Results 151 to 160 of about 1,593,480 (263)

Simplified Laboratory Cultivation of <i>Thiobacillus denitrificans</i> and <i>Sulfurimonas denitrificans</i> with Practical Guidelines for Laboratories Without Advanced Anaerobic Equipment. [PDF]

Microorganisms
Kalebic B, Prgic Z, Marsavelski A, Ivankovic T.   +3 more
europepmc   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Optimizing submerged cultivation of Bacillus velezensis for endospore production and formulation. [PDF]

Braz J Microbiol
Gilio LA, Mascarin GM, Bettiol W.
europepmc   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Compositional Profiling of Carbonaceous Deposits From Failed Automotive Spark Plugs by Pyrolysis-Gas Chromatography Mass Spectrometry. [PDF]

Rapid Commun Mass Spectrom
Li Z, Cheng J, Zheng T, Liu B, Chen P.
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Modeling growth of <i>Lactobacillus helveticus</i> and <i>Kluyveromyces marxianus</i> isolated from fermented camel milk (shubat). [PDF]

Front Microbiol
Ospanova A, Khalbayev A, Akhmetsadykova S, Begdildayeva N, Bilal Z, Akhatzhanova A, Vinceković M, Kondybayev A.   +7 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Coconut Oil as Lipid-Based 3D-Printable Sacrificial Material for the Facile Biofabrication of Precise Perfusable Microchannels and Freeform Hydrogel Structures. [PDF]

Small Sci
Mansi S, Halbherr S, Möller F, Ernst AL, Bauer S, Kuznetsova Z, Karagiannakidou Samsarelou M, Rickert CA, Heinrich D, Zhong Z, Rojas-González DM, Lieleg O, Mela P.   +12 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source