The One Health approach in food safety: Challenges and opportunities
Abstract According to the World Health Organization, contaminated food causes approximately 600 million cases of illness and 420,000 associated deaths worldwide each year. In this scenario, food safety is a crucial task, which is based in the application of correct hygiene practices throughout the entire food production chain: from farm to table ...
Cristina Rodríguez‐Melcón+2 more
wiley +1 more source
In planta imaging of pyridine nucleotides using second‐generation fluorescent protein biosensors
SUMMARY Redox changes of pyridine nucleotides in cellular compartments are highly dynamic and their equilibria are under the influence of various reducing and oxidizing reactions. To obtain spatiotemporal data on pyridine nucleotides in living plant cells, typical biochemical approaches require cell destruction. To date, genetically encoded fluorescent
Shey‐Li Lim+8 more
wiley +1 more source
Serine protease identification (in vitro) and molecular structure predictions (in silico) from a phytopathogenic fungus, Alternaria solani [PDF]
Citation: Chandrasekaran, M., Chandrasekar, R., Sa, T., & Sathiyabama, M. (2014). Serine protease identification (in vitro) and molecular structure predictions (in silico) from a phytopathogenic fungus, Alternaria solani. Retrieved from http://krex.ksu.
Anandan+37 more
core +1 more source
Efficient Interaction between Two GTPases Allows the Chloroplast SRP Pathway to Bypass the Requirement for an SRP RNA [PDF]
Cotranslational protein targeting to membranes is regulated by two GTPases in the signal recognition particle (SRP) and the SRP receptor; association between the two GTPases is slow and is accelerated 400-fold by the SRP RNA.
Chandrasekar, Sowmya+2 more
core +3 more sources
Occurrence of phosphenolpyruvate carboxylase in the extremely thermophilic bacterium Thermus aquaticus [PDF]
In the extreme thermophile Thermus aquaticus, phosphoenolpyruvate carboxylase catalyzes carbon dioxide fixation on the C3 metabolite phosphoenolpyruvate, producing oxaloacetate. In a moderately thermophilic Bacillus species this function is fulfilled by pyruvate carboyxlase.
T K Sundaram, G P Bridger
openaire +3 more sources
Capillary electrophoresis (CE) is a powerful separation technique offering quick and efficient analyses in various fields of bioanalytical chemistry. It is characterized by many well‐known advantages, but one, which is perhaps the most important for this application field, is somewhat overlooked.
Taťána Bržezická+2 more
wiley +1 more source
Contribution of vesicle-protected extracellular DNA to horizontal gene transfer in Thermus spp. [PDF]
Highly efficient apparatus for natural competence and conjugation have been shown as the major contributors to horizontal gene transfer (HGT) in Thermus thermophilus.
Alba Blesa, José Berenguer
core +1 more source
What is the role of microbial biotechnology and genetic engineering in medicine?
Microbial biotechnology, the technological application of microorganisms, has been instrumental in producing significant natural bioactive products. These include antibiotics, antifungals, anticancer drugs, antiparasitics, antivirals, immunosuppressants, toxoid vaccines, and therapeutic enzymes.
Fernando Santos‐Beneit
wiley +1 more source
Structures of an apo and a binary complex of an evolved archeal b family DNA polymerase capable of synthesising highly cy-dye labelled DNA [PDF]
Thermophilic DNA polymerases of the polB family are of great importance in biotechnological applications including high-fidelity PCR. Of particular interest is the relative promiscuity of engineered versions of the exo- form of polymerases from the ...
Holliger, P.+3 more
core +1 more source
Single-molecule microscopy reveals new insights into nucleotide selection by DNA polymerase I. [PDF]
The mechanism by which DNA polymerases achieve their extraordinary accuracy has been intensely studied because of the linkage between this process and mutagenesis and carcinogenesis. Here, we have used single-molecule fluorescence microscopy to study the
Markiewicz, RP+3 more
core +1 more source