Protocol for freezing thermotolerant cells and maintaining thermotolerance following thawing
Two independent laboratories have demonstrated that suspension-grown, Chinese hamster ovary (CHO) cells can be made thermotolerant, frozen and subsequently thawed such that they still express thermotolerance. Thermotolerance was determined as the ability to protect cells against hyperthermic cell killing (colony formation assay) and the ability to ...
M J, Borrelli +3 more
openaire +2 more sources
Coral reefs are in global decline mainly due to increasing sea surface temperatures triggering coral bleaching. Recently, high salinity has been linked to increased thermotolerance and decreased bleaching in the sea anemone coral model Aiptasia. However,
Hagen M. Gegner +8 more
doaj +1 more source
QTL analysis of high thermotolerance with superior and downgraded parental yeast strains reveals new minor QTLs and converges on novel causative alleles involved in RNA processing [PDF]
Revealing QTLs with a minor effect in complex traits remains difficult. Initial strategies had limited success because of interference by major QTLs and epistasis. New strategies focused on eliminating major QTLs in subsequent mapping experiments.
Clement, Lieven +7 more
core +3 more sources
Nitric oxide (NO) and hydrogen sulfide (H2S) are novel signaling molecules, which participate in plant growth, development, and response to stress.
Yu-Ying Sun +11 more
doaj +1 more source
Varied effects of algal symbionts on transcription factor NF-κB in a sea anemone and a coral: possible roles in symbiosis and thermotolerance [PDF]
Many cnidarians, including the reef-building corals, undergo symbiotic mutualisms with photosynthetic dinoflagellate algae of the family Symbiodiniaceae.
Benson, Brooke E. +11 more
core +1 more source
His+ reversions Caused in Salmonella typhimurium by different types of ionizing radiation [PDF]
The yield of his+ reversions in the Ames Salmonella tester strain TA2638 has been determined for 60Co γ rays, 140 kV X rays, 5.4 keV characteristic X rays, 2.2 MeV protons, 3.1 MeV α particles, and 18 MeV/U Fe ions.
Fitzek, M. +3 more
core +1 more source
How rice adapts to high temperatures
High-temperature stress affects crop yields worldwide. Identifying thermotolerant crop varieties and understanding the basis for this thermotolerance would have important implications for agriculture, especially in the face of climate change. Rice (Oryza
Huimin Ren +5 more
doaj +1 more source
Hyperthermia combined with chemotherapy - Biological rationale, clinical application, and treatment results [PDF]
There is substantial evidence from preclinical data that the antitumor cytotoxicity of selected chemotherapeutic agents either alone or combined with radiation can be enhanced by appropriate heat exposure (40-44 degrees C) of cells or tumor tissues ...
Issels, Rolf-Dieter
core +1 more source
Summary: Global warming and emerging plant diseases challenge agricultural food/feed production. We identify mechanism(s) regulating both plant thermotolerance and disease resistance.
Bikram Datt Pant +4 more
doaj +1 more source
A rapid assay for assessing bacterial effects on Arabidopsis thermotolerance
Background The role of beneficial microbes in mitigating plant abiotic stress has received considerable attention. However, the lack of a reproducible and relatively high-throughput screen for microbial contributions to plant thermotolerance has greatly ...
Jun Hyung Lee +6 more
doaj +1 more source

