Results 91 to 100 of about 27,828,593 (362)
Diffusion‐based size determination of solute particles: a method adapted for postsynaptic proteins
FEBS Open Bio, EarlyView.We present a diffusion‐based approach for measuring the size of macromolecules and their complexes, and demonstrate its use on postsynaptic proteins. The method requires fluorescein‐labelled protein samples, a microfluidic device that maintains laminar flow for said samples, a microscope recording the emitted fluorescent signals, and an analytic ...
András László Szabó +7 more
wiley +1 more source
Utilizing educational theoretical models to support effective physical education pedagogy
Cogent Education, 2015 Physical education (PE) pedagogy has traditionally been viewed as drill-style teaching. Whilst this traditional pedagogical approach provides exposure to various skills, used within a school-based PE and sporting context, it does not demonstrate a ...
Wayne Usher +2 more
doaj +1 more source
A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms
FEBS Open Bio, EarlyView.We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley +1 more source
Influence of Reynolds number on theoretical models for trailing vortices [PDF]
, 2015 We conduct direct numerical simulations for a NACA0012 airfoil at Reynolds numbers (Re) ranging from 300 to 7000 to determine the wake behavior behind this wing profile. We characterize the structure of the wing-tip vortex, finding a reasonable agreement
Del-Pino-Peñas, Carlos Manuel +4 more
core
Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Financial correlations at ultra-high frequency: theoretical models and empirical estimation
, 2011 A detailed analysis of correlation between stock returns at high frequency is compared with simple models of random walks. We focus in particular on the dependence of correlations on time scales - the so-called Epps effect.
A. Lo +24 more
core +2 more sources
Quantifying Transmission of Clostridium difficile within and outside Healthcare Settings
Emerging Infectious Diseases, 2016 To quantify the effect of hospital and community-based transmission and control measures on Clostridium difficile infection (CDI), we constructed a transmission model within and between hospital, community, and long-term care-facility settings.
David P. Durham +4 more
doaj +1 more source
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Theoretical models of synaptic short term plasticity
Frontiers in Computational Neuroscience, 2013 Received: 14 October 2013; accepted: 15 October 2013; published online: 30 October 2013. Citation: Hennig MH (2013) Theoretical models of synaptic short term plasticity. Front. Comput. Neurosci. 7:154.
Matthias H Hennig
semanticscholar +1 more source
, 2013 This chapter summarizes several approaches combining theory, simulation and experiment that aim for a better understanding of phenomena in lipid bilayers and membrane protein systems, covering topics such as lipid rafts, membrane mediated interactions ...
Deserno, Markus +4 more
core +1 more source