Results 41 to 50 of about 24,101 (229)

Detection and verification of neurodegeneration after traumatic brain injury in the mouse: Immunohistochemical staining for amyloid precursor protein

Brain Pathology, Volume 33, Issue 6, November 2023., 2023
The knockout‐validated antibody Y188 against APP (C‐terminus) can detect TBI‐induced pathology including axonal blebs in gray matter, and punctate and perivascular staining in white matter, but the widely used N‐terminal antibody 22C11 cannot. We also showed increased staining for endogenous biotin after TBI, which will be recognized by ABC when ...
Guoxiang Xiong, Hannah Metheny, Kaitlin Hood, Ian Jean, Anthony M. Farrugia, Brian N. Johnson, Shanti R. Tummala, Noam A. Cohen, Akiva S. Cohen   +8 more
wiley   +1 more source

Specificity-Enhanced Hot-Start PCR: Addition of Double-Stranded DNA Fragments Adapted to the Annealing Temperature

BioTechniques, 2000
A new method to produce hot-start conditions in PCR is described. Short double-stranded DNA fragments were found to inhibit the activity of DNA polymerases from Thermus aquaticus and Thermus flavus.
Peter Kainz, Angela Schmiedlechner, Hans Bernd Strack   +2 more
doaj   +1 more source

Structural Evolution of Bacterial Polyphosphate Degradation Enzyme for Phosphorus Cycling. [PDF]

Adv Sci (Weinh)
This study demonstrates an interdomain linker‐based exopolyphosphatase (PPX) structural evolution in bacteria. The length of ɑ‐linker in PPX, which involves phosphate cycling, is varied among bacteria and has impacts on protein's conformation and quaternary structure, thus posing an impact on enzyme activity and thermostability. These results suggest a
Dai S, Wang B, Ye R, Zhang D, Xie Z, Yu N, Cai C, Huang C, Zhao J, Zhang F, Hua Y, Zhao Y, Zhou R, Tian B.   +13 more
europepmc   +2 more sources

Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]

, 1992
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke, AHN Hopman, AR Mitchell, C Huxley, C Lengauer, Christoph Lengauer, D Pinkel, ED Green, EW Jabs, HF Willard, HF Willard, HF Willard, HM Rosenberg, Ian Dunham, J Anastasi, J Meyne, JE Koch, JS Waye, JS Waye, JS Waye, L Manuelidis, L Manuelidis, M Klever, P Emmerich, PJ Poddighe, PR Langer, S Popp, T Cremer, T Cremer, T Cremer, T Imai, T Maniatis, Terry Featherstone, Thomas Cremer   +33 more
core   +1 more source

Effects of oxygen and methyl viologen on Thermus aquaticus [PDF]

Journal of Bacteriology, 1988
Under increased oxygen tensions, Thermus aquaticus exhibited a lag period in growth of 80 min, during which the specific activities of catalase, peroxidase, and superoxide dismutase increased. Methyl viologen increased the lag period, decreased the maximum population density, increased cell lysis, and induced catalase and superoxide dismutase.
openaire   +3 more sources

Microbial communities and arsenic biogeochemistry at the outflow of an alkaline sulfide-rich hot spring. [PDF]

, 2016
Alkaline sulfide-rich hot springs provide a unique environment for microbial community and arsenic (As) biogeochemistry. In this study, a representative alkaline sulfide-rich hot spring, Zimeiquan in the Tengchong geothermal area, was chosen to study ...
Dai, Xinyue, Jiang, Dawei, Jiang, Zhou, Li, Ping, Van Nostrand, Joy D, Wang, Yanhong, Wang, Yanxin, Zhang, Ping, Zhang, Rui, Zhou, Jizhong   +9 more
core   +1 more source

A thermostable sequence-specific endonuclease from Thermus aquaticus. [PDF]

Proceedings of the National Academy of Sciences, 1977
A sequence-specific endonuclease, Taq I, of novel specificity has been partially purified from an extreme thermophile, Thermus aquaticus. The enzyme cleaves bacteriophage lambda DNA at many (greater than 30) sites and bacteriophage psiX174 RF DNA at 10 sites. The enzyme is active at temperatures up to 70 degrees.
J I Harris, C A Hutchinson rd, S Sato
openaire   +3 more sources

DNA Polimerase sebagai Model Kajian Biomolekul Enzim Termostabil Isolat Lokal

Jurnal Kimia Sains dan Aplikasi, 2000
Ekstremozim merupakan bahan kajian penelitian yang banyak dilakukan dalam dekade terakhir. Enzim ini merupakan biokatalis yang sangat efektif digunakan dalam proses industri.
Agustina L. N. Aminin, Akhmaloka Akhmaloka, Hendro Pramono   +2 more
doaj   +1 more source

Origin of a folded repeat protein from an intrinsically disordered ancestor. [PDF]

, 2016
Repetitive proteins are thought to have arisen through the amplification of subdomain-sized peptides. Many of these originated in a non-repetitive context as cofactors of RNA-based replication and catalysis, and required the RNA to assume their active ...
Abe, Adams, Alva, Alva, Alva, Anantharaman, Apic, Aurora, Bansal, Bansal, Berman, Bernhardt, Biegert, Binz, Blundell, Boratyn, Broom, Bubunenko, Burton, Camacho, Chaudhuri, Chen, Coquille, Cortajarena, Cowtan, Crick, Crooks, D'Andrea, Das, Das, de Juan, Di Domenico, Dosztányi, Dosztányi, Doyle, Dunin-Horkawicz, Dyson, Eck, Eddy, Emsley, Finn, Forrer, Fox, Fox, Gilbert, Gorodkin, Grimsley, Habchi, Hsiao, Huson, Iwaya, Kabsch, Kabsch, Kajander, Kajava, Karpenahalli, Katibah, Keefe, Keiski, Kobe, Kohl, Koonin, Kopec, Krachler, Kumar, Kyrpides, Kyrpides, Lamb, Larkin, Laskowski, Lee, Linding, Lunelli, Lupas, Magliery, Main, Main, Main, McLachlan, McLachlan, Morcos, Mosavi, Murzin, Nguyen, Noble, Ohtani, Oldfield, Orengo, Park, Parmeggiani, Paterakis, Peng, Ponting, Ramarao, Ranea, Remmert, Romero, Rämisch, Sawyer, Schluenzen, Schrödinger,, Scott, Serasinghe, Shatsky, Sheldrick, Shen, Sikorski, Stumpp, Söding, Söding, Tobin, Vagin, Varadi, Wei, Wootton, Wootton, Zeytuni, Zeytuni, Zeytuni, Zhang   +119 more
core   +3 more sources

Plasmid-based lacZalpha assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase [PDF]

, 2009
The preparation of a gapped pUC18 derivative, containing the lacZalpha reporter gene in the single-stranded region, is described. Gapping is achieved by flanking the lacZalpha gene with sites for two related nicking endonucleases, enabling the excision ...
Bebenek, Bernard A. Connolly, Biles, Cline, Eckert, Evans, Firbank, Fortune, Fujii, Garcia-Diaz, Garg, Greagg, Hamilton, Hübscher, Karam, Krokan, Kunkel, Kunkel, Lindahl, Lundberg, Maor-Shoshani, McCulloch, Pavlov, Prakash, Shcherbakova, Stanislaw K. Jozwiakowski, Thomas, Tindall, Zheng   +28 more
core   +2 more sources