Results 61 to 70 of about 24,101 (229)

Biophysical analysis of Thermus aquaticus single-stranded DNA binding protein. [PDF]

Biophys J, 2008
Due to their involvement in processes such as DNA replication, repair, and recombination, bacterial single-stranded DNA binding (SSB) proteins are essential for the survival of the bacterial cell. Whereas most bacterial SSB proteins form homotetramers in solution, dimeric SSB proteins were recently discovered in the Thermus/Deinococcus group.
Witte G, Fedorov R, Curth U.
europepmc   +5 more sources

Structure of a bacterial RNA polymerase holoenzyme open promoter complex

eLife, 2015
Initiation of transcription is a primary means for controlling gene expression. In bacteria, the RNA polymerase (RNAP) holoenzyme binds and unwinds promoter DNA, forming the transcription bubble of the open promoter complex (RPo).
Brian Bae, Andrey Feklistov, Agnieszka Lass-Napiorkowska, Robert Landick, Seth A Darst   +4 more
doaj   +1 more source

Occurrence of phosphenolpyruvate carboxylase in the extremely thermophilic bacterium Thermus aquaticus [PDF]

Journal of Bacteriology, 1976
In the extreme thermophile Thermus aquaticus, phosphoenolpyruvate carboxylase catalyzes carbon dioxide fixation on the C3 metabolite phosphoenolpyruvate, producing oxaloacetate. In a moderately thermophilic Bacillus species this function is fulfilled by pyruvate carboyxlase.
T K Sundaram, G P Bridger
openaire   +3 more sources

Use of a competitive probe in assay design for genotyping of the UGT1A1*28 microsatellite polymorphism by the smart amplification process

BioTechniques, 2007
A key feature of the smart amplification process version 2 (SMAP-2) is the ability to suppress mismatch amplification by using a unique asymmetric primer design and Thermus aquaticus MutS (Taq MutS).
Jun Watanabe, Yasumasa Mitani, Yuki Kawai, Takeshi Kikuchi, Yasushi Kogo, Atsuko Oguchi-Katayama, Hajime Kanamori, Kengo Usui, Masayoshi Itoh, Paul E. Cizdziel, Alexander Lezhava, Kenji Tatsumi, Yasushi Ichikawa, Shinji Togo, Hiroshi Shimada, Yoshihide Hayashizaki   +15 more
doaj   +1 more source

Comparative chromosome band mapping in primates byin situ suppression hybridization of band specific DNA microlibraries [PDF]

, 1991
A DNA-library established from microdissected bands 8q23 to 8q24.1 of normal human chromosomes 8 (Lüdecke et al., 1989) was used as a probe for chromosomal in situ suppression (CISS-) hybridization to metaphase chromosomes of man and primates including ...
A. Jauch, B. Dutrillaux, B. Horsthemke, C. Lengauer, C. Lengauer, D. Pinkel, D. Pinkel, H. J. Lüdecke, H.J. Lüdecke, J. Wienberg, J. Wienberg, J.J. Yunis, M. Klever, M. Small, P. Lichter, P.A. Lalley, R. Stanyon, R. Stanyon, R. Stanyon, S.J. O'Brien, T. Cremer   +20 more
core   +1 more source

A simple and efficient method for extraction of Taq DNA polymerase

Electronic Journal of Biotechnology, 2015
Background: Thermostable DNA polymerase (Taq Pol Ι) from Thermus aquaticus has been widely used in PCR, which was usually extracted with Pluthero's method.
Sique Chen, Xiujuan Zheng, Hongrui Cao, Linghui Jiang, Fangqian Liu, Xinli Sun   +5 more
doaj   +1 more source

Isolation, genome sequencing, assembly, annotation and Characterization of Thermus sp. CCB_US3_UF1 FROM Ulu Slim, Perak, Malaysia. [PDF]

, 2011
Thermus sp. CCB_US3_UF1, satu bacteria thermofilik telah Berjaya dipencilkan dari kolam air panas di Ulu Slim, Perak, Malaysia. Genomnya mengandungi 2,243,772 pasangan bes dan satu plasmid bersaiz 19,716 pasangan bes. Thermus sp.
Teh, Beng Soon
core  

Structures of an apo and a binary complex of an evolved archeal b family DNA polymerase capable of synthesising highly cy-dye labelled DNA [PDF]

Thermophilic DNA polymerases of the polB family are of great importance in biotechnological applications including high-fidelity PCR. Of particular interest is the relative promiscuity of engineered versions of the exo- form of polymerases from the ...
Holliger, P., Leslie, A.G.W., Pinheiro, Vitor B., Wynne, S.A.   +3 more
core   +1 more source

Interaction of CarD with RNA polymerase mediates Mycobacterium tuberculosis viability, rifampin resistance, and pathogenesis [PDF]

, 2012
Mycobacterium tuberculosis infection continues to cause substantial human suffering. New chemotherapeutic strategies, which require insight into the pathways essential for M. tuberculosis pathogenesis, are imperative.
Campbell, Elizabeth A., Darst, Seth A., Glickman, Michael S., Harrison, Phillip G., Nickels, Bryce E., Stallings, Christina L., Weiss, Leslie A.   +6 more
core   +2 more sources

Lyophilised Reverse Transcriptase and Polymerase for Point‐of‐Care Diagnostics of SARS‐CoV‐2

Microbial Biotechnology, Volume 18, Issue 7, July 2025.
Design, expression and purification of Klentaq polymerase, Taq polymerase and MMLV reverse transcriptase to prepare a custom‐made amplification mix. To enable cold‐free storage, the amplification mix was lyophilised. The prepared lyophilised amplification mix was used on a point of care device to efficiently detect SARS‐CoV‐2 RNA.
Natalie Mutter, Barbara Posch, Yasmin Gillitschka, Nadezhda Kataeva, Birgit Michalek, Daniel Lager, Filip Staniszewski, Alexandra Schilder, Lidiya Osinkina, Maximilian Westenthanner, Joachim Stehr, Federico Buersgens, Johannes R. Peham   +12 more
wiley   +1 more source