Results 41 to 50 of about 20,900 (207)

Horizontal Gene Transfer in Thermus spp.

Current Issues in Molecular Biology, 2018
The small amount of genetic content in thermophiles generally limits their adaptability to environmental changes. In Thermus spp., very active horizontal gene transfer (HGT) mechanisms allow the rapid spread of strain-specific adaptive gene modules among the entire population.
Blesa, Alba, Averhoff, Beate, José, Berenguer   +3 more
openaire   +4 more sources

In Vivo Biosynthesis and Direct Incorporation of Non‐Canonical Amino Acids into Proteins

ChemBioChem, Accepted Article.
Autonomous cells are engineered biological systems capable of biosynthesising and directly incorporating non‐canonical amino acids (ncAAs) into proteins. These systems have the potential to extend the applicability of the genetic code to enable large‐scale fermentative production of proteins carrying ncAAs.
Ivana Drienovska, Jan Hendrik Illies, T. Moritz Weber   +2 more
wiley   +1 more source

CarD uses a minor groove wedge mechanism to stabilize the RNA polymerase open promoter complex

eLife, 2015
A key point to regulate gene expression is at transcription initiation, and activators play a major role. CarD, an essential activator in Mycobacterium tuberculosis, is found in many bacteria, including Thermus species, but absent in Escherichia coli. To
Brian Bae, James Chen, Elizabeth Davis, Katherine Leon, Seth A Darst, Elizabeth A Campbell   +5 more
doaj   +1 more source

Targeting the Tumor Microbiota in Cancer Therapy Basing on Nanomaterials

Exploration, EarlyView.
Intra‐tumoral microbiota, which is a potential component of the tumor microenvironment, has been emerging as a key participant and driving factor in cancer. This article reviews the latest progress in this field, including the microbial community within tumors and its pro‐cancer mechanisms, as well as the anti‐tumor strategies targeting intra‐tumoral ...
Yanan Niu, Junya Feng, Jie Ma, Tixian Xiao, Wei Yuan   +4 more
wiley   +1 more source

Specificity-Enhanced Hot-Start PCR: Addition of Double-Stranded DNA Fragments Adapted to the Annealing Temperature

BioTechniques, 2000
A new method to produce hot-start conditions in PCR is described. Short double-stranded DNA fragments were found to inhibit the activity of DNA polymerases from Thermus aquaticus and Thermus flavus.
Peter Kainz, Angela Schmiedlechner, Hans Bernd Strack   +2 more
doaj   +1 more source

Cryo-electron microscopy reveals two distinct type IV pili assembled by the same bacterium

Nature Communications, 2020
Type IV pili are flexible filaments on the surface of bacteria, consisting of a helical assembly of pilin proteins. Here, Neuhaus et al. show that the bacterium Thermus thermophilus produces two forms of type IV pilus, differing in structure, protein ...
Alexander Neuhaus, Muniyandi Selvaraj, Ralf Salzer, Julian D. Langer, Kerstin Kruse, Lennart Kirchner, Kelly Sanders, Bertram Daum, Beate Averhoff, Vicki A. M. Gold   +9 more
doaj   +1 more source

Atypical features of Thermus thermophilus succinate:quinone reductase. [PDF]

PLoS ONE, 2013
The Thermus thermophilus succinate:quinone reductase (SQR), serving as the respiratory complex II, has been homologously produced under the control of a constitutive promoter and subsequently purified.
Olga Kolaj-Robin, Mohamed R Noor, Sarah R O'Kane, Frauke Baymann, Tewfik Soulimane   +4 more
doaj   +1 more source

Supervised Learning of Protein Melting Temperature: Cross‐Species vs. Species‐Specific Prediction

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT Protein melting temperatures are important proxies for stability, and frequently probed in protein engineering campaigns, for instance for enzyme discovery and protein optimization. With the emergence of large datasets of melting temperatures for diverse natural proteins, it has become possible to train models to predict this quantity, and the
Sebastián García López, Jesper Salomon, Wouter Boomsma   +2 more
wiley   +1 more source

The Impact of Whole‐Animal Fluid Preservation on the Observed Gut Microbiome of Vertebrates: Implications for the Use of Museum Specimens in Microbiome Research

Molecular Ecology Resources, EarlyView.
ABSTRACT The vertebrate gut houses diverse microbial communities that provide insights into their host's ecological and evolutionary histories. Nevertheless, microbiome research has not been distributed equally across host taxonomy, geography and timescales. The millions of fluid‐preserved specimens stored in natural history museums worldwide represent
Samantha S. Fontaine, Stevie R. Kennedy‐Gold, Kurt J. Regester, Jennifer A. Sheridan, Kevin D. Kohl   +4 more
wiley   +1 more source

Allosteric Inhibition of Thermus Thermophilus Phosphofructokinase [PDF]

Biophysical Journal, 2010
Thermus thermophilus phosphofructokinase (TtPFK) comes from an extreme thermophile and exhibits entropically-driven inhibition by phosphoenolpyruvate (PEP). Interestingly, a PFK from the moderate thermophile Bacillus stearothermophilus also exhibits entropically-driven inhibition, while enthalphically-driven inhibition is observed for PFK from ...
Maria Shubina-McGresham, Gregory D. Reinhart   +1 more
openaire   +2 more sources