Results 21 to 30 of about 14,614 (212)

A Single Amino Acid Change to Taq DNA Polymerase Enables Faster PCR, Reverse Transcription and Strand-Displacement

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
A change of an aspartic acid to asparagine of Taq (Thermus aquaticus) DNA polymerase is a gain of function mutation that supports faster PCR: the extension times for PCR amplification can be 2–3 times shorter.
Wayne M. Barnes   +3 more
doaj   +1 more source

Measuring the absolute abundance of the microbiome by adding yeast containing 16S rRNA gene from a hyperthermophile

open access: yesMicrobiologyOpen, 2021
High‐throughput sequencing (HTS) of 16S rRNA gene amplicons provides compositional information regarding the microbial community, but not the absolute abundance of the bacteria.
Ju Yeong Kim   +6 more
doaj   +1 more source

Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1

open access: yesMicrobiologyOpen, 2021
Several native and engineered heat‐stable DNA polymerases from a variety of sources are used as powerful tools in different molecular techniques, including polymerase chain reaction, medical diagnostics, DNA sequencing, biological diversity assessments ...
Ani Saghatelyan   +3 more
doaj   +1 more source

Impact of disturbance and dietary shift on gastrointestinal bacterial community and its invertebrate host system

open access: yesMolecular Ecology, Volume 32, Issue 23, Page 6631-6643, December 2023., 2023
Abstract The gut microbiome is one of the most important sites of host–microbe interactions, however, mechanisms governing the responses of host‐associated microbes to changing environmental conditions are poorly understood. To address this, we investigated individual and combined effects of dietary changes and increase in salinity (from freshwater to ...
Carmen Kivistik   +4 more
wiley   +1 more source

Fine Structure of Thermus aquaticus , an Extreme Thermophile [PDF]

open access: yesJournal of Bacteriology, 1970
Electron microscopic studies using thin sections revealed that Thermus aquaticus has a structure similar to that of most other gram-negative bacteria. The cell envelope is tripartite: plasma membrane, thin middle layer, and a thicker and irregular outer layer.
Mercedes R. Edwards   +3 more
openaire   +2 more sources

Sterically Enhanced Control of Enzyme‐Assisted DNA Assembly**

open access: yesChemBioChem, Volume 24, Issue 22, November 16, 2023., 2023
The sterically controlled, nuclease enhanced DNA assembly technique successfully assembles DNA structures containing multiple capture probes. Short (60 bp) DNA stands, with probes attached, are assembled with larger (1 kb) strands, overcoming the limitations of Gibson assembly, and offering a multiplex diagnostic tool.
Oliver J. Irving   +5 more
wiley   +1 more source

Biochemical characterisation of a novel broad pH spectrum subtilisin from Fictibacillus arsenicus DSM 15822T

open access: yesFEBS Open Bio, Volume 13, Issue 11, Page 2035-2046, November 2023., 2023
Microbial subtilisins, particularly from the Bacillaceae family, have significant potential for biotechnological applications in the enzyme market. In this study, the gene of the subtilisin SPFA from Fictibacillus arsenicus was cloned and expressed in Bacillus subtilis DB104.
Fabian Falkenberg   +4 more
wiley   +1 more source

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