Results 21 to 30 of about 322 (85)

A fluorescent reporter system for anaerobic thermophiles [PDF]

Frontiers in Bioengineering and Biotechnology, 2023
Owing to their inherent capacity to make invisible biological processes visible and quantifiable, fluorescent reporter systems have numerous applications in biotechnology. For classical fluorescent protein systems (i.e., GFP and derivatives), chromophore
Rémi Hocq, Rémi Hocq, Sara Bottone, Sara Bottone, Arnaud Gautier, Arnaud Gautier, Stefan Pflügl, Stefan Pflügl   +7 more
doaj   +2 more sources

Obligate autotrophy at the thermodynamic limit of life in a new acetogenic bacterium [PDF]

Frontiers in Microbiology, 2023
One of the important current issues of bioenergetics is the establishment of the thermodynamic limits of life. There is still no final understanding of what is the minimum value of the energy yield of a reaction that is sufficient to be used by an ...
Evgenii N. Frolov, Alexander G. Elcheninov, Alexandra V. Gololobova, Stepan V. Toshchakov, Andrei A. Novikov, Alexander V. Lebedinsky, Ilya V. Kublanov   +6 more
doaj   +2 more sources

DNA uptake from a laboratory environment drives unexpected adaptation of a thermophile to a minor medium component [PDF]

ISME Communications, 2023
DNA uptake is widespread among microorganisms and considered a strategy for rapid adaptation to new conditions. While both DNA uptake and adaptation are referred to in the context of natural environments, they are often studied in laboratories under ...
Benjamin Zeldes, Anja Poehlein, Surbhi Jain, Christoph Baum, Rolf Daniel, Volker Müller, Mirko Basen   +6 more
doaj   +2 more sources

Adaptive laboratory evolution of a thermophile toward a reduced growth temperature optimum [PDF]

Frontiers in Microbiology, 2023
Thermophily is an ancient trait among microorganisms. The molecular principles to sustain high temperatures, however, are often described as adaptations, somewhat implying that they evolved from a non-thermophilic background and that thermophiles, i.e ...
Maria Lehmann, Christoph Prohaska, Benjamin Zeldes, Anja Poehlein, Rolf Daniel, Mirko Basen   +5 more
doaj   +2 more sources

A temperature dependent pilin promoter for production of thermostable enzymes in Thermus thermophilus [PDF]

Microbial Cell Factories, 2023
Background Enzymes from thermophiles are of great interest for research and bioengineering due to their stability and efficiency. Thermophilic expression hosts such as Thermus thermophilus [T.
Lennart Kirchner, Volker Müller, Beate Averhoff   +2 more
doaj   +2 more sources

Genomic and Transcriptomic Insights Into the Autotrophic Metabolism on H₂ + CO₂ or CO of the Thermophilic Acetogenic Model Organism Moorella thermoacetica. [PDF]

Environ Microbiol
Moorella thermoacetica is the model organism for elucidation of acetogenesis. The reduction of CO2 to acetate is well studied, but how redox carrier balancing and energy conservation are maintained during autotrophic growth and how H2 is oxidised remains enigmatic.
Rosenbaum FP, Poehlein A, Daniel R, Müller V.   +3 more
europepmc   +2 more sources

ICECREAM: high-fidelity equivariant cryo-electron tomography. [PDF]

Acta Crystallogr D Struct Biol
We present ICECREAM, a self‐supervised approach that achieves substantially better denoising and more reliable missing‐wedge recovery in cryo‐ET, while reducing training and inference time relative to comparable baselines.Cryo‐electron tomography (cryo‐ET) visualizes 3D cellular architecture in its near‐native state.
Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I.   +4 more
europepmc   +2 more sources

Comparative Physiology and Genomics of Thermincola and Carboxydocella Strains and Description of Two Novel Isolates. [PDF]

Environ Microbiol
Two novel thermophilic bacteria, Thermincola strain AZ34E and Carboxydocella strain AZ29I, were isolated from hydrothermal environments and shown to oxidise carbon monoxide via the water‐gas shift reaction. Comparative genomic and phylogenetic analyses revealed that all currently described isolates within Thermincola and Carboxydocella each represent a
Galani A, Antony Venancius M, Sipkema D, Sousa DZ.   +3 more
europepmc   +2 more sources

Methanol and Carbon Monoxide Metabolism of the Thermophile Moorella caeni. [PDF]

Environ Microbiol
An omics approach was used to elucidate the C1 metabolism of Moorella caeni DSM 21394 T, which exhibits acetogenic metabolism on methanol and hydrogenogenic metabolism on CO. Our results suggest that respiratory Complex I serves as the primary respiratory enzyme in methanol‐grown cells.
Vecchini Santaella NA, Stams AJM, Sousa DZ.   +2 more
europepmc   +2 more sources

Effects of Nitrate on Hydrogenogenic Carbon Monoxide Oxidation in Parageobacillus thermoglucosidasius. [PDF]

Environ Microbiol Rep
Parageobacillus thermoglucosidasius hierarchically utilises nitrate respiration and hydrogenogenic carbon monoxide (CO) oxidation. Nitrate suppresses CO oxidation and promotes rapid cell growth through hydrogen‐dependent nitrate reduction. For this bacterium, CO serves as a supplemental energy source in environments lacking alternative electron ...
Katayama YA, Imaura Y, Inoue M, Okamoto S, Sako Y, Kamikawa R, Yoshida T.   +6 more
europepmc   +2 more sources