Results 31 to 40 of about 1,593,480 (263)
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Introduction. P. aeruginosa and A. baumannii are important subjects of research into biofilm formation processes under various conditions. The formation of the biofilm phenotype is regulated by quorum-sensing signals under the influence of a complex of ...
Valentin P. Kovalchuk +6 more
doaj +1 more source
The work aims to determine the undetermined temperature distribution of the medium and the pipeline wall using the finite difference method. Time courses of the temperature of the flowing medium and pipeline walls caused by a step change in temperature ...
Sobota Tomasz +2 more
doaj +1 more source
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura +2 more
wiley +1 more source
Measuring the temperature of the intergalactic medium
Numerical simulations indicate that the smooth, photoionized intergalactic medium (IGM) responsible for the low column density Lyman-alpha forest follows a well defined temperature-density relation. We show that such an equation of state results in a cutoff in the distribution of line widths (b-parameters) as a function of column density (N) for the ...
Schaye, J. +3 more
openaire +3 more sources
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
Analysis of fungal community structure of medium-high temperature Daqu in Hetao area [PDF]
The fungal flora diversity and community structure of the medium-high temperature Daqu in Hetao area of Bayannur, Inner Mongolia was analyzed by Illumina MiSeq high-throughput sequencing technology, and compared with the fungal flora of medium-high ...
LI Na, ZHANG Haibo, YE Mingbo, ZHANG Min, LI Qing, GUO Zhuang, WANG Yurong
doaj +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
Air and root medium temperature and the rooting of chrysanthemum and rose cuttings
Cuttings of the chrysanthemum cultivar 'Horim' and rose cultivar 'Garnette' were rooted at 21°C, 24°C, 27°C root temperature in a factorial combination with 15°C, 18°C and 21°C air temperature. In each of the air and root temperatures, rose cuttings from
Edward Borowski, Paul Hagen, Hoar Moe
doaj +1 more source
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai +4 more
wiley +1 more source

