Results 31 to 40 of about 4,002 (146)

Use of a competitive probe in assay design for genotyping of the UGT1A1*28 microsatellite polymorphism by the smart amplification process

BioTechniques, 2007
A key feature of the smart amplification process version 2 (SMAP-2) is the ability to suppress mismatch amplification by using a unique asymmetric primer design and Thermus aquaticus MutS (Taq MutS).
Jun Watanabe, Yasumasa Mitani, Yuki Kawai, Takeshi Kikuchi, Yasushi Kogo, Atsuko Oguchi-Katayama, Hajime Kanamori, Kengo Usui, Masayoshi Itoh, Paul E. Cizdziel, Alexander Lezhava, Kenji Tatsumi, Yasushi Ichikawa, Shinji Togo, Hiroshi Shimada, Yoshihide Hayashizaki   +15 more
doaj   +1 more source

A simple and efficient method for extraction of Taq DNA polymerase

Electronic Journal of Biotechnology, 2015
Background: Thermostable DNA polymerase (Taq Pol Ι) from Thermus aquaticus has been widely used in PCR, which was usually extracted with Pluthero's method.
Sique Chen, Xiujuan Zheng, Hongrui Cao, Linghui Jiang, Fangqian Liu, Xinli Sun   +5 more
doaj   +1 more source

Advancements in DNA Tagging and Storage: Techniques, Applications, and Future Implications

WIREs Computational Molecular Science, Volume 15, Issue 4, July/August 2025.
Schematic comparison of DNA tagging and DNA storage technologies ‐ key features, applications, and workflow differences. ABSTRACT DNA‐based technologies for object authentication and data storage are becoming an interesting alternative to classic identification systems, yet their practical implementation faces fundamental technical and commercial ...
Adam Kuzdraliński, Marek Miśkiewicz, Hubert Szczerba, Wojciech Mazurczyk, Tomasz Ociepa, Michał Lechowski, Bogdan Księżopolski   +6 more
wiley   +1 more source

Lyophilised Reverse Transcriptase and Polymerase for Point‐of‐Care Diagnostics of SARS‐CoV‐2

Microbial Biotechnology, Volume 18, Issue 7, July 2025.
Design, expression and purification of Klentaq polymerase, Taq polymerase and MMLV reverse transcriptase to prepare a custom‐made amplification mix. To enable cold‐free storage, the amplification mix was lyophilised. The prepared lyophilised amplification mix was used on a point of care device to efficiently detect SARS‐CoV‐2 RNA.
Natalie Mutter, Barbara Posch, Yasmin Gillitschka, Nadezhda Kataeva, Birgit Michalek, Daniel Lager, Filip Staniszewski, Alexandra Schilder, Lidiya Osinkina, Maximilian Westenthanner, Joachim Stehr, Federico Buersgens, Johannes R. Peham   +12 more
wiley   +1 more source

Solid‐Phase Synthesis and Biological Evaluation of Peptides ADP‐Ribosylated at Histidine

Angewandte Chemie, Volume 136, Issue 4, January 22, 2024.
We report the preparation of ribofuranosylated Fmoc‐histidine building blocks via a Mukaiyama‐type glycosylation. These building blocks were used in solid‐phase peptide synthesis, followed by on‐resin pyrophosphate construction and deprotection to access peptides containing N(τ)‐ and N(π)‐ADP‐ribosylated histidine.
Hugo Minnee, Johannes G. M. Rack, Gijsbert A. van der Marel, Herman S. Overkleeft, Jeroen D. C. Codée, Ivan Ahel, Dmitri V. Filippov   +6 more
wiley   +2 more sources

Accuracy of replication in the polymerase chain reaction. Comparison between Thermotoga maritima DNA polymerase and Thermus aquaticus DNA polymerase

Brazilian Journal of Medical and Biological Research, 1998
For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that
R.S. Diaz, E.C. Sabino
doaj   +1 more source

Extremophilic Microorganisms as a Source of Emerging Enzymes for the Food Industry: A Review

Food Science &Nutrition, Volume 13, Issue 1, January 2025.
Enzymes such as amylases, lipases, proteases, xylanases, laccases, pullulanase, chitinases, pectinases, esterases, isomerases, and dehydrogenases could be derived from extremophilic organisms like thermophiles, psychrophiles, acidophiles, alkaliphiles, and halophiles. These enzymes or extremozymes are applicable in the production of safe foods (such as
Tolulope Joshua Ashaolu, Tanu Malik, Rakhi Soni, Miguel A. Prieto, Seid Mahdi Jafari   +4 more
wiley   +1 more source

Eine DNA‐Polymerase‐Variante erkennt den epigenetischen Marker 5‐Methylcytosin durch erhöhten Fehleinbau

Angewandte Chemie, Volume 136, Issue 48, November 25, 2024.
Entwicklung einer DNA‐Polymerase aus Thermus aquaticus, die methylierte von unmethylierter DNA durch einen erhöhten Fehleinbau gegenüber 5‐Methylcytosin (5 mC) während der DNA‐Synthese unterscheidet. Durch die Erzeugung von 5 mC‐abhängigen Mutationssignaturen im PCR‐Produkt ist das Enzym in der Lage, methylierte Stellen zu erkennen und ermöglicht den ...
Melanie Henkel, Alexander Fillbrunn, Virginie Marchand, Govindan Raghunathan, Michael R. Berthold, Yuri Motorin, Andreas Marx   +6 more
wiley   +1 more source

A 7-Deazaadenosylaziridine Cofactor for Sequence-Specific Labeling of DNA by the DNA Cytosine-C5 Methyltransferase M.HhaI

Molecules, 2015
DNA methyltransferases (MTases) catalyze the transfer of the activated methyl group of the cofactor S-adenosyl-l-methionine (AdoMet or SAM) to the exocyclic amino groups of adenine or cytosine or the C5 ring atom of cytosine within specific DNA sequences.
Falk Kunkel, Rudi Lurz, Elmar Weinhold
doaj   +1 more source

A DNA Polymerase Variant Senses the Epigenetic Marker 5‐Methylcytosine by Increased Misincorporation

Angewandte Chemie International Edition, Volume 63, Issue 48, November 25, 2024.
Engineering of a Thermus aquaticus DNA polymerase that distinguishes methylated from unmethylated DNA by increased misincorporation opposite 5‐methylcytosine (5 mC) during DNA synthesis. By generating 5 mC‐dependent mutation signatures in the PCR product, the enzyme is able to sense methylated sites and allows direct detection by reading elevated error
Melanie Henkel, Alexander Fillbrunn, Virginie Marchand, Govindan Raghunathan, Michael R. Berthold, Yuri Motorin, Andreas Marx   +6 more
wiley   +1 more source