 24 hours is recommended for older and drier seeds. 2.
openaire   +2 more sources

A Method of 1D UVC Radiation Dose Measurement using a Novel Tablet Dosimeter

AUTEX Research Journal, 2020
In this work, a method for the measurement of one-dimensional (1D) UV radiation dose is described. It comprises a new tablet dosimeter that measures the dose using reflectance spectrophotometry. The tablet dosimeter elaborated is a solid structure with a
Sąsiadek Elżbieta, Kozicki Marek
doaj   +1 more source

Toksisitas Senyawa Tembaga(II)Klorida Dihidrat Terhadap Sel Kanker Payudara T74D Secara in vitro

Qanun Medika: Jurnal Kedokteran Fakultas Kedokteran Universitas Muhammadiyah Surabaya, 2018
Cancer is a normal cell transformation in the body becomes malignant due to the induction of carcinogens. Breast cancer is the second leading cause of death in Indonesia. The field of treatment with inorganic compounds has been widely developed and shows
Teguh Hari Sucipto, Harsasi Setyawati, Fahimah Martak   +2 more
doaj   +1 more source

Comparative in situ hybridization protocols in zebrafish

BioTechniques, 2022
In situ hybridization is a commonly used technique in molecular biology to assess the temporal and spatial expression of a given gene. As a long and labor-intensive protocol, double in situ hybridization, which detects two genes in series, is challenging
Krista Dunn, Apoorva Vashisht, Dena R Hammond-Weinberger   +2 more
doaj   +1 more source

Mechanism of Enzymatic Reduction of Triphenyl Tetrazolium Chloride.

Experimental Biology and Medicine, 1951
SummaryThe conditions of the reduction of TTC by cytoplasmic fractions of rat tissue homogenates were determined. Rat kidney extracts, upon addition of aminoacids, reduce TTC anaerobically. Glycolytic enzymes of liver and muscle reduce TTC only when supplemented by mitochondria.
openaire   +2 more sources

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

FEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan, Bethany C. Guerra, Selina Uranga, Jessica M. Cardin, Steven E. Riechman, Mariana Janini Gomes, James D. Fluckey   +6 more
wiley   +1 more source

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Fluorescent Nanodiamonds Based Theranostic Platform for pH‐Sensitive Drug Delivery and Quantum Sensing

Advanced Functional Materials, EarlyView.
A multifunctional nanodiamond platform enables pH‐triggered Diazoxide (DZX) delivery and quantum sensing of subcellular radical dynamics in triple‐negative breast cancer cells. Diamond relaxometry revealed reduced lysosomal radicals during DZX‐induced mitochondrial radical elevation, providing insights into redox modulation and organelle‐ resolved ...
Kaiqui Wu, Siyu Fan, Yue Zhang, Willem Woudstra, Thomas Mulder, Laurens Boscher Navarro, Jur van Dijken, Katja Loos, Romana Schirhagl   +8 more
wiley   +1 more source

Ultrahigh‐Yield, Multifunctional, and High‐Performance Organic Memory for Seamless In‐Sensor Computing Operation

Advanced Functional Materials, EarlyView.
Molecular engineering of a nonconjugated radical polymer enables a significant enhancement of the glass transition temperature. The amorphous nature and tunability of the polymer, arising from its nonconjugated backbone, facilitates the fabrication of organic memristive devices with an exceptionally high yield (>95%), as well as substantial ...
Daeun Kim, Jaehyoung Ko, Namju Kim, Quynh H. Nguyen, Hoyeon Lee, Joohwan Eo, Seongeon Jin, Ji Eon Kwon, Seung‐Yeol Jeon, Youngdo Jeong, Dongseok Kwon, Sung Gap Im, Sanghan Lee, Byung Chul Jang, Yongho Joo   +14 more
wiley   +1 more source

Bioinspired Bromination Enables Extensible, Strain‐Stiffening Resilin Peptide Scaffolds with Tunable Degradation

Advanced Functional Materials, EarlyView.
Bioinspired bromination of a resilin‐derived peptide enables the fabrication of electrospun nanofibrous scaffolds that uniquely combine strain‐stiffening elasticity, proteolytic stability, and antioxidant functionality. These brominated peptide–gelatin hybrids mimic the extensibility of natural elastomers, demonstrating tunable mechanical resilience ...
Elisa Marelli, Lorenzo Sori, Chiara Russo, Claudia Pigliacelli, Lorenza Draghi, Monica Soncini, Pierangelo Metrangolo   +6 more
wiley   +1 more source