Results 41 to 50 of about 4,002 (146)

The One Health approach in food safety: Challenges and opportunities

Food Frontiers, Volume 5, Issue 5, Page 1837-1865, September 2024.
Abstract According to the World Health Organization, contaminated food causes approximately 600 million cases of illness and 420,000 associated deaths worldwide each year. In this scenario, food safety is a crucial task, which is based in the application of correct hygiene practices throughout the entire food production chain: from farm to table ...
Cristina Rodríguez‐Melcón, Carlos Alonso‐Calleja, Rosa Capita   +2 more
wiley   +1 more source

In planta imaging of pyridine nucleotides using second‐generation fluorescent protein biosensors

The Plant Journal, Volume 119, Issue 3, Page 1643-1658, August 2024.
SUMMARY Redox changes of pyridine nucleotides in cellular compartments are highly dynamic and their equilibria are under the influence of various reducing and oxidizing reactions. To obtain spatiotemporal data on pyridine nucleotides in living plant cells, typical biochemical approaches require cell destruction. To date, genetically encoded fluorescent
Shey‐Li Lim, Jinhong Liu, Gilles Dupouy, Gaurav Singh, Stéphanie Baudrey, Lang Yang, Jia Yi Zhong, Marie‐Edith Chabouté, Boon Leong Lim   +8 more
wiley   +1 more source

Structural Evolution of Bacterial Polyphosphate Degradation Enzyme for Phosphorus Cycling

Advanced Science, Volume 11, Issue 26, July 10, 2024.
This study demonstrates an interdomain linker‐based exopolyphosphatase (PPX) structural evolution in bacteria. The length of ɑ‐linker in PPX, which involves phosphate cycling, is varied among bacteria and has impacts on protein's conformation and quaternary structure, thus posing an impact on enzyme activity and thermostability. These results suggest a
Shang Dai, Binqiang Wang, Rui Ye, Dong Zhang, Zhenming Xie, Ning Yu, Chunhui Cai, Cheng Huang, Jie Zhao, Furong Zhang, Yuejin Hua, Ye Zhao, Ruhong Zhou, Bing Tian   +13 more
wiley   +1 more source

Atypical applications of transverse diffusion of laminar flow profiles methodology for in‐capillary reactions in capillary electrophoresis

Journal of Separation Science, Volume 47, Issue 13, July 2024.
Capillary electrophoresis (CE) is a powerful separation technique offering quick and efficient analyses in various fields of bioanalytical chemistry. It is characterized by many well‐known advantages, but one, which is perhaps the most important for this application field, is somewhat overlooked.
Taťána Bržezická, Lenka Kohútová, Zdeněk Glatz   +2 more
wiley   +1 more source

What is the role of microbial biotechnology and genetic engineering in medicine?

MicrobiologyOpen, Volume 13, Issue 2, April 2024.
Microbial biotechnology, the technological application of microorganisms, has been instrumental in producing significant natural bioactive products. These include antibiotics, antifungals, anticancer drugs, antiparasitics, antivirals, immunosuppressants, toxoid vaccines, and therapeutic enzymes.
Fernando Santos‐Beneit
wiley   +1 more source

Reversibly locked thionucleobase pairs in DNA to study base flipping enzymes

Beilstein Journal of Organic Chemistry, 2014
Covalently interstrand cross-linked DNA is an interesting tool to study DNA binding proteins that locally open up the DNA duplex by flipping single bases out of the DNA helix or melting whole stretches of base pairs to perform their function.
Christine Beuck, Elmar Weinhold
doaj   +1 more source

Attenuative effects of collagen peptide from milkfish (Chanos chanos) scales on ovariectomy‐induced osteoporosis

Food Science &Nutrition, Volume 12, Issue 1, Page 116-130, January 2024.
Collagen peptide from milkfish (Chanos chanos) may reduce RANKL‐induced osteoclast activity while promoting osteoblast synthesis, and therefore may act as a potential therapeutic agent for the prevention and control of osteoporosis. Abstract Osteoporosis is characterized by low bone mass, bone microarchitecture disruption, and collagen loss, leading to
Jiunn‐Jye Chuu, Jeng‐Wei Lu, Hung‐Ju Chang, You‐Hsiang Chu, Yi‐Jen Peng, Yi‐Jung Ho, Pei‐Hung Shen, Yu‐Shuan Cheng, Chia‐Hui Cheng, Yi‐Chien Liu, Chih‐Chien Wang   +10 more
wiley   +1 more source

Development of an Injectable DNA Vaccine Against Aeromonas hydrophila Infection Nanoencapsulated With Poly(Lactic‐Co‐Glycolic) Acid (PLGA) in Common Carp

Aquaculture Research, Volume 2024, Issue 1, 2024.
In this study, we developed an injectable DNA vaccine targeting the aopB gene of Aeromonas hydrophila, encapsulated within poly(lactic‐co‐glycolic acid) (PLGA) nanoparticles, for use in common carp. Juvenile common carp were divided into six groups with three replicates each.
Mojtaba Alishahi, Hoda Lababian, Hadi Heidari, Mohammad Reza Tabandeh, Mohammad Khosravi, Christyn Bailey   +5 more
wiley   +1 more source

Engineering of novel DNA polymerase variants for single enzyme quantitative multiplex reverse transcription-PCR

Scientific Reports
Reverse transcription polymerase chain reaction (RT-PCR) has evolved as a widely used approach in biotechnology and molecular diagnostics. It represents a powerful tool for amplifying and analysing RNA molecules and has therefore found widespread ...
Luisa B. Huber, Virginie Marchand, Melike Somtürk, Silke Müller, Andreas Marx   +4 more
doaj   +1 more source

A simplified protocol for producing Taq DNA polymerase in biology laboratory

Research in Molecular Medicine, 2013
Background: Taq DNA polymerase is a very important enzyme for molecular biological studies such as DNA amplification and DNA sequencing by the PCR. It is a standard enzyme that is used in 90% of molecular biology labs today.
Touraj Farazmandfar, Alireza Rafiei, Mohammad Bagher Hashemi-Sotehoh, Reza Valadan, Mohammad Alavi, Fatemeh Moradian   +5 more
doaj