Results 181 to 190 of about 665 (203)

Fluorescence-based thermal shift assays.

Current opinion in drug discovery & development, 2010
Thermal shift assays are one of the label-free technologies gaining popularity in drug discovery and academic research. This review summarizes the various detection formats used in these assays, and provides an in-depth presentation of fluorescence-based thermal shift assays.
Rumin, Zhang, Frederick, Monsma
openaire   +1 more source

Screening Chemoreceptor–Ligand Interactions by High-Throughput Thermal-Shift Assays

2018
Identifying the ligands sensed by chemoreceptors remains challenging, in part because current screening methods are low-throughput, costly, and/or time-consuming. In contrast, fluorescence thermal shift (FTS) assays provide a fast and inexpensive approach to chemoreceptor-ligand screening.
Maximilian K G, Ehrhardt, Suzanne L, Warring, Monica L, Gerth   +2 more
openaire   +2 more sources

Thermal shift assay for protein–ligand dissociation constant determination

TrAC - Trends in Analytical Chemistry
Asta Zubrienė, Vytautas Petrauskas, Egidijus Kazlauskas   +2 more
exaly   +2 more sources

Label-Free Target Identification and Confirmation Using Thermal Stability Shift Assays

2020
Target identification presents one of the biggest challenges to chemical genomic approaches. In recent years, several methods have been applied for target identification and validation in plant cells. Here, we describe a label-free method based on the thermodynamic stabilization of a protein by interaction with a small-molecule ligand.
Cecilia, Rodriguez-Furlan, Glenn R, Hicks   +1 more
openaire   +2 more sources

Inhibitor Binding to Carbonic Anhydrases by Fluorescent Thermal Shift Assay

2019
In this chapter we describe the model that quantifies interactions between proteins and ligands applying the thermal shift assay. When combined with fluorescence-based measurements of protein thermal unfolding, this model forms the basis of the fluorescent thermal shift assay—a widely applicable and cost-effective technique to quantify ligand affinity ...
Vytautas Petrauskas, Asta Zubrienė, Matthew J. Todd, Daumantas Matulis   +3 more
openaire   +1 more source

Thermal shift assays in drug discovery – Troubleshooting from biochemical to cellular applications

Journal of Pharmacological and Toxicological Methods
Thermal shift assays (TSAs) have become valuable tools within drug discovery and development pipelines. These techniques detect direct physical interactions between small, drug-like molecules and their target proteins in biochemical and cellular settings.
Danielle, Hanke, Jennifer I, Brown, Brent D G, Page   +2 more
openaire   +2 more sources

Applications of the Cellular Thermal Shift Assay to Drug Discovery in Natural Products: A Review

International Journal of Molecular Sciences
Natural products play a crucial role in drug discovery because of their structural diversity and biological activity. However, identifying their molecular targets remains a challenge. Traditional target identification approaches such as affinity-based protein profiling and activity-based protein profiling are limited by the need for chemical ...
exaly   +3 more sources

Fluorescent Thermal Shift Assays for Identifying Small Molecule Ligands

2017
Fluorescent thermal shift assay (FTSA; also known as ThermoFluor, differential scanning fluorimetry (DSF), thermal shift assay (TSA) and temperature-dependent fluorescence (TdF)) is a high-throughput, simple and inexpensive screening method to identify low-molecular weight ligands that bind to and stabilise purified protein.
openaire   +1 more source

Identification of the binding proteins of organic acid metabolites by matrix thermal shift assay

Chinese Journal of Chromatography
Organic acid metabolites exhibit acidic properties. These metabolites serve as intermediates in major carbon metabolic pathways and are involved in several biochemical pathways, including the tricarboxylic acid (TCA) cycle and glycolysis. They also regulate cellular activity and play crucial roles in epigenetics, tumorigenesis, and cellular signal ...
Ke-Jia, Li, Yu-Ying, Ye, Xiao-Lei, Zhang, Jia-Hua, Zhou, Ya-Nan, Li, Ming-Liang, Ye   +5 more
openaire   +2 more sources

Rapid Evaluation of Small Molecule Cellular Target Engagement with a Luminescent Thermal Shift Assay

ACS Medicinal Chemistry Letters, 2021
Jonathan Mortison, Simon Bushell, Ivan Cornella-Taracido   +2 more
exaly