Results 21 to 30 of about 6,190 (152)
SimpleDSFviewer: A tool to analyze and view differential scanning fluorimetry data for characterizing protein thermal stability and interactions [PDF]
Protein Science, 2020 Changye Sun, Edwin Yates, Yong Li
exaly +2 more sources
Theory and applications of differential scanning fluorimetry in early-stage drug discovery. [PDF]
Biophys Rev, 2020 AbstractDifferential scanning fluorimetry (DSF) is an accessible, rapid, and economical biophysical technique that has seen many applications over the years, ranging from protein folding state detection to the identification of ligands that bind to the target protein.
Gao K, Oerlemans R, Groves MR.
europepmc +4 more sources
Determination of protein-ligand interactions using differential scanning fluorimetry. [PDF]
J Vis Exp, 2014 A wide range of methods are currently available for determining the dissociation constant between a protein and interacting small molecules. However, most of these require access to specialist equipment, and often require a degree of expertise to effectively establish reliable experiments and analyze data.
Vivoli M +3 more
europepmc +5 more sources
Formulation screening by differential scanning fluorimetry: how often does it work? [PDF]
Acta Crystallographica Section F, Structural Biology Communications, 2015 Nicholas Rosa +2 more
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Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology, 2022 Royal jelly (RJ) is produced by the hypopharingeal and mandibular glands of bees. Up to 90% of the RJ proteins are the major royal jelly proteins (MRJPs) that exhibit biological properties.
Carmen Ioana MUREŞAN +4 more
doaj +1 more source
DSFworld: A flexible and precise tool to analyze differential scanning fluorimetry data. [PDF]
Protein SciAbstractDifferential scanning fluorimetry (DSF) is a method to determine the apparent melting temperature (Tma) of a purified protein. In DSF, the raw unfolding curves from which Tma is calculated vary widely in shape and complexity. However, the tools available for calculating Tma are only compatible with the simplest of DSF curves, hindering many ...
Wu T, Gale-Day ZJ, Gestwicki JE.
europepmc +5 more sources
Molecules, 2023 Sixteen new 2-substituted quinazolines were synthesized using a straightforward methodology starting from 2-methoxybezoic acid or 3-methoxy-2-naphthoic acid.
Maria Karelou +8 more
doaj +1 more source
Vaccines, 2023 Influenza virus infections represent an ongoing public health threat as well as an economic burden. Although seasonal influenza vaccines have been available for some decades, efforts are being made to generate new efficient, flexible, and cost-effective ...
Vlad-Constantin Tofan +8 more
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Protein-adaptive differential scanning fluorimetry using conformationally responsive dyes. [PDF]
Nat BiotechnolDifferential scanning fluorimetry (DSF) is a technique that reports protein thermal stability via the selective recognition of unfolded states by fluorogenic dyes. However, DSF applications remain limited by protein incompatibilities with existing DSF dyes.
Wu T +13 more
europepmc +3 more sources
Бюллетень сибирской медицины, 2020 The aim of this work was to study the conformational changes of lactate dehydrogenase under the influence of different concentrations of intermediates (pyruvate, oxaloacetate) in the temperature gradient with the subsequent building of a mathematical ...
F. N. Gilmiyarova +7 more
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