Combining nano-differential scanning fluorimetry and microscale thermophoresis to investigate VDAC1 interaction with small molecules [PDF]
Journal of Enzyme Inhibition and Medicinal Chemistry, 2023 The mitochondrial voltage-dependent anion channel 1 (VDAC1) plays a central role in metabolism and apoptosis, which makes it a promising therapeutic target. Nevertheless, molecular mechanisms governing VDAC1 functioning remain unclear.
Hubert Gorny +5 more
doaj +7 more sources
Nano Differential Scanning Fluorimetry as a Rapid Stability Assessment Tool in the Nanoformulation of Proteins [PDF]
Pharmaceutics, 2023 The development and production of innovative protein-based therapeutics is a complex and challenging avenue. External conditions such as buffers, solvents, pH, salts, polymers, surfactants, and nanoparticles may affect the stability and integrity of ...
Sofia Lisina +5 more
doaj +6 more sources
Nano Differential Scanning Fluorimetry-Based Thermal Stability Screening and Optimal Buffer Selection for Immunoglobulin G [PDF]
Pharmaceuticals, 2021 Nano differential scanning fluorimetry (nanoDSF) is a high-throughput protein stability screening technique that simultaneously monitors protein unfolding and aggregation properties.
Soo Hyun Kim +3 more
doaj +5 more sources
Identification of relevant analytical methods for adeno-associated virus stability assessment during formulation development [PDF]
Microbiology SpectrumFormulation development for gene therapy viral vectors requires high-performance analytical methods that indicate or predict virus stability. Additionally, if testing involves multiple excipients, sample throughput must be sufficiently high, and the ...
Carina Rodenstein +3 more
doaj +2 more sources
326 Discovery and evaluation of a methylpyrazolopyrimidine antibacterial active against methicillin-resistant Staphylococcus aureus [PDF]
Journal of Clinical and Translational ScienceObjectives/Goals: Methicillin-resistant Staphylococcus aureus is a nefarious bacterial pathogen and is classified as a serious threat. MRSA is resistant to most B-lactam antibiotics (penicillins, cephalosporins, etc.).
Caitlyn Thomas +8 more
doaj +2 more sources
Measuring the Thermal Unfolding of Lysozyme: A Critical Comparison of Differential Scanning Fluorimetry and Differential Scanning Calorimetry [PDF]
ChemistryOpenThe thermal unfolding of lysozyme in aqueous solution has been analyzed by (nano) differential scanning fluorimetry (nanoDSF) and differential scanning calorimetry (DSC).
Weronika Malicka +9 more
doaj +2 more sources
Enhancing therapeutic antibody profiling: orthogonal strategies for stability and quality assessment [PDF]
Frontiers in PharmacologyToday, a variety of multivalent antibody formats can be engineered, offering significant flexibility for therapeutic and diagnostic approaches. While unmodified antibodies have evolved to be structurally stable, extensive engineering, such as domain ...
Nicole G. Metzendorf +2 more
doaj +2 more sources
Alicyclobacillin 24: a class III bacteriocin from Alicyclobacillus acidoterrestris targeting species associated with spoilage of acidic fruit-based products [PDF]
Frontiers in MicrobiologyThe thermoacidophilic Alicyclobacillus (ACB) genus includes several species responsible for spoilage of acidic fruit-based products, yet bacteriocins produced by these bacteria remain unexplored.
Inês Carvalho Leonardo +21 more
doaj +2 more sources
Chemical Thermodynamics and Thermal Analysis, 2022 Proteins fold into their most stable yet dynamic structures that enable their functions and exist in a balance between stability and instability. Protein folding and binding can be evaluated based on their thermodynamics determined using various methods.
Momoka Iiyama +4 more
doaj +1 more source
Excess of guide RNA reduces knockin efficiency and drastically increases on-target large deletions
iScience, 2023 Summary: CRISPR-Cas9 cleavage efficacy and accuracy are the main challenges gene editing faces, and they are particularly affected by the optimal formation of the ribonucleoprotein (RNP) complex.
Vanessa Chenouard +12 more
doaj +1 more source