Results 61 to 70 of about 151,392 (262)

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Interplay Between Wear and Thermal Expansion in 6082 Aluminum: A Simulation and Experimental Study

open access: yesApplied Sciences
This study investigates how wear and thermal expansion interact in 6082 aluminum, utilizing a pin-on-disc tribometer and finite element analysis under diverse mechanical conditions. The findings show that thermal expansion reduces contact area by forming
Yang Tian   +3 more
doaj   +1 more source

Adjustable Thermal Expansion Properties in Zr2MoP2O12/ZrO2 Ceramic Composites

open access: yesFrontiers in Chemistry, 2018
Zr2MoP2O12/ZrO2 composites were successfully synthesized by the solid state method in attempt to fabricate the near-zero thermal expansion ceramics. The phase composition, micromorphology and thermal expansion behavior of the Zr2MoP2O12/ZrO2 composites ...
Hongfei Liu   +7 more
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

open access: yesFEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain   +11 more
wiley   +1 more source

On-the-fly thermal expansion for Monte Carlo multi-physics reactor simulations

open access: yesFrontiers in Nuclear Engineering
In this study, we present on-the-fly thermal expansion methodology for direct Monte Carlo coupled multi-physics reactor simulations. This approach allows the problem geometry to be thermally expanded on-the-fly during particle tracking using local ...
Muhammad Imron   +2 more
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

open access: yesFEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey   +4 more
wiley   +1 more source

Editorial: Recent Advances in Low-Positive, Zero, and Negative Thermal Expansion Materials: Fundamentals and Applications

open access: yesFrontiers in Materials, 2022
Bojan A. Marinkovic   +3 more
doaj   +1 more source

Aluminium-Silicon Lightweight Thermal Management Alloys with Controlled Thermal Expansion

open access: yesCrystals
With the ever-growing emphasis on global decarbonization and rapid increases in the power densities of electronics equipment in recent years, new methods and lightweight materials have been developed to manage heat load as well as interfacial stresses ...
Peter Lewis   +6 more
doaj   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

open access: yesFEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav   +4 more
wiley   +1 more source

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