Results 61 to 70 of about 14,614 (212)
Brazilian Journal of Medical and Biological Research, 1998 For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that
R.S. Diaz, E.C. Sabino
doaj +1 more source
Extremophilic Microorganisms as a Source of Emerging Enzymes for the Food Industry: A Review
Food Science &Nutrition, Volume 13, Issue 1, January 2025.Enzymes such as amylases, lipases, proteases, xylanases, laccases, pullulanase, chitinases, pectinases, esterases, isomerases, and dehydrogenases could be derived from extremophilic organisms like thermophiles, psychrophiles, acidophiles, alkaliphiles, and halophiles. These enzymes or extremozymes are applicable in the production of safe foods (such as
Tolulope Joshua Ashaolu +4 more
wiley +1 more source
Kinetic analysis of reverse transcriptase activity of bacterial family A DNA polymerases. [PDF]
, 2012 Some bacterial thermostable, wild-type or genetically engineered family A DNA polymerases have reverse transcriptase activity. However, difference in reverse transcriptase activities of family A DNA polymerases and retroviral reverse transcriptases (RTs)
Fujiwara, Shinsuke +4 more
core +1 more source
Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli [PDF]
Analytical Biochemistry, 1990 DNA polymerase from Thermus aquaticus has become a common reagent in molecular biology because of its utility in DNA amplification and DNA sequencing protocols. A simplified method is described here for isolating the recombinant Taq enzyme after overproduction in Escherichia coli.
Engelke, David R. +3 more
openaire +4 more sources
Angewandte Chemie, Volume 136, Issue 48, November 25, 2024.Entwicklung einer DNA‐Polymerase aus Thermus aquaticus, die methylierte von unmethylierter DNA durch einen erhöhten Fehleinbau gegenüber 5‐Methylcytosin (5 mC) während der DNA‐Synthese unterscheidet. Durch die Erzeugung von 5 mC‐abhängigen Mutationssignaturen im PCR‐Produkt ist das Enzym in der Lage, methylierte Stellen zu erkennen und ermöglicht den ...
Melanie Henkel +6 more
wiley +1 more source
Isolation, genome sequencing, assembly, annotation and Characterization of Thermus sp. CCB_US3_UF1 FROM Ulu Slim, Perak, Malaysia. [PDF]
, 2011 Thermus sp. CCB_US3_UF1, satu bacteria thermofilik telah Berjaya dipencilkan dari kolam air panas di Ulu Slim, Perak, Malaysia. Genomnya mengandungi 2,243,772 pasangan bes dan satu plasmid bersaiz 19,716 pasangan bes. Thermus sp.
Teh, Beng Soon
core
Biochemical and structural characterisation of membrane-containing icosahedral dsDNA bacteriophages infecting thermophilic Thermus thermophilus [PDF]
, 2008 Icosahedral dsDNA viruses isolated from hot springs and proposed to belong to the Tectiviridae family infect the Gram-negative thermophilic Thermus thermophilus bacterium. Seven such viruses were obtained from the Promega Corporation collection.
Bamford, D.H. +4 more
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A DNA Polymerase Variant Senses the Epigenetic Marker 5‐Methylcytosine by Increased Misincorporation
Angewandte Chemie International Edition, Volume 63, Issue 48, November 25, 2024.Engineering of a Thermus aquaticus DNA polymerase that distinguishes methylated from unmethylated DNA by increased misincorporation opposite 5‐methylcytosine (5 mC) during DNA synthesis. By generating 5 mC‐dependent mutation signatures in the PCR product, the enzyme is able to sense methylated sites and allows direct detection by reading elevated error
Melanie Henkel +6 more
wiley +1 more source
Caldolysin, a highly active protease from an extremely Thermophilic Bacterium [PDF]
, 1982 Proteases comprise a significant proportion of those proteins which have been subject to detailed characterisation (amino acid sequence and high resolution crystallographic analysis).
Cowan, Don A. +2 more
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Molecules, 2015 DNA methyltransferases (MTases) catalyze the transfer of the activated methyl group of the cofactor S-adenosyl-l-methionine (AdoMet or SAM) to the exocyclic amino groups of adenine or cytosine or the C5 ring atom of cytosine within specific DNA sequences.
Falk Kunkel, Rudi Lurz, Elmar Weinhold
doaj +1 more source