Application of temperature-responsive HIS-tag fluorophores to differential scanning fluorimetry screening of small molecule libraries [PDF]
Frontiers in Pharmacology, 2022 Differential scanning fluorimetry is a rapid and economical biophysical technique used to monitor perturbations to protein structure during a thermal gradient, most often by detecting protein unfolding events through an environment-sensitive fluorophore.
Michael H. Ronzetti +8 more
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Nano Differential Scanning Fluorimetry as a Rapid Stability Assessment Tool in the Nanoformulation of Proteins [PDF]
Pharmaceutics, 2023 The development and production of innovative protein-based therapeutics is a complex and challenging avenue. External conditions such as buffers, solvents, pH, salts, polymers, surfactants, and nanoparticles may affect the stability and integrity of ...
Sofia Lisina +5 more
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Combining nano-differential scanning fluorimetry and microscale thermophoresis to investigate VDAC1 interaction with small molecules [PDF]
Journal of Enzyme Inhibition and Medicinal Chemistry, 2023 The mitochondrial voltage-dependent anion channel 1 (VDAC1) plays a central role in metabolism and apoptosis, which makes it a promising therapeutic target. Nevertheless, molecular mechanisms governing VDAC1 functioning remain unclear.
Hubert Gorny +5 more
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Screening of benzenesulfonamide in combination with chemically diverse fragments against carbonic anhydrase by differential scanning fluorimetry [PDF]
Journal of Enzyme Inhibition and Medicinal Chemistry, 2020 The differential scanning fluorimetry (DSF) screening of 5.692 fragments in combination with benzenesulfonamide (BSA) against bovine carbonic anhydrase (bCA) delivered >100 hits that either caused, on their own, a significant thermal shift (ΔTm, °C) in ...
Mikhail Krasavin +6 more
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Assessment of Thermal Stability of Mutant p53 Proteins via Differential Scanning Fluorimetry [PDF]
Life, 2022 The p53 protein is a transcription factor that preserves the integrity of the genome. The TP53 gene has inactivating mutations in about 50% of all human cancers.
Raniya Khadiullina +5 more
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Nano Differential Scanning Fluorimetry-Based Thermal Stability Screening and Optimal Buffer Selection for Immunoglobulin G [PDF]
Pharmaceuticals, 2021 Nano differential scanning fluorimetry (nanoDSF) is a high-throughput protein stability screening technique that simultaneously monitors protein unfolding and aggregation properties.
Soo Hyun Kim +3 more
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Application of differential scanning fluorimetry to X-ray protein crystallography [PDF]
IUCrJX-ray crystallography remains a powerful technique for determining high-resolution protein structures; however, obtaining high-quality crystals is a significant bottleneck.
Yukari Sato, Toshiya Senda
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Protocol for evaluating protein-polyfluoroalkyl substances in vitro using differential scanning fluorimetry [PDF]
STAR ProtocolsSummary: Per- and polyfluoroalkyl substances (PFAS) are ubiquitous synthetic chemicals that threaten public health, and serum albumin binding of PFAS represents one major variable influencing PFAS toxicokinetics.
Hannah M. Starnes, Scott M. Belcher
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Measuring the Thermal Unfolding of Lysozyme: A Critical Comparison of Differential Scanning Fluorimetry and Differential Scanning Calorimetry [PDF]
ChemistryOpenThe thermal unfolding of lysozyme in aqueous solution has been analyzed by (nano) differential scanning fluorimetry (nanoDSF) and differential scanning calorimetry (DSC).
Weronika Malicka +9 more
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Simple and High-Throughput Quantification of Mono- and Bivalent Foot-and-Mouth Disease Virus Vaccine Antigens by Differential Scanning Fluorimetry [PDF]
VaccinesBackground/Objectives: An accurate quantification of the effective antigens from different serotypes is essential for the quality control of multivalent vaccines, but it remains challenging.
Yanli Yang +8 more
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