Results 21 to 30 of about 4,002 (146)
Sterically Enhanced Control of Enzyme‐Assisted DNA Assembly**
ChemBioChem, Volume 24, Issue 22, November 16, 2023., 2023 The sterically controlled, nuclease enhanced DNA assembly technique successfully assembles DNA structures containing multiple capture probes. Short (60 bp) DNA stands, with probes attached, are assembled with larger (1 kb) strands, overcoming the limitations of Gibson assembly, and offering a multiplex diagnostic tool.
Oliver J. Irving +5 more
wiley +1 more source
FEBS Open Bio, Volume 13, Issue 11, Page 2035-2046, November 2023., 2023 Microbial subtilisins, particularly from the Bacillaceae family, have significant potential for biotechnological applications in the enzyme market. In this study, the gene of the subtilisin SPFA from Fictibacillus arsenicus was cloned and expressed in Bacillus subtilis DB104.
Fabian Falkenberg +4 more
wiley +1 more source
BioTechniques, 2000 A new method to produce hot-start conditions in PCR is described. Short double-stranded DNA fragments were found to inhibit the activity of DNA polymerases from Thermus aquaticus and Thermus flavus.
Peter Kainz +2 more
doaj +1 more source
DNA Polimerase sebagai Model Kajian Biomolekul Enzim Termostabil Isolat Lokal
Jurnal Kimia Sains dan Aplikasi, 2000 Ekstremozim merupakan bahan kajian penelitian yang banyak dilakukan dalam dekade terakhir. Enzim ini merupakan biokatalis yang sangat efektif digunakan dalam proses industri.
Agustina L. N. Aminin +2 more
doaj +1 more source
A ring-like nucleoid is not necessary for radioresistance in the
BMC Microbiology, 2005 Background Transmission electron microscopy images of Deinococcus radiodurans R1 suggest that the nucleoid of this species exists as a "ring-like" body, and have led to speculation that this structure contributes to the radioresistance of the species ...
Battista John R, Zimmerman Julie M
doaj +1 more source
Frontiers in Microbiology, 2016 A filamentous bacteriophage, φOH3, was isolated from hot spring sediment in Obama hot spring in Japan with the hyperthermophilic bacterium Thermus thermophilus HB8 as its host.
Yuko eNagayoshi +10 more
doaj +1 more source
Variable termination sites of DNA polymerases encountering a DNA-protein cross-link. [PDF]
PLoS ONE, 2018 DNA-protein cross-links (DPCs) are important DNA lesions induced by endogenous crosslinking agents such as formaldehyde or acetaldehyde, as well as ionizing radiation, cancer chemotherapeutic drugs, and abortive action of some enzymes.
Anna V Yudkina +2 more
doaj +1 more source
Frontiers in Microbiology, 2014 DNA polymerases are widely used for DNA manipulation in vitro, including DNA cloning, sequencing, DNA labeling, mutagenesis, and other experiments. Thermostable DNA polymerases are especially useful and became quite valuable after the development of PCR ...
Takeshi eYamagami +4 more
doaj +1 more source
CarD uses a minor groove wedge mechanism to stabilize the RNA polymerase open promoter complex
eLife, 2015 A key point to regulate gene expression is at transcription initiation, and activators play a major role. CarD, an essential activator in Mycobacterium tuberculosis, is found in many bacteria, including Thermus species, but absent in Escherichia coli. To
Brian Bae +5 more
doaj +1 more source
Structure of a bacterial RNA polymerase holoenzyme open promoter complex
eLife, 2015 Initiation of transcription is a primary means for controlling gene expression. In bacteria, the RNA polymerase (RNAP) holoenzyme binds and unwinds promoter DNA, forming the transcription bubble of the open promoter complex (RPo).
Brian Bae +4 more
doaj +1 more source