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2021
Bacteria of the genus Thermus were first isolated from hot springs in Yellowstone National Park, US at temperatures of 53 to 86°C and pH values between 8 and 9, and allocated to the type species, Thermus aquaticus. A low concentration of organic matter in the medium, together with an incubation temperature of 70 to 75°C, was emphasized as important for
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Bacteria of the genus Thermus were first isolated from hot springs in Yellowstone National Park, US at temperatures of 53 to 86°C and pH values between 8 and 9, and allocated to the type species, Thermus aquaticus. A low concentration of organic matter in the medium, together with an incubation temperature of 70 to 75°C, was emphasized as important for
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Isolation and characterization of Thermus bacteriophages
Archives of Virology, 2005One-hundred-fifteen bacteriophage strains were isolated from alkaline hot springs in Iceland, New Zealand, Russia (Kamchatka), and the U.S.A. The phages belonged to the Myoviridae, Siphoviridae, Tectiviridae, and Inoviridae families. Over 50% of isolates were isometric or filamentous.
Hans-Wolfgang Ackermann+2 more
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Extremophiles, 2000
The Thermus thermophilus TH125 alpha-galactosidase gene, agaT, and flanking sequences were cloned in Escherichia coli and sequenced as well as flanking sequences of the previously cloned agaT from Thermus brockianus ITI360. Different structures of putative alpha-galactosidase operons in the two Thermus strains were revealed.
Hildegard Watzlawick+2 more
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The Thermus thermophilus TH125 alpha-galactosidase gene, agaT, and flanking sequences were cloned in Escherichia coli and sequenced as well as flanking sequences of the previously cloned agaT from Thermus brockianus ITI360. Different structures of putative alpha-galactosidase operons in the two Thermus strains were revealed.
Hildegard Watzlawick+2 more
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The Taxonomy and Identification of Thermus
1995Bacteria of the genus Thermus were first isolated in the late 1960s from hot springs in Japan and the USA. Thermus strains also appear to be widespread in neutral, hot, aqueous artificial environments such as domestic and industrial hot water systems and thermally polluted streams.
Ralph A. D. Williams, Richard J. Sharp
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Environmental Science & Technology, 2001
Thermus aquaticus and Thermus thermophilus, common inhabitants of terrestrial hot springs and thermally polluted domestic and industrial waters, have been found to rapidly oxidize arsenite to arsenate. Field investigations at a hot spring in Yellowstone National Park revealed conserved total arsenic transport and rapid arsenite oxidation occurring ...
Jillian F. Banfield+4 more
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Thermus aquaticus and Thermus thermophilus, common inhabitants of terrestrial hot springs and thermally polluted domestic and industrial waters, have been found to rapidly oxidize arsenite to arsenate. Field investigations at a hot spring in Yellowstone National Park revealed conserved total arsenic transport and rapid arsenite oxidation occurring ...
Jillian F. Banfield+4 more
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Enzymes of Thermus and Their Properties
1995Microorganisms have evolved and adapted to survive a great variety of conditions. The Thermus species are extreme thermophiles, which can be isolated from hot springs where they grow at temperatures up to 82 °C. Proteins, enzymes, organelles, and DNA purified from strains of Thermus are generally more resistant to heat than their mesophilic equivalents
Doug Cossar, Melanie L. Duffield
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Growth kinetics of Thermus thermophilus [PDF]
The nonsporulating extreme thermophile Thermus thermophilus was grown in continuous culture at dilution rates up to 2.65 h−1 at 75°C and pH 6.9 on complex medium. Concomitantly very low yield (Y=0.12 g cell dry weight g−1 utilized organic carbon) and incomplete substrate utilization (always less than 45%) were found. In batch cultures T.
S. Cometta+2 more
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Physiology and Metabolism of Thermus
1995The metabolism of thermophilic bacteria was extensively reviewed in 1979 by Ljungdahl. More recent books, monographs, and reviews that bear on the physiology of Thermus include those of Gould and Corry (1986), Brock (1986), Herbert and Codd (1986), Bergquist et al.
Richard J. Sharp+2 more
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Overproduction of carotenoids in Thermus thermophilus
Journal of Fermentation and Bioengineering, 1994Phytoene synthase encoded by the crtB gene is one of the rate-limiting enzymes for carotenoid production in Thermus thermophilus. We introduced a multicopy recombinant plasmid, pCOP1, in which the Thermus crtB gene was cloned, into carotenoid overproducing mutants of T. thermophilus.
Takayuki Hoshino+2 more
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Purification and characterization of Thermus thermophilus UvrD
Extremophiles, 2003The DNA helicase UvrD (helicase II) protein plays an important role in nucleotide excision repair, mismatch repair, rolling circular plasmid replication, and in DNA replication. A homologue of the Escherichia coli uvrD gene was previously identified in Thermus thermophilus; however, to date, a UvrD helicase has not been purified and characterized from ...
Tommie V. McCarthy, Ruairi Collins
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