Results 201 to 210 of about 14,614 (212)
Some of the next articles are maybe not open access.
Preparation and Characterization of Recombinant Thermus aquaticus RNA Polymerase
2003Publisher Summary This chapter reviews the overproduced thermus aquaticus (Taq) RNAP subunits that are assembled in functional RNAP in vitro and in vivo when coexpressed in E. coli. Binding of the core enzyme, consists of the five core subunits—β´, β, a dimer of identical α subunits, and the ω subunit.
Konstantin D. Kuznedelov +2 more
openaire +3 more sources
Environmental Science & Technology, 2001
Thermus aquaticus and Thermus thermophilus, common inhabitants of terrestrial hot springs and thermally polluted domestic and industrial waters, have been found to rapidly oxidize arsenite to arsenate. Field investigations at a hot spring in Yellowstone National Park revealed conserved total arsenic transport and rapid arsenite oxidation occurring ...
Jillian F. Banfield +4 more
openaire +2 more sources
Thermus aquaticus and Thermus thermophilus, common inhabitants of terrestrial hot springs and thermally polluted domestic and industrial waters, have been found to rapidly oxidize arsenite to arsenate. Field investigations at a hot spring in Yellowstone National Park revealed conserved total arsenic transport and rapid arsenite oxidation occurring ...
Jillian F. Banfield +4 more
openaire +2 more sources
Electron image analysis of ribosomal subunits from Thermus aquaticus
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1992Electron micrographs of ribosomal subunits from the thermophilic bacterium Thermus aquaticus were analysed using multivariate statistical analysis and characteristic views constructed to reproducible spatial resolutions ranging from 1.9 to 3.6 nm. These views were comparable to morphological classes of Escherichia coli ribosomal subunits, albeit with ...
George Harauz +2 more
openaire +3 more sources
Efficient Separation of Thermus aquaticus EF-Tu Functional Complexes
Biochemical and Biophysical Research Communications, 2001A new method for fast separation of the main functional complexes of the elongation factor Tu from Thermus aquaticus has been developed. Binary complexes EF-Tu * GDP and EF-Tu * GDPNP as well as the ternary complex EF-Tu * GDPNP * Leu approximately tRNA were separated from each other by means of HPLC on a hydrophobic sorbent TSK-Gel Phenyl 5PW in a ...
Stepanov, V.G., Nyborg, J.
openaire +4 more sources
Structure and flexibility of the thermophilic cold-shock protein of Thermus aquaticus
Biochemical and Biophysical Research Communications, 2014The thermophilic bacterium Thermus aquaticus is a well-known source of Taq polymerase. Here, we studied the structure and dynamics of the T. aquaticus cold-shock protein (Ta-Csp) to better understand its thermostability using NMR spectroscopy. We found that Ta-Csp has a five-stranded β-barrel structure with five salt bridges which are important for ...
Bonghwan Jin +2 more
openaire +3 more sources
Inactivation of the 5′-3′ exonuclease of Thermus aquaticus DNA polymerase
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1995The gene for Thermus aquaticus (Taq) DNA polymerase enzyme (Taq Pol I) was mutagenized and sixty-two candidate clones were screened for enzyme activity. Two of the clones expressed enzymes (*Taq-3 and *Taq-5) that showed very reduced 5'-3' exonuclease activity and normal DNA polymerase activity.
Robb E. Moses +2 more
openaire +2 more sources
GroEL-like Protein Complex of Thermophilic Bacterium Thermus aquaticus
Biochemical and Biophysical Research Communications, 1993GroEL-like particles of Thermus aquaticus are homo-oligomeric complexes of two stacked seven member rings, sedimenting in the gradient at 20S. The apparent molecular mass of the native particles is 820,000 (+/- 30,000). The protein complex is composed with one polypeptide of M(r) 59,000.
Karel Mikulík, Oldrich Benada
openaire +3 more sources
Ireland — Biosensor from Thermus aquaticus
Biosensors and Bioelectronics, 1995openaire +2 more sources
Fatty acid composition of Thermus aquaticus at different growth temperatures
Archiv f�r Mikrobiologie, 1970Carol Volkmann +5 more
openaire +3 more sources