Growth models of Thermus aquaticus and Thermus scotoductus
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2012 Members of the genus Thermus family are signifiant producers of secondary metabolites, which are very commonly used in industry. Beside the productivity, it is also important to study the growth curve of each strain.
Libor Babák +2 more
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Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase. [PDF]
Sci Rep, 2019 We developed a novel and convenient method for rapidly identifying CRISPR/Cas9-based genome-edited biallelic knockout (KO) cells/individuals carrying insertions or deletions of a few nucleotides (indels) by performing PCR on genomic DNA samples under ...
Sakurai T +5 more
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Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus [PDF]
Journal of Bacteriology, 1976 A stable deoxyribonucleic acid (DNA) polymerase (EC 2.7.7.7) with a temperature optimum of 80 degrees C has been purified from the extreme thermophile Thermus aquaticus. The enzyme is free from phosphomonoesterase, phosphodiesterase and single-stranded exonuclease activities.
A J Chien, D. Edgar, John M. Trela
semanticscholar +5 more sources
Highly conserved salt bridge stabilizes a proteinase K subfamily enzyme, Aqualysin I, from Thermus aquaticus YT-1. [PDF]
AMB Express, 2014 The proteinase K subfamily enzymes, thermophilic Aqualysin I (AQN) from Thermus aquaticus YT-1 and psychrophilic serine protease (VPR) from Vibrio sp. PA-44, have six and seven salt bridges, respectively.
Sakaguchi M +6 more
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RNA polymerase-promoter interactions determining different stability of the Escherichia coli and Thermus aquaticus transcription initiation complexes. [PDF]
Nucleic Acids Res, 2012 Transcription initiation complexes formed by bacterial RNA polymerases (RNAPs) exhibit dramatic species-specific differences in stability, leading to different strategies of transcription regulation.
Mekler V +4 more
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High-yield cycloamylose production from sweet potato starch using Pseudomonas isoamylase and Thermus aquaticus 4-α-glucanotransferase. [PDF]
Food Sci Biotechnol, 2016 Chu S +6 more
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Conformational dynamics of Thermus aquaticus DNA polymerase I during catalysis. [PDF]
J Mol Biol, 2014 Xu C, Maxwell BA, Suo Z.
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Engineering of novel DNA polymerase variants for single enzyme quantitative multiplex reverse transcription-PCR [PDF]
Scientific ReportsReverse transcription polymerase chain reaction (RT-PCR) has evolved as a widely used approach in biotechnology and molecular diagnostics. It represents a powerful tool for amplifying and analysing RNA molecules and has therefore found widespread ...
Luisa B. Huber +4 more
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Thermostable Aldolase from Thermus aquaticus [PDF]
Journal of Bacteriology, 1970 Data are presented on the purification and properties of the thermostable fructose-1,6-diphosphate aldolase of Thermus aquaticus , a nonsporulating, extreme thermophile. The enzyme shows little activity at temperatures below 60 C and optimal activity at about 95 C.
Hudson H. Freeze, Thomas D. Brock
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Protein turnover in the extreme thermophile Thermus aquaticus [PDF]
Journal of Bacteriology, 1979 Protein turnover in the extreme bacterial thermophile Thermus aquaticus was examined in exponential cultures at 75 degrees C. The relative amount of [3H]leucine incorporated into trichloroacetic acid-insoluble material was stable in pulse-chase experiments assayed over 2.5 h.
T Kenkel, John M. Trela
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