Results 81 to 90 of about 24,101 (229)
Identification and Characterization of a Thermostable MutS Homolog from Thermus aquaticus [PDF]
Journal of Biological Chemistry, 1996 Recognition of mispaired or unpaired bases during DNA mismatch repair is carried out by the MutS protein family. Here, we describe the isolation and characterization of a thermostable MutS homolog from Thermus aquaticus YT-1. Sequencing of the mutS gene predicts an 89.3-kDa polypeptide sharing extensive amino acid sequence homology with MutS homologs ...
Peggy Hsieh, Indranil Biswas
openaire +3 more sources
The One Health approach in food safety: Challenges and opportunities
Food Frontiers, Volume 5, Issue 5, Page 1837-1865, September 2024.Abstract According to the World Health Organization, contaminated food causes approximately 600 million cases of illness and 420,000 associated deaths worldwide each year. In this scenario, food safety is a crucial task, which is based in the application of correct hygiene practices throughout the entire food production chain: from farm to table ...
Cristina Rodríguez‐Melcón +2 more
wiley +1 more source
Structural and molecular biology of a protein-polymerizing nanomachine for pilus biogenesis [PDF]
, 2017 Bacteria produce protein polymers on their surface called pili or fimbriae that serve either as attachment devices or as conduits for secreted substrates.
Waksman, Gabriel
core +1 more source
In planta imaging of pyridine nucleotides using second‐generation fluorescent protein biosensors
The Plant Journal, Volume 119, Issue 3, Page 1643-1658, August 2024.SUMMARY Redox changes of pyridine nucleotides in cellular compartments are highly dynamic and their equilibria are under the influence of various reducing and oxidizing reactions. To obtain spatiotemporal data on pyridine nucleotides in living plant cells, typical biochemical approaches require cell destruction. To date, genetically encoded fluorescent
Shey‐Li Lim +8 more
wiley +1 more source
Multiple prebiotic metals mediate translation. [PDF]
, 2018 Today, Mg2+ is an essential cofactor with diverse structural and functional roles in life's oldest macromolecular machine, the translation system. We tested whether ancient Earth conditions (low O2, high Fe2+, and high Mn2+) can revert the ribosome to a ...
Bowman, Jessica C +8 more
core +1 more source
Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli [PDF]
Analytical Biochemistry, 1990 DNA polymerase from Thermus aquaticus has become a common reagent in molecular biology because of its utility in DNA amplification and DNA sequencing protocols. A simplified method is described here for isolating the recombinant Taq enzyme after overproduction in Escherichia coli.
Engelke, David R. +3 more
openaire +4 more sources
Response of the thermophile Thermus sp. RQ-1 to hyperbaric air in batch and fed-batch cultivation [PDF]
, 2000 The effects of increased air pressure in a cul- ture of the thermophilic microorganism Thermus sp. RQ-1 were investigated. Cell growth dependence on oxygen supply was investigated in a fermenter at atmo- spheric pressure.
Belo, Isabel, Mota, M., Pinheiro, Rita
core +1 more source
Journal of Separation Science, Volume 47, Issue 13, July 2024.Capillary electrophoresis (CE) is a powerful separation technique offering quick and efficient analyses in various fields of bioanalytical chemistry. It is characterized by many well‐known advantages, but one, which is perhaps the most important for this application field, is somewhat overlooked.
Taťána Bržezická +2 more
wiley +1 more source
What is the role of microbial biotechnology and genetic engineering in medicine?
MicrobiologyOpen, Volume 13, Issue 2, April 2024.Microbial biotechnology, the technological application of microorganisms, has been instrumental in producing significant natural bioactive products. These include antibiotics, antifungals, anticancer drugs, antiparasitics, antivirals, immunosuppressants, toxoid vaccines, and therapeutic enzymes.
Fernando Santos‐Beneit
wiley +1 more source
Reversibly locked thionucleobase pairs in DNA to study base flipping enzymes
Beilstein Journal of Organic Chemistry, 2014 Covalently interstrand cross-linked DNA is an interesting tool to study DNA binding proteins that locally open up the DNA duplex by flipping single bases out of the DNA helix or melting whole stretches of base pairs to perform their function.
Christine Beuck, Elmar Weinhold
doaj +1 more source