Results 61 to 70 of about 20,900 (207)

TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol

Nature Communications, 2016
Single cell genomic analysis needs DNA amplification with high fidelity and accuracy. Here, the authors devise a novel multiple displacement amplification method called TruePrime that is based in Thermus thermophilusPrimPol and Phi29 DNA polymerase, and ...
Ángel J. Picher, Bettina Budeus, Oliver Wafzig, Carola Krüger, Sara García-Gómez, María I. Martínez-Jiménez, Alberto Díaz-Talavera, Daniela Weber, Luis Blanco, Armin Schneider   +9 more
doaj   +1 more source

Enzymatic Synthesis of Enantiopure (R)‐Citronellal from Geraniol via a Short‐Chain Dehydrogenase and Ene Reductase

Advanced Synthesis &Catalysis, Volume 367, Issue 14, July 29, 2025.
A synthetic cascade with two consecutive biocatalytic steps consisting of a short‐chain alcohol dehydrogenase and an ene reductase in a biphasic system provides (R)‐citronellal with >90% conversion and >99% enantiomeric excess from inexpensive geraniol. The development of synthetic routes to produce enantiopure (R)‐citronellal as a key intermediate for
Claudia Ferrer‐Carbonell, Rocio Villa, Iris Viskaal, Diederik J. Opperman, Caroline E. Paul   +4 more
wiley   +1 more source

Mammalian Tolerance to Amino Acid Heterochirality

ChemBioChem, Volume 26, Issue 13, July 11, 2025.
Organisms preferentially use l‐amino acids (l‐AAs). However, de novo synthesized l‐AAs are in part converted to d‐AAs. The ribosome has a robust system to use l‐AAs for protein synthesis, whereas non‐ribosomal synthesis can also use d‐AAs. Furthermore, some AA residues can be post‐translationally isomerized through enzymatic or spontaneous processes ...
Sakiko Taniguchi, Kenichiro Adachi, Xuan Tran, Masataka Suzuki, Jumpei Sasabe   +4 more
wiley   +1 more source

Structural characterization of neutral and acidic glycolipids from Thermus thermophilus HB8. [PDF]

PLoS ONE, 2012
The structural characterization of glycolipids from Thermus thermophilus HB8 was performed in this study. Two neutral and one acidic glycolipids were extracted and purified by the modified TLC-blotting method, after which their chemical structures were ...
Yasuo Suda, Fumiaki Okazaki, Yushi Hasegawa, Seiji Adachi, Koichi Fukase, Susumu Kokubo, Seiki Kuramitsu, Shoichi Kusumoto   +7 more
doaj   +1 more source

In biomakromolekularen Komplexen können protonierte Glutamat‐ und Aspartat‐Seitenketten durch kurze und starke Wasserstoffbrückenbindungen zur Phosphodiestergruppenerkennung beitragen

Angewandte Chemie, Volume 137, Issue 27, July 1, 2025.
Die stark negativ geladenen Phosphodiestergruppen von Nukleinsäuren und zyklischen (Di‐)Nukleotiden interagieren häufig mit Aminosäuren, die polare oder positiv geladene Seitenketten enthalten. Aufgrund der Ladungsabstoßung mit ihren negativ geladenen Carboxylatgruppen enthaltenden Seitenketten gelten Glutamat und Aspartat generell als ungeeignet für ...
Konstantin Neißner, Elke Duchardt‐Ferner, Christoph Wiedemann, Julian Kraus, Ute A. Hellmich, Jens Wöhnert   +5 more
wiley   +1 more source

Thermus thermophilus Strains Active in Purine Nucleoside Synthesis

Molecules, 2009
Several strains of Thermus thermophilus were tested in order to detect purine nucleoside synthase activity using pyrimidine nucleosides as the sugar-donor and adenine or hypoxanthine as bases.
Marcos Almendros, José Berenguer Carlos, José-Vicente Sinisterra Gago   +2 more
doaj   +1 more source

Prokaryotic mechanosensitive channels mediate copper influx

Protein Science, Volume 34, Issue 7, July 2025.
Abstract Copper is an essential micronutrient in all kingdoms of life, requiring a meticulous balance between acquisition and toxic overload. While copper import in eukaryotes has been investigated extensively, few prokaryotic copper importers have been identified, leading to the notion that cytoplasmic copper uptake is unnecessary in prokaryotes. Here
Yara Ghnamah, Caitlin D. Palmer, Nurit Livnat‐Levanon, Moti Grupper, Amy C. Rosenzweig, Oded Lewinson   +5 more
wiley   +1 more source

Phosphate rock reduces the bioavailability of heavy metals by influencing the bacterial communities during aerobic composting

Journal of Integrative Agriculture, 2021
Available information on the microbial mechanisms associated with heavy metal (HM) passivation during co-composting amended with phosphate rock (PR) remains limited.
Hu CUI, Yang OU, Li-xia WANG, Bai-xing YAN, Ying-xin LI, Da-wei DING   +5 more
doaj  

Crystal structure of Thermus thermophilus methylenetetrahydrofolate dehydrogenase and determinants of thermostability.

PLoS ONE, 2020
The elucidation of mechanisms behind the thermostability of proteins is extremely important both from the theoretical and applied perspective. Here we report the crystal structure of methylenetetrahydrofolate dehydrogenase (MTHFD) from Thermus ...
Fernando Maiello, Gloria Gallo, Camila Coelho, Fernanda Sucharski, Leon Hardy, Martin Würtele   +5 more
doaj   +1 more source

Lyophilised Reverse Transcriptase and Polymerase for Point‐of‐Care Diagnostics of SARS‐CoV‐2

Microbial Biotechnology, Volume 18, Issue 7, July 2025.
Design, expression and purification of Klentaq polymerase, Taq polymerase and MMLV reverse transcriptase to prepare a custom‐made amplification mix. To enable cold‐free storage, the amplification mix was lyophilised. The prepared lyophilised amplification mix was used on a point of care device to efficiently detect SARS‐CoV‐2 RNA.
Natalie Mutter, Barbara Posch, Yasmin Gillitschka, Nadezhda Kataeva, Birgit Michalek, Daniel Lager, Filip Staniszewski, Alexandra Schilder, Lidiya Osinkina, Maximilian Westenthanner, Joachim Stehr, Federico Buersgens, Johannes R. Peham   +12 more
wiley   +1 more source