Results 21 to 30 of about 14,614 (212)
Use of random and saturation mutageneses to improve the properties of Thermus aquaticus amylomaltase for efficient production of cycloamyloses. [PDF]
Fujii K+6 more
europepmc +2 more sources
Biochemical and structural characterization of the GTP-preferring succinyl-CoA synthetase from Thermus aquaticus. [PDF]
Joyce MA+3 more
europepmc +3 more sources
A change of an aspartic acid to asparagine of Taq (Thermus aquaticus) DNA polymerase is a gain of function mutation that supports faster PCR: the extension times for PCR amplification can be 2–3 times shorter.
Wayne M. Barnes+3 more
doaj +1 more source
High‐throughput sequencing (HTS) of 16S rRNA gene amplicons provides compositional information regarding the microbial community, but not the absolute abundance of the bacteria.
Ju Yeong Kim+6 more
doaj +1 more source
Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1
Several native and engineered heat‐stable DNA polymerases from a variety of sources are used as powerful tools in different molecular techniques, including polymerase chain reaction, medical diagnostics, DNA sequencing, biological diversity assessments ...
Ani Saghatelyan+3 more
doaj +1 more source
A novel exopolysaccharide from the biofilm of Thermus aquaticus YT-1 induces the immune response through Toll-like receptor 2. [PDF]
Lin MH+9 more
europepmc +3 more sources
Abstract The gut microbiome is one of the most important sites of host–microbe interactions, however, mechanisms governing the responses of host‐associated microbes to changing environmental conditions are poorly understood. To address this, we investigated individual and combined effects of dietary changes and increase in salinity (from freshwater to ...
Carmen Kivistik+4 more
wiley +1 more source
Fine Structure of Thermus aquaticus , an Extreme Thermophile [PDF]
Electron microscopic studies using thin sections revealed that Thermus aquaticus has a structure similar to that of most other gram-negative bacteria. The cell envelope is tripartite: plasma membrane, thin middle layer, and a thicker and irregular outer layer.
Mercedes R. Edwards+3 more
openaire +2 more sources
Sterically Enhanced Control of Enzyme‐Assisted DNA Assembly**
The sterically controlled, nuclease enhanced DNA assembly technique successfully assembles DNA structures containing multiple capture probes. Short (60 bp) DNA stands, with probes attached, are assembled with larger (1 kb) strands, overcoming the limitations of Gibson assembly, and offering a multiplex diagnostic tool.
Oliver J. Irving+5 more
wiley +1 more source
Microbial subtilisins, particularly from the Bacillaceae family, have significant potential for biotechnological applications in the enzyme market. In this study, the gene of the subtilisin SPFA from Fictibacillus arsenicus was cloned and expressed in Bacillus subtilis DB104.
Fabian Falkenberg+4 more
wiley +1 more source