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A DING phosphatase in Thermus thermophilus
Amino Acids, 2007Phosphate transport in bacteria occurs via a phosphate specific transporter system (PSTS) that belongs to the ABC family of transporters, a multisubunit system, containing an alkaline phosphatase. DING proteins were characterized due to the N-terminal amino acid sequence DINGG GATL, which is highly conserved in animal and plant isolates, but more ...
Pantazaki, A. A. +2 more
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Biotechnologically relevant enzymes from Thermus thermophilus
Applied Microbiology and Biotechnology, 2002Enzymes produced by Thermus thermophilus are of considerable biotechnological interest. This review covers industrial applications of several protein products of this thermophilic bacterium that are functional under extreme conditions. The purification of proteins from T.
A A, Pantazaki +2 more
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Thermus thermophilus ribosomes for crystallographic studies
Biochimie, 1991Three-dimensional crystals of the 70S ribosomes, the 70S ribosome-mRNA-tRNA complex, the 30S ribosomal subunits, several ribosomal proteins, the elongation factor G and threonyl- and seryl-tRNA synthetases from a Gram-negative extreme thermophilic bacterium, Thermus thermophilus, have been obtained at our institute.
M M, Yusupov +3 more
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Growth kinetics of Thermus thermophilus
European Journal of Applied Microbiology and Biotechnology, 1982The nonsporulating extreme thermophile Thermus thermophilus was grown in continuous culture at dilution rates up to 2.65 h−1 at 75°C and pH 6.9 on complex medium. Concomitantly very low yield (Y=0.12 g cell dry weight g−1 utilized organic carbon) and incomplete substrate utilization (always less than 45%) were found. In batch cultures T.
B. Sonnleitner, S. Cometta, A. Fiechter
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������������ ������ ���������������� ������ PHA ���������������� ������ Thermus thermophilus:
2008Polyhydroxyalkanoic acids (PHAs) are carbon and energy storage polymers synthesized by most embecteria and archea in response to environmental conditions, such as an excess of carbon source and a limitation of the other nutrients. PHA granules are surrounded by a phospholipid membrane with embedded, or attached proteins such as PHA synthese, PHA ...
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A DNA Methylase from Thermus thermophilus HB8
The Journal of Biochemistry, 1980A DNA methylase was purified in a homogeneous state from a extremely thermophilic bacterium, Thermus thermophilus HB8, by chromatography on, successively, phosphocellulose, CM-cellulose, and heparin-Sepharose. The molecular weight of the enzyme was determined to be about 44,000 by gel filtration on a Sephadex G-100 column and 41,000 by SDS-poly ...
S, Sato, K, Nakazawa, T, Shinomiya
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2011
Firstly, the bacterial strain hermus thermophilus HB8 was grown in a medium containing as the sole carbon source sodium alginate 0.2 % w/v and the extracellular medium was isolated. Then the extracellular medium was lyophilized to a final volume of 20 ml and siderophores were determined using the specific CAS solution (chromoazurol).
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Firstly, the bacterial strain hermus thermophilus HB8 was grown in a medium containing as the sole carbon source sodium alginate 0.2 % w/v and the extracellular medium was isolated. Then the extracellular medium was lyophilized to a final volume of 20 ml and siderophores were determined using the specific CAS solution (chromoazurol).
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