A thermostable Cas9-based genome editing system for thermophilic acetogenic bacterium Thermoanaerobacter kivui [PDF]
Thermoanaerobacter kivui is a thermophilic acetogenic bacterium capable of thriving at elevated temperatures up to 66°C. It metabolizes carbohydrates such as glucose, mannose, and fructose and can also grow lithotrophically utilizing hydrogen (H2) and ...
Yilin Le +7 more
doaj +7 more sources
Electron carriers involved in autotrophic and heterotrophic acetogenesis in the thermophilic bacterium Thermoanaerobacter kivui [PDF]
AbstractThermoanaerobacter kivuiis an acetogenic model organism that reduces CO2with electrons derived from H2or CO, or from organic substrates in the Wood–Ljugdahl pathway (WLP). For the calculation of ATP yields, it is necessary to know the electron carriers involved in coupling of the oxidative and reductive parts of metabolism.
Alexander Katsyv +2 more
exaly +7 more sources
The monofunctional CO dehydrogenase CooS is essential for growth of Thermoanaerobacter kivui on carbon monoxide [PDF]
AbstractThermoanaerobacter kivui is a thermophilic acetogen that can grow on carbon monoxide as sole carbon and energy source. To identify the gene(s) involved in CO oxidation, the genome sequence was analyzed. Two genes potentially encoding CO dehydrogenases were identified.
Alexander Katsyv +2 more
exaly +7 more sources
A Genetic System for the Thermophilic Acetogenic Bacterium Thermoanaerobacter kivui [PDF]
ABSTRACT Thermoanaerobacter kivui is one of the very few thermophilic acetogenic microorganisms. It grows optimally at 66°C on sugars but also lithotrophically with H 2 + CO 2 or with CO, producing acetate as the major product.
Mirko Basen, Volker Müller
exaly +6 more sources
CO Metabolism in the Thermophilic Acetogen Thermoanaerobacter kivui [PDF]
ABSTRACT The thermophilic acetogenic bacterium Thermoanaerobacter kivui , previously described not to use carbon monoxide as a carbon and energy source, was adapted to grow on CO. This was achieved by using a preculture grown on H 2 plus CO 2 and by ...
Marie Charlotte Schoelmerich +1 more
exaly +6 more sources
Characterization of ferredoxins from the thermophilic, acetogenic bacterium Thermoanaerobacter kivui [PDF]
Ferredoxins are key proteins in anaerobic metabolism. In early life forms such as the acetogenic bacteria, low potential ferredoxins serve to reduce carbon dioxide. Acetogens contain multiple genes each potentially encoding a different ferredoxin, but this and their function in metabolism remained elusive.
Alexander Katsyv +5 more
wiley +5 more sources
A purified energy-converting hydrogenase from Thermoanaerobacter kivui demonstrates coupled H+-translocation and reduction in vitro. [PDF]
Energy-converting hydrogenases (Ech) are ancient, membrane-bound enzymes that use reduced ferredoxin (Fd) as an electron donor to reduce protons to molecular H2. Experiments with whole cells, membranes and vesicle-fractions suggest that proton reduction is coupled to proton translocation across the cytoplasmatic membrane, but this has never been ...
Katsyv A, Müller V.
europepmc +6 more sources
Formate-driven H2 production by whole cells of Thermoanaerobacter kivui. [PDF]
Abstract Background In times of global warming there is an urgent need to replace fossil fuel-based energy vectors by less carbon dioxide (CO2)-emitting alternatives. One attractive option is the use of molecular hydrogen (H2) since its combustion emits water (H2O) and not CO2.
Burger Y, Schwarz FM, Müller V.
europepmc +5 more sources
A genome-guided analysis of energy conservation in the thermophilic, cytochrome-free acetogenic bacterium Thermoanaerobacter kivui. [PDF]
BMC Genomics, 15 (1)
Hess V +4 more
europepmc +9 more sources
Sequencing of a <i>Thermoanaerobacter kivui</i> isolate from DSMZ stock: major differences with reference assembly. [PDF]
ABSTRACT Thermoanaerobacter kivui DSM 2030 is an industrially relevant microbe, whose genome has been available since 2014. As the stock obtained from DSMZ appeared to be multiclonal, we obtained a complete genome sequence by de novo assembly of an isolate from
Hocq R, Thallinger GG, Pflügl S.
europepmc +4 more sources

