Results 11 to 20 of about 6,190 (152)

Differential scanning fluorimetry to assess PFAS binding to bovine serum albumin protein [PDF]

Scientific Reports
The rapid screening of protein binding affinity for poly- and perfluoroalkyl substances (PFAS) benefits risk assessment and fate and transport modelling. PFAS are known to bioaccumulate in livestock through contaminated food and water.
Jessica Alesio, Geoffrey D. Bothun
doaj   +4 more sources

Intrinsic Differential Scanning Fluorimetry for Protein Stability Assessment in Microwell Plates [PDF]

Molecular Pharmaceutics
Intrinsic differential scanning fluorimetry (DSF) is essential for analyzing protein thermal stability. Until now, intrinsic DSF was characterized by medium throughput and high consumable costs. Here, we present a microplate-based intrinsic DSF approach that enables the measurement of up to 384 samples in parallel by consuming only 10 μL per sample. We
Hristo L Svilenov, Kevin Braeckmans, Stefaan C De Smedt   +2 more
exaly   +5 more sources

Aurora 2.0: A fluorogenic dye library for expanding the capability of protein-adaptive differential scanning fluorimetry (paDSF) [PDF]

SLAS Discovery
Differential Scanning Fluorimetry (DSF) is a biophysical assay that is used to estimate protein stability in vitro. In a DSF experiment, the increased fluorescence of a solvatochromatic dye, such as Sypro Orange, is used to detect the unfolding of a ...
Annemarie F. Charvat, Kayleigh Mason-Chalmers, Aneta Grabinska-Rogala, Shloka Shivakumar, Zachary Gale-Day, Taiasean Wu, Zoe Millbern, Jonathan B. Grimm, Emma C. Carroll, K․ Peter R․ Nilsson, Luke D. Lavis, Nelson R. Vinueza, Jason E. Gestwicki   +12 more
doaj   +2 more sources

Use of differential scanning fluorimetry to optimize the purification and crystallization of PLP-dependent enzymes [PDF]

Acta Crystallographica Section F: Structural Biology Communications, 2012
Differential scanning fluorimetry (DSF) is a practical and accessible technique that allows the assessment of multiphasic unfolding behavior resulting from subsaturating binding of ligands. Multiphasic unfolding is indicative of a heterogenous protein solution, which frequently interferes with crystallization and complicates functional characterization
Barry C Finzel
exaly   +5 more sources

Further validation of strecker-type α-aminonitriles as a new class of potent human carbonic anhydrase II inhibitors: hit expansion within the public domain using differential scanning fluorimetry leads to chemotype refinement [PDF]

Journal of Enzyme Inhibition and Medicinal Chemistry, 2020
Testing of an expanded, 800-compound set of analogues of the earlier described Strecker-type α-aminonitriles (selected from publicly available Enamine Ltd.
Mikhail Krasavin, Stanislav Kalinin, Sergey Zozulya, Anastasia Griniukova, Petro Borysko, Andrea Angeli, Claudiu T. Supuran   +6 more
doaj   +2 more sources

Toward Comprehensive In Vitro Evaluation of Serum Albumin Binding of Per- and Polyfluoroalkyl Substances [PDF]

Journal of Xenobiotics
Per- and polyfluoroalkyl substances (PFAS) constitute a large and chemically diverse class of synthetic compounds characterized by one or more fully fluorinated methyl or methylene groups. Many PFAS are toxic, environmentally persistent, bioaccumulative,
Hannah M. Starnes, Scott M. Belcher
doaj   +2 more sources

Discovery of Novel Inhibitors against ALS-Related SOD1(A4V) Aggregation through the Screening of a Chemical Library Using Differential Scanning Fluorimetry (DSF) [PDF]

Pharmaceuticals
Background: Cu/Zn Superoxide Dismutase 1 (SOD1) is a 32 kDa cytosolic dimeric metalloenzyme that neutralizes superoxide anions into oxygen and hydrogen peroxide.
Maria Giannakou, Ifigeneia Akrani, Angeliki Tsoka, Vassilios Myrianthopoulos, Emmanuel Mikros, Constantinos Vorgias, Dimitris G. Hatzinikolaou   +6 more
doaj   +2 more sources

Protocol for performing and optimizing differential scanning fluorimetry experiments. [PDF]

STAR Protoc, 2023
Differential scanning fluorimetry (DSF) is a widely used technique for determining the apparent melting temperature (Tma) of a purified protein. Here, we present a protocol for performing and optimizing DSF experiments. We describe steps for designing and performing the experiment, analyzing data, and optimization.
Wu T, Hornsby M, Zhu L, Yu JC, Shokat KM, Gestwicki JE.   +5 more
europepmc   +5 more sources

Fluorescent Dyes for Differential Scanning Fluorimetry of RNA. [PDF]

Chembiochem
Differential scanning fluorimetry (DSF) to determine the thermal stability of RNA has only recently been adapted from proteins using the nucleic acid dye RiboGreen. Here, we investigate the suitability of seven commercially available nucleic acid dyes for use in DSF on RNA—acridine orange, ethidium bromide, RiboGreen, OliGreen, SYBR Green II, SYBR Gold,
Blackford N, Ogunkunle VS, Silvers R.
europepmc   +3 more sources

Quantitation of autoinhibitory defects in pathogenic SHP2 mutants by differential scanning fluorimetry. [PDF]

Anal Biochem, 2023
Src-homology-2-domain-containing protein tyrosine phosphatase-2 (SHP2) is a signaling enzyme whose activity is governed by an equilibrium between autoinhibited and activated states. Regulation of SHP2 activity is critical for cellular homeostasis, and mutations that alter its autoregulatory equilibrium cause cancers and developmental disorders. Several
Serbina A, Bishop AC.
europepmc   +3 more sources