Results 41 to 50 of about 14,614 (212)

A plasmid-based lacZα gene assay for DNA polymerase fidelity measurement [PDF]

, 2013
A significantly improved DNA polymerase fidelity assay, based on a gapped plasmid containing the lacZα reporter gene in a single-stranded region, is described.
Connolly, Bernard A, Jozwiakowski, Stanislaw K, Keith, Brian J   +2 more
core   +1 more source

Molecular basis for DarT ADP-ribosylation of a DNA base [PDF]

, 2021
ADP-ribosyltransferases use NAD+ to catalyse substrate ADP-ribosylation1, and thereby regulate cellular pathways or contribute to toxin-mediated pathogenicity of bacteria2–4.
Ahel, Ivan, Ariza, Antonio, Butler, Rachel, Claridge, Tim, Goh, Shan, Jankevicius, Gytis, Kendall, Sharon, Schuller, Marion, Stewart, Graham, Tromans-Coia, Callum   +9 more
core   +1 more source

A ring-like nucleoid is not necessary for radioresistance in the Deinococcaceae

BMC Microbiology, 2005
Background Transmission electron microscopy images of Deinococcus radiodurans R1 suggest that the nucleoid of this species exists as a "ring-like" body, and have led to speculation that this structure contributes to the radioresistance of the species ...
Battista John R, Zimmerman Julie M
doaj   +1 more source

Effect of Temperature on the Fatty Acid Composition of Thermus aquaticus [PDF]

Journal of Bacteriology, 1971
Thermus aquaticus contains four major fatty acids, iso-C 15 (28%), iso-C 16 (9%), normal-C 16 (13%), and iso-C 17 (48%), when grown at 70 C, as determined by gas chromatography and mass spectrometry. Small amounts of iso-C
Paul H. Ray, Paul H. Ray, Thomas D. Brock, Thomas D. Brock, David C. White, David C. White   +5 more
openaire   +3 more sources

Macromolecular mimicry in protein biosynthesis [PDF]

, 1997
Elongation factor Tu (EF-Tu) is a G-protein which, in its active GTP conformation, protects and carries aminoacylated tRNAs (aa-tRNAs) to the ribosome during protein biosynthesis.
Clark, Brian FC, Kjeldgaard, Morten, Nissen, Poul, Nyborg, Jens, Polekhina, Galina, Reshetnikova, Ludmila, Thirup, Søren   +6 more
core   +1 more source

Physiological properties and genome structure of the hyperthermophilic filamentous phage φOH3 which infects <i>Thermus thermophilu</i>s HB8

Frontiers in Microbiology, 2016
A filamentous bacteriophage, φOH3, was isolated from hot spring sediment in Obama hot spring in Japan with the hyperthermophilic bacterium Thermus thermophilus HB8 as its host.
Yuko eNagayoshi, Kenta eKumagae, Kazuki eMori, Kosuke eTashiro, Ayano eNakamura, Yasuhiro eFujino, Yasuaki eHiromasa, Takeo eIwamoto, Satoru eKuhara, Toshihisa eOhshima, Katsumi eDoi   +10 more
doaj   +1 more source

Variable termination sites of DNA polymerases encountering a DNA-protein cross-link. [PDF]

PLoS ONE, 2018
DNA-protein cross-links (DPCs) are important DNA lesions induced by endogenous crosslinking agents such as formaldehyde or acetaldehyde, as well as ionizing radiation, cancer chemotherapeutic drugs, and abortive action of some enzymes.
Anna V Yudkina, Antonina P Dvornikova, Dmitry O Zharkov   +2 more
doaj   +1 more source

Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]

, 1992
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke, AHN Hopman, AR Mitchell, C Huxley, C Lengauer, Christoph Lengauer, D Pinkel, ED Green, EW Jabs, HF Willard, HF Willard, HF Willard, HM Rosenberg, Ian Dunham, J Anastasi, J Meyne, JE Koch, JS Waye, JS Waye, JS Waye, L Manuelidis, L Manuelidis, M Klever, P Emmerich, PJ Poddighe, PR Langer, S Popp, T Cremer, T Cremer, T Cremer, T Imai, T Maniatis, Terry Featherstone, Thomas Cremer   +33 more
core   +1 more source

Mutant Taq DNA polymerases with improved elongation ability as a useful reagent for genetic engineering

Frontiers in Microbiology, 2014
DNA polymerases are widely used for DNA manipulation in vitro, including DNA cloning, sequencing, DNA labeling, mutagenesis, and other experiments. Thermostable DNA polymerases are especially useful and became quite valuable after the development of PCR ...
Takeshi eYamagami, Sonoko eIshino, Yutaka eKawarabayasi, Yutaka eKawarabayasi, Yoshizumi eIshino   +4 more
doaj   +1 more source

Origin of a folded repeat protein from an intrinsically disordered ancestor. [PDF]

, 2016
Repetitive proteins are thought to have arisen through the amplification of subdomain-sized peptides. Many of these originated in a non-repetitive context as cofactors of RNA-based replication and catalysis, and required the RNA to assume their active ...
Abe, Adams, Alva, Alva, Alva, Anantharaman, Apic, Aurora, Bansal, Bansal, Berman, Bernhardt, Biegert, Binz, Blundell, Boratyn, Broom, Bubunenko, Burton, Camacho, Chaudhuri, Chen, Coquille, Cortajarena, Cowtan, Crick, Crooks, D'Andrea, Das, Das, de Juan, Di Domenico, Dosztányi, Dosztányi, Doyle, Dunin-Horkawicz, Dyson, Eck, Eddy, Emsley, Finn, Forrer, Fox, Fox, Gilbert, Gorodkin, Grimsley, Habchi, Hsiao, Huson, Iwaya, Kabsch, Kabsch, Kajander, Kajava, Karpenahalli, Katibah, Keefe, Keiski, Kobe, Kohl, Koonin, Kopec, Krachler, Kumar, Kyrpides, Kyrpides, Lamb, Larkin, Laskowski, Lee, Linding, Lunelli, Lupas, Magliery, Main, Main, Main, McLachlan, McLachlan, Morcos, Mosavi, Murzin, Nguyen, Noble, Ohtani, Oldfield, Orengo, Park, Parmeggiani, Paterakis, Peng, Ponting, Ramarao, Ranea, Remmert, Romero, Rämisch, Sawyer, Schluenzen, Schrödinger,, Scott, Serasinghe, Shatsky, Sheldrick, Shen, Sikorski, Stumpp, Söding, Söding, Tobin, Vagin, Varadi, Wei, Wootton, Wootton, Zeytuni, Zeytuni, Zeytuni, Zhang   +119 more
core   +3 more sources