Results 51 to 60 of about 14,614 (212)
Transfer of transposon Tn916 from Bacillus subtilis to Thermus aquaticus [PDF]
FEMS Microbiology Letters, 1990 Broad host range conjugating transposon Tn916 has been introduced into the extreme thermophile Thermus by transposon transformation and transposition into the Bacillus subtilis chromosome followed by broth mating with Thermus aquaticus ATCC27634. Tetracycline resistant Thermus transconjugants were obtained at a frequency of 1.4 X 10(-7) per donor and 1.
Patrick Oriel, Sribir Sen
openaire +3 more sources
CarD uses a minor groove wedge mechanism to stabilize the RNA polymerase open promoter complex
eLife, 2015 A key point to regulate gene expression is at transcription initiation, and activators play a major role. CarD, an essential activator in Mycobacterium tuberculosis, is found in many bacteria, including Thermus species, but absent in Escherichia coli. To
Brian Bae +5 more
doaj +1 more source
Microbial communities and arsenic biogeochemistry at the outflow of an alkaline sulfide-rich hot spring. [PDF]
, 2016 Alkaline sulfide-rich hot springs provide a unique environment for microbial community and arsenic (As) biogeochemistry. In this study, a representative alkaline sulfide-rich hot spring, Zimeiquan in the Tengchong geothermal area, was chosen to study ...
Dai, Xinyue +9 more
core +1 more source
Structure of a bacterial RNA polymerase holoenzyme open promoter complex
eLife, 2015 Initiation of transcription is a primary means for controlling gene expression. In bacteria, the RNA polymerase (RNAP) holoenzyme binds and unwinds promoter DNA, forming the transcription bubble of the open promoter complex (RPo).
Brian Bae +4 more
doaj +1 more source
Unconventional lateral gene transfer in extreme thermophilic bacteria. [PDF]
, 2016 Conjugation and natural competence are two major mechanisms that explain the acquisition of foreign genes throughout bacterial evolution. In recent decades, several studies in model organisms have revealed in great detail the steps involved in such ...
Berenguer, José +4 more
core +1 more source
BioTechniques, 2007 A key feature of the smart amplification process version 2 (SMAP-2) is the ability to suppress mismatch amplification by using a unique asymmetric primer design and Thermus aquaticus MutS (Taq MutS).
Jun Watanabe +15 more
doaj +1 more source
A simple and efficient method for extraction of Taq DNA polymerase
Electronic Journal of Biotechnology, 2015 Background: Thermostable DNA polymerase (Taq Pol Ι) from Thermus aquaticus has been widely used in PCR, which was usually extracted with Pluthero's method.
Sique Chen +5 more
doaj +1 more source
Solid‐Phase Synthesis and Biological Evaluation of Peptides ADP‐Ribosylated at Histidine
Angewandte Chemie, Volume 136, Issue 4, January 22, 2024.We report the preparation of ribofuranosylated Fmoc‐histidine building blocks via a Mukaiyama‐type glycosylation. These building blocks were used in solid‐phase peptide synthesis, followed by on‐resin pyrophosphate construction and deprotection to access peptides containing N(τ)‐ and N(π)‐ADP‐ribosylated histidine.
Hugo Minnee +6 more
wiley +2 more sources
The upper temperature for life – where do we draw the line? [PDF]
, 2004 The newly isolated hyperthermophilic archaeal strain 121 grows slowly at 121 8C and even survives short periods at 130 8C. This is another organism that grows best at temperatures well in excess of 100 8C!
Cowan, Donald A.
core +1 more source
A structural role for the PHP domain in E. coli DNA polymerase III. [PDF]
, 2013 RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'.
Anaya, Jordan +7 more
core +4 more sources