Results 41 to 50 of about 309,365 (304)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Study The Chemical Analysis and Lethal dose of Alcoholic Extract For Qunabrey Plant Cardaria Draba(L.)Desv. [PDF]

open access: yesKirkuk Journal of Science, 2018
This study was aimed to obtain the alcoholic extract of cardaria draba (L.) Des .plant of ethanol %70,%96 in both dry and fresh parts respectively. The weight of the extract of the c.draba plant was 55.70,8.71 for dry part and fresh part respectively ...
Sadia hamad   +2 more
doaj   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

open access: yesFEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth   +6 more
wiley   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

open access: yesFEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee   +6 more
wiley   +1 more source

Oxalate-silica gel thin-layer system for free 2-hydroxy fatty acids and for fatty acyl coenzyme A

open access: yesJournal of Lipid Research, 1972
The 2-hydroxy fatty acids tend to yield streaks in thin-layer chromatography on silica gel plates. If potassium oxalate is included with binder-free silica gel, good spots are obtained.
M. David Ullman, Norman S. Radin
doaj   +1 more source

Preliminary qualitative screening for cancer chemopreventive agents in Telfairia occidentalis Hook.f., Gnetum africanum Welw., Gongronema latifolium Benth. and Ocimum gratissimum L. from Nigeria [PDF]

open access: yes, 2009
The leaves of four plant foods commonly consumed in Nigeria namely Gnetum africanum (Igbo name: Ukazi), Gongronema latifolium (Igbo name: Utazi), Telfairia occidentalis (Igbo name: Ugu), Ocimum gratissimum (Igbo name: Nchoanwu), were each screened for ...
Iweala, E. E. J.
core  

Performance of Electropun Polyacrylonitrile Nanofibrous Phases, Shown for the Separation of Water-Soluble Food Dyes via UTLC-Vis-ESI-MS [PDF]

open access: yes, 2006
Research in the miniaturization of planar chromatography led to various approaches in manufacturing ultrathin-layer chromatography (UTLC) layers of reduced thickness (
Andrew, Lachlan L. H.   +3 more
core   +2 more sources

Multimodal Soft Surgical Robots Enabled by Eco‐Degradable, Sterilizable Polymers and Transient Electronics

open access: yesAdvanced Functional Materials, EarlyView.
A compostable PGS soft surgical robot with interchangeable modules integrates transient Mo tactile and Si thermal sensors for dual feedback. The device preserves its function after clinical‐grade sterilization, demonstrates stable actuation and cardiac tissue grasping with real‐time in vivo pulsatile monitoring, and biodegrades post‐use with soil‐safe, 
Minseong Chae   +27 more
wiley   +1 more source

Distribution of fatty acids in triacylglycerols from ungrafted Desi Mango (Mangifera indica) kernel lipids

open access: yesJournal of Saudi Chemical Society, 2010
The lipids of ungrafted ‘Desi’ mango (Mangifera indica) kernel were extracted with chloroform/methanol, purified and triacylglycerols (82.6%) were separated by thin layer chromatography (TLC). The pure triglycerides are fractionated into five bands by 25%
Zeeshan Ali   +3 more
doaj   +1 more source

Comparative chromatography of chloroplast pigment [PDF]

open access: yes, 1969
Methods for isolation of low concentration pigments of the cocklebur species are described. The methods entail two step chromatography so that the different sorption properties of the various pigments in varying column parameters can be utilized ...
Grandolfo, M., Sherma, J., Strain, H. H.
core   +1 more source

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