Die erste dreidimensionale Struktur einer Glycosyltransferase,die die Bildung großer zyklischer Amylose katalysiert [PDF]
, 2001 Titelblatt Einleitung 1.1 Polysaccharid 1.2 Metabolismus der Stärke 1.3 Die Amylomaltase 1.4 Industrielle Anwendung der Stärke-produzierenden Enzyme und deren Produkte 1.5 Zielsetzung Material und Methoden 2.1 Materialien 2.2 Biochemische ...
Przylas, Ingo
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Thermus aquaticus Brock & Freeze 1969, sp. n.
, 1969 Published as part of Brock, Thomas D. & Freeze, Hudson, 1969, Thermus aquaticus gen. n. and sp. n., a Nonsporulating Extreme Thermophile, pp. 289-297 in Journal of Bacteriology 98 (1) on pages 295-297, DOI: 10.1128/jb.98.1.289-297.1969, http://zenodo.org/record ...
Brock, Thomas D., Freeze, Hudson
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, 2008 Termofilní enzymy se tvoří v organismech žijících při teplotách 60-80°C, v některých případech i vyšších a jsou za těchto teplot aktivní a stabilní. Tato práce se zabývá výskytem termofilních a hypertermofilních organismů, způsoby jejich adaptace na ...
Kuchtová, Ludmila
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A simplified protocol for producing Taq DNA polymerase in biology laboratory
Research in Molecular Medicine, 2013 Background: Taq DNA polymerase is a very important enzyme for molecular biological studies such as DNA amplification and DNA sequencing by the PCR. It is a standard enzyme that is used in 90% of molecular biology labs today.
Touraj Farazmandfar +5 more
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Purification and characterization of a repressible alkaline phosphatase from Thermus aquaticus.
Journal of Biological Chemistry, 1976 A repressible alkaline phosphatase has been isolated from the extreme bacterial thermophile, Thermus aquaticus. The enzyme can be derepressed more than 1,000-fold by starving the cells for phosphate. In derepressed cells, nearly 6% of the total protein in a cell-free enzyme preparation is alkaline phosphatase.
M F Yeh, J M Trela
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Single-Cell-Genomics-Facilitated Read Binning of Candidate Phylum EM19 Genomes from Geothermal Spring Metagenomes [PDF]
, 2015 The vast majority of microbial life remains uncatalogued due to the inability to cultivate these organisms in the laboratory. This “microbial dark matter” represents a substantial portion of the tree of life and of the populations that contribute to ...
Becraft, Eric D. +10 more
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Crystal structure of a thermostable Bacillus DNA polymerase l large fragment at 2.1 Å resolution [PDF]
, 1997 Background: The study of DNA polymerases in the Pol l family is central to the understanding of DNA replication and repair. DNA polymerases are used in many molecular biology techniques, including PCR, which require a thermostable polymerase. In order to
Basehore, Scott L +6 more
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Isolation of strains ofThermus aquaticusfrom the Australian Artesian Basin and a simple and rapid procedure for the preparation of their plasmids [PDF]
, 1991 Stuart E. Denman +2 more
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Interaction of CarD with RNA polymerase mediates Mycobacterium tuberculosis viability, rifampin resistance, and pathogenesis [PDF]
, 2012 Mycobacterium tuberculosis infection continues to cause substantial human suffering. New chemotherapeutic strategies, which require insight into the pathways essential for M. tuberculosis pathogenesis, are imperative.
Campbell, Elizabeth A. +6 more
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BMC BiotechnologyThermostable DNA polymerases, such as Taq isolated from the thermophilic bacterium Thermus aquaticus, enable one-pot exponential DNA amplification known as polymerase chain reaction (PCR). However, properties other than thermostability - such as fidelity,
Cristina Hernández-Rollán +4 more
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