Complete Genome Sequence of Thermus aquaticus Y51MC23. [PDF]
PLoS ONE, 2015 Thermus aquaticus Y51MC23 was isolated from a boiling spring in the Lower Geyser Basin of Yellowstone National Park. Remarkably, this T. aquaticus strain is able to grow anaerobically and produces multiple morphological forms. Y51MC23 is a Gram-negative,
Phillip J Brumm +7 more
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A robust strategy for overexpression of DNA polymerase from Thermus aquaticus using an IPTG-independent autoinduction system in a benchtop bioreactor [PDF]
Scientific ReportsThe DNA polymerase derived from Thermus aquaticus is the most widely utilized among various DNA polymerases, indicating its significant economic importance. Consequently, efforts to achieve a substantial yield of Taq DNA polymerase (Taq-pol) are ongoing.
Fina Amreta Laksmi +11 more
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Crystal structure of a Thermus aquaticus diversity-generating retroelement variable protein. [PDF]
PLoS ONE, 2019 Diversity-generating retroelements (DGRs) are widely distributed in bacteria, archaea, and microbial viruses, and bring about unparalleled levels of sequence variation in target proteins.
Sumit Handa +2 more
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Variants of a Thermus aquaticus DNA polymerase with increased selectivity for applications in allele- and methylation-specific amplification. [PDF]
PLoS ONE, 2014 The selectivity of DNA polymerases is crucial for many applications. For example, high discrimination between the extension of matched versus mismatched primer termini is desired for the detection of a single nucleotide variation at a particular locus ...
Matthias Drum +4 more
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Inhibitory Effect of Bridged Nucleosides on Thermus aquaticus DNA Polymerase and Insight into the Binding Interactions. [PDF]
PLoS ONE, 2016 Modified nucleosides have the potential to inhibit DNA polymerases for the treatment of viral infections and cancer. With the hope of developing potent drug candidates by the modification of the 2',4'-position of the ribose with the inclusion of a bridge,
Sung-Kun Kim +5 more
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Modified 'one amino acid-one codon' engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase. [PDF]
Microb Cell Fact, 2014 BACKGROUND: An industrial approach to protein production demands maximization of cloned gene expression, balanced with the recombinant host’s viability.
Zylicz-Stachula A +4 more
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Distinct functions of regions 1.1 and 1.2 of RNA polymerase σ subunits from Escherichia coli and Thermus aquaticus in transcription initiation. [PDF]
J Biol Chem, 2012 Background: RNA polymerases (RNAPs) from Thermus aquaticus and Escherichia coli differ in many aspects of transcription initiation. Results: Regions 1.1 and 1.2 of the σ subunit determine instability and cold sensitivity of promoter complexes of T ...
Miropolskaya N +5 more
europepmc +4 more sources
Incorporation of N7-Platinated Guanines into Thermus Aquaticus (Taq) DNA Polymerase: Atomistic Insights from Molecular Dynamics Simulations. [PDF]
Int J Mol Sci, 2023 In this work, we elucidated some key aspects of the mechanism of action of the cisplatin anticancer drug, cis-[Pt(NH3)2Cl2], involving direct interactions with free nucleotides.
De Castro F +5 more
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Identification of Thermus aquaticus DNA polymerase variants with increased mismatch discrimination and reverse transcriptase activity from a smart enzyme mutant library. [PDF]
Sci Rep, 2019 DNA polymerases the key enzymes for several biotechnological applications. Obviously, nature has not evolved these enzymes to be compatible with applications in biotechnology. Thus, engineering of a natural scaffold of DNA polymerases may lead to enzymes
Raghunathan G, Marx A.
europepmc +2 more sources
The third restriction-modification system from Thermus aquaticus YT-1: solving the riddle of two TaqII specificities. [PDF]
Nucleic Acids Res, 2017 Two restriction–modification systems have been previously discovered in Thermus aquaticus YT-1. TaqI is a 263-amino acid (aa) Type IIP restriction enzyme that recognizes and cleaves within the symmetric sequence 5′-TCGA-3′.
Skowron PM +13 more
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