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A Thermostable Cyclodextrin Glycosyltransferase from Thermoanaerobacter sp. 5K

Current Biotechnology, 2015
Cyclodextrin glycosyltransferase (CGTase) from the thermophilic anaerobe Thermoanaerobacter sp. 5K was purified and characterized. The enzyme was purified with ammonium sulfate precipitation followed by α-CD-bound, epoxy-activated Sepharose 6B affinity chromatography. Molecular weight of the purified enzyme was 70.6 kDa. The enzyme had optimal activity
Ayse Avci   +5 more
openaire   +1 more source

Functional role of β domain in the Thermoanaerobacter tengcongensis glucoamylase

Applied Microbiology and Biotechnology, 2013
Thermoanaerobacter tengcongensis MB4 glucoamylase (TteGA) contains a catalytic domain (CD), which is structurally similar to eukaryotic GA, and a β domain (BD) with ambiguous function. Firstly, BD is found to be essential to TteGA activity because CD alone could not hydrolyze soluble starch.
Zilong, Li   +5 more
openaire   +2 more sources

Cloning and sequencing of kojibiose phosphorylase gene from Thermoanaerobacter brockii ATCC35047

Journal of Bioscience and Bioengineering, 2004
A gene encoding kojibiose phosphorylase was cloned from Thermoanaerobacter brockii ATCC35047. The kojP gene encodes a polypeptide of 775 amino acid residues. The deduced amino acid sequence was homologous to those of trehalose phosphorylase from T. brockii and maltose phosphorylases from Bacillus sp.
Takuo, Yamamoto   +8 more
openaire   +2 more sources

Proteomic analysis on the temperature‐dependent complexes in Thermoanaerobacter tengcongensis

PROTEOMICS, 2009
AbstractIt is generally accepted that protein complexes play an active role in avoiding the protein degradation of the thermophiles. Thermoanaerobacter tengcongensis was cultured at three different temperatures (55, 75 and 80°C) and the extracts of protein complexes were prepared.
Bo, Meng   +11 more
openaire   +2 more sources

[14] Chaperonins from Thermoanaerobacter species

1998
Robert K. Scopes, Kaye Truscott
openaire   +1 more source

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