Results 71 to 80 of about 24,101 (229)

Solid‐Phase Synthesis and Biological Evaluation of Peptides ADP‐Ribosylated at Histidine

Angewandte Chemie, Volume 136, Issue 4, January 22, 2024.
We report the preparation of ribofuranosylated Fmoc‐histidine building blocks via a Mukaiyama‐type glycosylation. These building blocks were used in solid‐phase peptide synthesis, followed by on‐resin pyrophosphate construction and deprotection to access peptides containing N(τ)‐ and N(π)‐ADP‐ribosylated histidine.
Hugo Minnee, Johannes G. M. Rack, Gijsbert A. van der Marel, Herman S. Overkleeft, Jeroen D. C. Codée, Ivan Ahel, Dmitri V. Filippov   +6 more
wiley   +2 more sources

Accuracy of replication in the polymerase chain reaction. Comparison between Thermotoga maritima DNA polymerase and Thermus aquaticus DNA polymerase

Brazilian Journal of Medical and Biological Research, 1998
For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that
R.S. Diaz, E.C. Sabino
doaj   +1 more source

Caldolysin, a highly active protease from an extremely Thermophilic Bacterium [PDF]

, 1982
Proteases comprise a significant proportion of those proteins which have been subject to detailed characterisation (amino acid sequence and high resolution crystallographic analysis).
Cowan, Don A., Daniel, Roy M., Morgan, Hugh W.   +2 more
core   +1 more source

The taxonomist - an endangered race : a practical proposal for its survival [PDF]

, 2011
Background: Taxonomy or biological systematics is the basic scientific discipline of biology, postulating hypotheses of identity and relationships, on which all other natural sciences dealing with organisms relies.
Haszprunar, Gerhard, Klussmann-Kolb, Annette, Koch, André, Kuhlmann, Michael, Lindberg, David, Wägele, Heike, Wägele, Johann Wolfgang   +6 more
core   +3 more sources

From Metagenomics to Pure Culture: Isolation and Characterization of the Moderately Halophilic Bacterium Spiribacter salinus gen. nov., sp. nov. [PDF]

, 2014
Recent metagenomic studies on saltern ponds with intermediate salinities have determined that their microbial communities are dominated by both Euryarchaeota and halophilic bacteria, with a gammaproteobacterium closely related to the genera ...
Fernández, Ana Beatriz, Ghai, Rohit, León León, María José, Rodríguez Valera, Francisco, Sánchez-Porro Álvarez, Cristina, Ventosa Ucero, Antonio   +5 more
core   +2 more sources

Extremophilic Microorganisms as a Source of Emerging Enzymes for the Food Industry: A Review

Food Science &Nutrition, Volume 13, Issue 1, January 2025.
Enzymes such as amylases, lipases, proteases, xylanases, laccases, pullulanase, chitinases, pectinases, esterases, isomerases, and dehydrogenases could be derived from extremophilic organisms like thermophiles, psychrophiles, acidophiles, alkaliphiles, and halophiles. These enzymes or extremozymes are applicable in the production of safe foods (such as
Tolulope Joshua Ashaolu, Tanu Malik, Rakhi Soni, Miguel A. Prieto, Seid Mahdi Jafari   +4 more
wiley   +1 more source

Contribution of vesicle-protected extracellular DNA to horizontal gene transfer in Thermus spp. [PDF]

, 2016
Highly efficient apparatus for natural competence and conjugation have been shown as the major contributors to horizontal gene transfer (HGT) in Thermus thermophilus.
Alba Blesa, José Berenguer
core   +1 more source

Eine DNA‐Polymerase‐Variante erkennt den epigenetischen Marker 5‐Methylcytosin durch erhöhten Fehleinbau

Angewandte Chemie, Volume 136, Issue 48, November 25, 2024.
Entwicklung einer DNA‐Polymerase aus Thermus aquaticus, die methylierte von unmethylierter DNA durch einen erhöhten Fehleinbau gegenüber 5‐Methylcytosin (5 mC) während der DNA‐Synthese unterscheidet. Durch die Erzeugung von 5 mC‐abhängigen Mutationssignaturen im PCR‐Produkt ist das Enzym in der Lage, methylierte Stellen zu erkennen und ermöglicht den ...
Melanie Henkel, Alexander Fillbrunn, Virginie Marchand, Govindan Raghunathan, Michael R. Berthold, Yuri Motorin, Andreas Marx   +6 more
wiley   +1 more source

A 7-Deazaadenosylaziridine Cofactor for Sequence-Specific Labeling of DNA by the DNA Cytosine-C5 Methyltransferase M.HhaI

Molecules, 2015
DNA methyltransferases (MTases) catalyze the transfer of the activated methyl group of the cofactor S-adenosyl-l-methionine (AdoMet or SAM) to the exocyclic amino groups of adenine or cytosine or the C5 ring atom of cytosine within specific DNA sequences.
Falk Kunkel, Rudi Lurz, Elmar Weinhold
doaj   +1 more source

A DNA Polymerase Variant Senses the Epigenetic Marker 5‐Methylcytosine by Increased Misincorporation

Angewandte Chemie International Edition, Volume 63, Issue 48, November 25, 2024.
Engineering of a Thermus aquaticus DNA polymerase that distinguishes methylated from unmethylated DNA by increased misincorporation opposite 5‐methylcytosine (5 mC) during DNA synthesis. By generating 5 mC‐dependent mutation signatures in the PCR product, the enzyme is able to sense methylated sites and allows direct detection by reading elevated error
Melanie Henkel, Alexander Fillbrunn, Virginie Marchand, Govindan Raghunathan, Michael R. Berthold, Yuri Motorin, Andreas Marx   +6 more
wiley   +1 more source