Results 61 to 70 of about 6,565 (220)

A Robust Bioprocess for the Global Incorporation of Noncanonical Amino Acids in Auxotrophic Hosts Produces Labeled Proteins at the Gram Scale

ChemBioChem, Volume 27, Issue 1, January 2026.
A robust bioprocess enables global incorporation of noncanonical amino acids in both shake flask and bioreactor cultures, producing gram‐per‐liter titers of efficiently labeled proteins. A versatile Escherichia coli BL21(DE3) auxotroph platform supports broad compatibility with various noncanonical amino acids and target proteins, facilitating flexible
Patrik Fladischer, Niklaus Anderhuber, Octavian Murgu, Altijana Hromić‐Jahjefendić, Tea Pavkov‐Keller, Karl Gruber, Juergen Mairhofer, Gerald Striedner, Birgit Wiltschi   +8 more
wiley   +1 more source

Harnessing Molecular Recognition for Small‐Molecule‐Mediated Reversible Photochemical Control Over mRNA Translation

Angewandte Chemie, Volume 137, Issue 22, May 26, 2025.
A small molecule ligand undergoes traceless, reversible photocrosslinking to PreQ1 RNA aptamer in a selective manner, dependent on both the RNA sequence/structure and ligand chemical structure. A caged mRNA construct incorporating the PreQ1 aptamer and an eGFP or p53 coding sequence remained translationally repressed until exposure to 302 nm light ...
Shaifaly Parmar, Logan Tenney, Xiao Liang, John T. Routzahn IV, Christopher D. Sibley, John S. Schneekloth Jr.   +5 more
wiley   +2 more sources

Enrichment and microbial characterization of syngas converting anaerobic cultures [PDF]

, 2013
Bioconversion of recalcitrant biomass/waste into bulk chemicals or biofuels is often not feasible. By gasification of these materials, syngas (mainly composed of CO2, CO and H2) is generated and can be used for the production of high value compounds by ...
Alves, J. I., Alves, M. M., Plugge, Caroline M., Sousa, D. Z., Stams, Alfons Johannes Maria, Visser, Michael   +5 more
core  

Ether- and Ester-Bound iso-Diabolic Acid and Other Lipids in Members of Acidobacteria Subdivision 4 [PDF]

, 2014
Recently, iso-diabolic acid (13,16-dimethyl octacosanedioic acid) has been identified as a major membrane-spanning lipid of subdivisions 1 and 3 of the Acidobacteria, a highly diverse phylum within the Bacteria.
Bryant, D.A., Dunfield, P.F., Foesel, B.U., Hopmans, E.C., Houghton, K., Overmann, J., Rijpstra, W.I.C., Sinninghe Damsté, J.S., Stott, M., Tank, M., Wüst, P.K.   +10 more
core   +2 more sources

Biological Conversion of Formate to Organic Compounds: Toward a Sustainable Formate Bioeconomy

Carbon Energy, Volume 8, Issue 1, January 2026.
Formate bioconversion plays a crucial role in achieving renewable resource utilization and sustainable development. To tap its full potential, it is important to identify the most appropriate microbial hosts for incorporating formate into building blocks, design the most promising metabolic pathways for transmitting formate into central carbon ...
Jinyi Qian, Tiantian Chai, Chunlei Zhao, Xiulai Chen   +3 more
wiley   +1 more source

Systematic characterization of a novel gal operon in Thermoanaerobacter tengcongensis [PDF]

Microbiology, 2009
On the basis of theThermoanaerobacter tengcongensisgenome, a novel type ofgaloperon was deduced. The gene expression and biochemical properties of this operon were further characterized. RT-PCR analysis of the intergenic regions suggested that the transcription of thegaloperon was continuous.
Zhong, Qian, Bo, Meng, Quanhui, Wang, Zhuowei, Wang, Chuanqi, Zhou, Qian, Wang, Shuyang, Tu, Liang, Lin, Yanhe, Ma, Siqi, Liu   +9 more
openaire   +2 more sources

Residue-Specific Incorporation of the Non-Canonical Amino Acid Norleucine Improves Lipase Activity on Synthetic Polyesters

Frontiers in Bioengineering and Biotechnology, 2022
Environmentally friendly functionalization and recycling processes for synthetic polymers have recently gained momentum, and enzymes play a central role in these procedures.
Karolina Haernvall, Patrik Fladischer, Patrik Fladischer, Heidemarie Schoeffmann, Sabine Zitzenbacher, Tea Pavkov-Keller, Tea Pavkov-Keller, Tea Pavkov-Keller, Tea Pavkov-Keller, Karl Gruber, Karl Gruber, Karl Gruber, Michael Schick, Motonori Yamamoto, Andreas Kuenkel, Doris Ribitsch, Doris Ribitsch, Georg M. Guebitz, Georg M. Guebitz, Birgit Wiltschi, Birgit Wiltschi, Birgit Wiltschi   +21 more
doaj   +1 more source

Physiological roles of pyruvate ferredoxin oxidoreductase and pyruvate formate-lyase in Thermoanaerobacterium saccharolyticum JW/SL-YS485 [PDF]

, 2015
Background: Thermoanaerobacter saccharolyticum is a thermophilic microorganism that has been engineered to produce ethanol at high titer (30–70 g/L) and greater than 90 % theoretical yield.
Anthony A Lanahan, Daniel G Olson, Jilai Zhou, Jonathan Lo, Lee R Lynd, Liang Tian, Sean Jean-Loup Murphy   +6 more
core   +2 more sources

Recent Progress in CO2 Conversion: An Overview of Catalytic Strategies for Sustainable Fuel and Chemical Synthesis

SmartMat, Volume 6, Issue 6, December 2025.
This review systematically outlines recent advances in the catalytic strategies for converting CO₂ into valuable products, including photocatalysis, electrocatalysis, CO2 hydrogenation, photothermal catalysis, non‐thermal plasma, and biocatalytic processes.
An Zhang, Yanming Cai, Liang Guo, Chao Wang, Qingbo Wa, Yanping Xu, Jian Zhou, Hongshuang Fan, Ziyun Li, Xinyue Long, Jiabei Tang, Zijian Li, Li Zhai, Zhenyu Shi, Wei Zhai, Yuhui Tian, Shuai Bi, Hao Wei, Mingliang Hu, Yang Xu, Yiran Zhang, Yu Zhang, Ruiying Ding, Junjun Li, Shuangqun Chen, Jianfeng Huang, Zhanxi Fan, Xingchen Jiao, Guigao Liu, Haiqing Wang, Haifeng Tian, Jingyu Pang, Kuncheng Zhang, Weisong Liu, Huijuan Cui, Lingling Zhang, Xiaofu Sun, Jianling Zhang, Dan Ren, Sheng Zhang, Lu Qiao, Zhe Weng, Hongmei Li, Min Liu, Miao Zhong, Lei Wang, Jun Gu, Hao Bin Wu, Bolong Huang, Weijia Zhou, Chuan Xia, Min‐Rui Gao, Changbin Zhang, Rong Cao, Wei Chen, Yuan‐Biao Huang, Yongye Liang, Ya‐Qian Lan, Guoxiong Wang, Baoyu Xia, Yujie Xiong, Xinbin Ma, Tongbu Lu, Jinhua Ye, Yi Xie, Buxing Han, Jiawei Liu, Zhicheng Zhang, Hua Zhang   +68 more
wiley   +1 more source

Construction of a Bacterial Cellulase Cocktail for Saccharification of Regenerated Cellulose and Pretreated Corn Stover

BioResources, 2015
To apply bacterial cellulases for efficient saccharification of biomass, three Clostridium thermocellum cellulases and a Thermoanaerobacter brockii β-1,4-glucosidase were synthesized in Escherichia coli, and the proportions among them were optimized ...
Alei Geng, Jian Wu, Rongrong Xie, Xia Li, Fuxiang Chang, Jianzhong Sun   +5 more
doaj   +1 more source