Results 31 to 40 of about 14,614 (212)
Plasmid-based lacZalpha assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase [PDF]
, 2009 The preparation of a gapped pUC18 derivative, containing the lacZalpha reporter gene in the single-stranded region, is described. Gapping is achieved by flanking the lacZalpha gene with sites for two related nicking endonucleases, enabling the excision ...
Bebenek +28 more
core +2 more sources
Thermophilic bacteria colony growth and its consequences in the food industry
Czech Journal of Food Sciences, 2004 The growth kinetics of thermophilic bacteria Bacillus acidocaldarius (CCM 3497), Bacillus stearothermophilus (CCMI 237), Thermus aquaticus (CCM 3488), Thermus ruber (CCM 4212), and Thermus species (CCM 4199) on solid media were studied in the temperature
K. Melzoch +4 more
doaj +1 more source
Brain Pathology, Volume 33, Issue 6, November 2023., 2023 The knockout‐validated antibody Y188 against APP (C‐terminus) can detect TBI‐induced pathology including axonal blebs in gray matter, and punctate and perivascular staining in white matter, but the widely used N‐terminal antibody 22C11 cannot. We also showed increased staining for endogenous biotin after TBI, which will be recognized by ABC when ...
Guoxiang Xiong +8 more
wiley +1 more source
Chemistry – A European Journal, Volume 29, Issue 36, June 27, 2023., 2023 Clostridium difficile frequently causes an infectious disease known worldwide as Clostridium difficile infection (CDI). An RNA‐cleaving fluorogenic DNAzyme was selected that does not recognize other species of bacteria but can be sensitively activated by diverse clinical strains of C. difficile.
Shuwen Qian +4 more
wiley +1 more source
Unconventional lateral gene transfer in extreme thermophilic bacteria [PDF]
, 2012 Conjugation and natural competence are two major mechanisms that explain the acquisition of foreign genes throughout bacterial evolution. In recent decades, several studies in model organisms have revealed in great detail the steps involved in such ...
Carlos Bricio +5 more
core +4 more sources
Chemie Ingenieur Technik, Volume 95, Issue 4, Page 518-530, April 2023., 2023 Dieser Übersichtsartikel beschäftigt sich mit wichtigen Entwicklungen auf dem Gebiet der DNA‐Methyltransferasen, wobei synthetische Analoga des Cofaktors S‐Adenosyl‐L‐methionin besondere Beachtung finden. Interessante aktuelle und potentielle Anwendungsmöglichkeiten in der Molekularbiologie und Biotechnologie werden diskutiert.
Nicolas V. Cornelissen +2 more
wiley +1 more source
BioTechniques, 2000 A new method to produce hot-start conditions in PCR is described. Short double-stranded DNA fragments were found to inhibit the activity of DNA polymerases from Thermus aquaticus and Thermus flavus.
Peter Kainz +2 more
doaj +1 more source
Effects of oxygen and methyl viologen on Thermus aquaticus [PDF]
Journal of Bacteriology, 1988 Under increased oxygen tensions, Thermus aquaticus exhibited a lag period in growth of 80 min, during which the specific activities of catalase, peroxidase, and superoxide dismutase increased. Methyl viologen increased the lag period, decreased the maximum population density, increased cell lysis, and induced catalase and superoxide dismutase.
openaire +3 more sources
A thermostable sequence-specific endonuclease from Thermus aquaticus. [PDF]
Proceedings of the National Academy of Sciences, 1977 A sequence-specific endonuclease, Taq I, of novel specificity has been partially purified from an extreme thermophile, Thermus aquaticus. The enzyme cleaves bacteriophage lambda DNA at many (greater than 30) sites and bacteriophage psiX174 RF DNA at 10 sites. The enzyme is active at temperatures up to 70 degrees.
J I Harris, C A Hutchinson rd, S Sato
openaire +3 more sources
DNA Polimerase sebagai Model Kajian Biomolekul Enzim Termostabil Isolat Lokal
Jurnal Kimia Sains dan Aplikasi, 2000 Ekstremozim merupakan bahan kajian penelitian yang banyak dilakukan dalam dekade terakhir. Enzim ini merupakan biokatalis yang sangat efektif digunakan dalam proses industri.
Agustina L. N. Aminin +2 more
doaj +1 more source