Results 41 to 50 of about 186,655,186 (256)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Analysis of fungal community structure of medium-high temperature Daqu in Hetao area [PDF]

Zhongguo niangzao
The fungal flora diversity and community structure of the medium-high temperature Daqu in Hetao area of Bayannur, Inner Mongolia was analyzed by Illumina MiSeq high-throughput sequencing technology, and compared with the fungal flora of medium-high ...
LI Na, ZHANG Haibo, YE Mingbo, ZHANG Min, LI Qing, GUO Zhuang, WANG Yurong
doaj   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Air and root medium temperature and the rooting of chrysanthemum and rose cuttings

Acta Agrobotanica, 2013
Cuttings of the chrysanthemum cultivar 'Horim' and rose cultivar 'Garnette' were rooted at 21°C, 24°C, 27°C root temperature in a factorial combination with 15°C, 18°C and 21°C air temperature. In each of the air and root temperatures, rose cuttings from
Edward Borowski, Paul Hagen, Hoar Moe
doaj   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

The Temperature Structure of the Warm-Hot Intergalactic Medium

, 2019
We study the temperature structure of the intergalactic medium (IGM) using a large cosmological N-body/ smoothed particle hydrodynamics simulation.
Furlanetto, Steven R., Yoshida, Naoki, Hernquist, Lars   +2 more
core   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

MEASURING TEMPERATURE OF THE MEDIUM OF SHIP COMPRESSOR PIPING USING TEMPERATURE OF THE OUTER WALL

, 2018
To determine the temperature of the medium in the ship's piping systems there are used readings of contact thermometers, pyrometers and thermal scans which measure the temperature of the pipeline rather accurately.
Sergey Anatolyevich Putilin, Alexander Evgenyevich Semenov   +1 more
core   +1 more source

Effective medium theory for the low-temperature heat capacity of a metasolid plate

Communications Materials, 2023
Modeling artificial nanostructures in terms of effective materials parameters is important for gaining physical insight into their behavior and facilitating their optimization.
Tuomas A. Puurtinen, Ilari J. Maasilta
doaj   +1 more source