Molecular Basis of the Electron Bifurcation Mechanism in the [FeFe]-Hydrogenase Complex HydABC. [PDF]
J Am Chem Soc, 2023 Electron bifurcation is a fundamental energy coupling mechanism widespread in microorganisms that thrive under anoxic conditions. These organisms employ hydrogen to reduce CO2, but the molecular mechanisms have remained enigmatic.
Katsyv A +10 more
europepmc +5 more sources
A purified energy-converting hydrogenase from Thermoanaerobacter kivui demonstrates coupled H+-translocation and reduction in vitro. [PDF]
J Biol Chem, 2022 Energy-converting hydrogenases (Ech) are ancient, membrane-bound enzymes that use reduced ferredoxin (Fd) as an electron donor to reduce protons to molecular H2. Experiments with whole cells, membranes and vesicle-fractions suggest that proton reduction is coupled to proton translocation across the cytoplasmatic membrane, but this has never been ...
Katsyv A, Müller V.
europepmc +5 more sources
CO Metabolism in the Thermophilic Acetogen Thermoanaerobacter kivui. [PDF]
Appl Environ Microbiol, 2016 ABSTRACT The thermophilic acetogenic bacterium Thermoanaerobacter kivui , previously described not to use carbon monoxide as a carbon and energy source, was adapted to grow on CO. This was achieved by using a preculture grown on H 2 plus CO 2 and by ...
Weghoff MC, Müller V.
europepmc +5 more sources
A Genetic System for the Thermophilic Acetogenic Bacterium Thermoanaerobacter kivui. [PDF]
Appl Environ Microbiol, 2018 ABSTRACT Thermoanaerobacter kivui is one of the very few thermophilic acetogenic microorganisms. It grows optimally at 66°C on sugars but also lithotrophically with H 2 + CO 2 or with CO, producing acetate as the major product.
Basen M, Geiger I, Henke L, Müller V.
europepmc +5 more sources
Utilization of formic acid by extremely thermoacidophilic archaea species. [PDF]
Microb BiotechnolThe exploration of novel hosts with the ability to assimilate formic acid, a C1 substrate that can be produced from renewable electrons and CO2, is of great relevance for developing novel and sustainable biomanufacturing platforms.
Tejedor-Sanz S, Song YE, Sundstrom ER.
europepmc +4 more sources
Obligate autotrophy at the thermodynamic limit of life in a new acetogenic bacterium [PDF]
Frontiers in Microbiology, 2023 One of the important current issues of bioenergetics is the establishment of the thermodynamic limits of life. There is still no final understanding of what is the minimum value of the energy yield of a reaction that is sufficient to be used by an ...
Evgenii N. Frolov +6 more
doaj +2 more sources
Adaptive laboratory evolution of a thermophile toward a reduced growth temperature optimum [PDF]
Frontiers in Microbiology, 2023 Thermophily is an ancient trait among microorganisms. The molecular principles to sustain high temperatures, however, are often described as adaptations, somewhat implying that they evolved from a non-thermophilic background and that thermophiles, i.e ...
Maria Lehmann +5 more
doaj +2 more sources
A temperature dependent pilin promoter for production of thermostable enzymes in Thermus thermophilus [PDF]
Microbial Cell Factories, 2023 Background Enzymes from thermophiles are of great interest for research and bioengineering due to their stability and efficiency. Thermophilic expression hosts such as Thermus thermophilus [T.
Lennart Kirchner +2 more
doaj +2 more sources
Leveraging substrate flexibility and product selectivity of acetogens in two-stage systems for chemical production [PDF]
Microbial Biotechnology, Volume 16, Issue 2, Page 218-237, February 2023., 2022 Carbon dioxide (CO2 ) stands out as sustainable feedstock for developing a circular carbon economy whose energy supply could be obtained by boosting the production of clean hydrogen from renewable electricity.
Bernacchi, Sebastien +5 more
core +2 more sources
Biotechnology for Biofuels, 2020 Background In times of global climate change, the conversion and capturing of inorganic CO2 have gained increased attention because of its great potential as sustainable feedstock in the production of biofuels and biochemicals.
Fabian M. Schwarz, Volker Müller
doaj +2 more sources