Results 31 to 40 of about 6,190 (152)

Role of Cys residues of C-terminal SH2 domain of phosphoinositide 3-kinase in its conformational stability and CD28-binding ability

Chemical Thermodynamics and Thermal Analysis, 2022
Proteins fold into their most stable yet dynamic structures that enable their functions and exist in a balance between stability and instability. Protein folding and binding can be evaluated based on their thermodynamics determined using various methods.
Momoka Iiyama, Yoshiji Hantani, Rene H. Wink, Masataka Kuroda, Masayuki Oda   +4 more
doaj   +1 more source

Dark nanodiscs for evaluating membrane protein thermostability by differential scanning fluorimetry. [PDF]

Biophys J
Measuring protein thermostability provides valuable information on the biophysical rules that govern the structure-energy relationships of proteins. However, such measurements remain a challenge for membrane proteins. Here, we introduce a new experimental system to evaluate membrane protein thermostability.
Selvasingh JA, McDonald EF, Neufer PD, McKinney JR, Meiler J, Ledwitch KV.   +5 more
europepmc   +3 more sources

Dietary flavonoid fisetin binds human SUMO1 and blocks sumoylation of p53.

PLoS ONE, 2020
Flavonoids are plant-derived compounds that occur abundantly in fruits and vegetables and have been shown to possess potent anti-cancer, antioxidant, and anti-inflammatory properties.
Vaithish Velazhahan, Przemyslaw Glaza, Alvaro I Herrera, Om Prakash, Michal Zolkiewski, Brian V Geisbrecht, Kathrin Schrick   +6 more
doaj   +1 more source

Purification and properties of glyceraldehyde-3-phosphate dehydrogenase from the skeletal muscle of the hibernating ground squirrel, Ictidomys tridecemlineatus [PDF]

PeerJ, 2014
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the skeletal muscle of euthermic and torpid Ictidomys tridecemlineatus was purified to electrophoretic homogeneity using a novel method involving Blue-agarose and Phenyl-agarose chromatography ...
Ryan A.V. Bell, Jeffrey C. Smith, Kenneth B. Storey   +2 more
doaj   +2 more sources

Isothermal chemical denaturation assay for monitoring protein stability and inhibitor interactions

Scientific Reports, 2023
Thermal shift assay (TSA) with altered temperature has been the most widely used method for monitoring protein stability for drug research. However, there is a pressing need for isothermal techniques as alternatives.
Randa Mahran, Niklas Vello, Anita Komulainen, Morteza Malakoutikhah, Harri Härmä, Kari Kopra   +5 more
doaj   +1 more source

Investigating Buffer Effects on Lysozyme Adsorption to Borosilicate Glass

British Journal of Pharmacy, 2022
Protein adsorption refers to the accumulation and adherence of a protein to the surface of a solid, but without surface, penetration occurring. Proteins can adsorb to a variety of interfaces that are used in the manufacture, formulation, and storage of
John Donal Downey, Abina Crean, Katie B Ryan   +2 more
doaj   +1 more source

Environmental Identification of Novel Enzymes for Polyurethane and Polyamide Degradation

Angewandte Chemie, EarlyView.
Heteroatom‐containing plastics such as polyurethanes and nylons are produced at scale yet remain largely unrecycled. Biocatalysts offer a promising route to depolymerization under mild conditions. Here, we present a screening platform for the discovery of novel polyurethane‐ and nylon‐degrading enzymes from environmental samples and demonstrate ...
Malthe Kjær Bendtsen, Andreas Møllebjerg, Samuel Peña‐Díaz, Rosie Graham, Nicolai Claus Petersen, Bjørk Nolsøe Isaksen, Mathias Carstensen, Martin B. Johansen, Andreas Sommerfeldt, Allan R. Petersen, Iddi Khamisi Chuma, Cecilie Ryberg, Thomas Rea Wittenborn, Virginia Gichuru, Huabing Wang, Carsten Scavenius, Alexander Sandahl, Daniel E. Otzen   +17 more
wiley   +2 more sources

A low-cost 3D-printable differential scanning fluorometer for protein and RNA melting experiments

HardwareX, 2022
Differential scanning fluorimetry (DSF) is a widely used biophysical technique with applications to drug discovery and protein biochemistry. DSF experiments are commonly performed in commercial real-time polymerase chain reaction (qPCR) thermal cyclers ...
Fabian Barthels, Stefan J. Hammerschmidt, Tim R. Fischer, Collin Zimmer, Elisabeth Kallert, Mark Helm, Christian Kersten, Tanja Schirmeister   +7 more
doaj   +1 more source

Characterization of aminoacyl-tRNA synthetase stability and substrate interaction by differential scanning fluorimetry. [PDF]

Methods, 2017
Differential scanning fluorimetry (DSF) is a fluorescence-based assay to evaluate protein stability by determining protein melting temperatures. Here, we describe the application of DSF to investigate aminoacyl-tRNA synthetase (AARS) stability and interaction with ligands.
Abbott JA, Livingston NM, Egri SB, Guth E, Francklyn CS.   +4 more
europepmc   +4 more sources

Identification of Nuclear Receptor Ligands using Differential Scanning Fluorimetry [PDF]

Biophysical Journal, 2012
Identification of ligands that interact with nuclear receptors (NRs) is both a major biological problem and an important initial step in drug discovery. Several in vitro and in vivo techniques are commonly used to screen ligand candidates against nuclear receptors, however none of the current assays allow screening without modification of either the ...
Reinking, Jeff, Dipaola, Nicholas, DeSantis, Kara, Magerkurth, Marissa, Benkwitt, Sarah, Reed, Aaron   +5 more
openaire   +1 more source