Results 11 to 20 of about 4,002 (146)

From discovery to innovation in physiological research. [PDF]

open access: yesExp Physiol
Experimental Physiology, Volume 110, Issue 3, Page 355-357, 1 March 2025.
Zacho M.
europepmc   +2 more sources

A Single Amino Acid Change to Taq DNA Polymerase Enables Faster PCR, Reverse Transcription and Strand-Displacement

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
A change of an aspartic acid to asparagine of Taq (Thermus aquaticus) DNA polymerase is a gain of function mutation that supports faster PCR: the extension times for PCR amplification can be 2–3 times shorter.
Wayne M. Barnes   +3 more
doaj   +1 more source

Measuring the absolute abundance of the microbiome by adding yeast containing 16S rRNA gene from a hyperthermophile

open access: yesMicrobiologyOpen, 2021
High‐throughput sequencing (HTS) of 16S rRNA gene amplicons provides compositional information regarding the microbial community, but not the absolute abundance of the bacteria.
Ju Yeong Kim   +6 more
doaj   +1 more source

Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1

open access: yesMicrobiologyOpen, 2021
Several native and engineered heat‐stable DNA polymerases from a variety of sources are used as powerful tools in different molecular techniques, including polymerase chain reaction, medical diagnostics, DNA sequencing, biological diversity assessments ...
Ani Saghatelyan   +3 more
doaj   +1 more source

Thermophilic bacteria colony growth and its consequences in the food industry

open access: yesCzech Journal of Food Sciences, 2004
The growth kinetics of thermophilic bacteria Bacillus acidocaldarius (CCM 3497), Bacillus stearothermophilus (CCMI 237), Thermus aquaticus (CCM 3488), Thermus ruber (CCM 4212), and Thermus species (CCM 4199) on solid media were studied in the temperature
K. Melzoch   +4 more
doaj   +1 more source

Structure of the GDP-bound state of the SRP GTPase FlhF. [PDF]

open access: yesActa Crystallogr F Struct Biol Commun
This study presents the X‐ray structure of FlhF in its GDP‐bound state at a resolution of 2.28 Å, exhibiting the classical N‐ and G‐domain fold. Comparative analysis with GTP‐loaded FlhF elucidates the conformational changes associated with GTP hydrolysis.The GTPase FlhF, a signal recognition particle (SRP)‐type enzyme, is pivotal for spatial–numerical
Dornes A, Mais CN, Bange G.
europepmc   +2 more sources

Impact of disturbance and dietary shift on gastrointestinal bacterial community and its invertebrate host system

open access: yesMolecular Ecology, Volume 32, Issue 23, Page 6631-6643, December 2023., 2023
Abstract The gut microbiome is one of the most important sites of host–microbe interactions, however, mechanisms governing the responses of host‐associated microbes to changing environmental conditions are poorly understood. To address this, we investigated individual and combined effects of dietary changes and increase in salinity (from freshwater to ...
Carmen Kivistik   +4 more
wiley   +1 more source

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