Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Mitigating risks of students use of study drugs through understanding motivations for use and applying harm reduction theory: a literature review. [PDF]
Abelman DD.
europepmc +1 more source
Acute caffeine treatment protects the developing retina from ischemia‐induced cell death
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento +6 more
wiley +1 more source
Conceptualization, Contexts, and Measurement of Nursing Theoretical Literacy: Protocol for a Scoping Review. [PDF]
Ge J +7 more
europepmc +1 more source
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin +3 more
wiley +1 more source
Challenges, Strategies, and Explanatory Mechanisms in Clinical Skills Remediation Programs in Undergraduate and Postgraduate Medical Education in Low- and Middle-Income Countries: Realist Review Protocol. [PDF]
Utama MR +9 more
europepmc +1 more source
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin +12 more
wiley +1 more source
Updates of the Status Quo of Situation-Specific Theories (2015-2025). [PDF]
Kim D, Im EO.
europepmc +1 more source
Sea of Literatures. Towards a Theory of Mediterranean Literature (Book Review)
Βιβλιοκρισία στο Sea of Literatures. Towards a Theory of Mediterranean Literature. Alpe Adria e dintorni, itinerari mediterranei (Series), των Angela Fabris, Albert Göschl, Steffen SchneiderΑ review of Sea of Literatures.
Petsa, Vasiliki
core +1 more source

