Results 41 to 50 of about 265,797 (310)
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
The (small) vibrations of thin plates* [PDF]
Nonlinearity, 2019 Abstract We describe the equations of motion of elastodynamic bounded bodies in 3-space, and their linearizations at a stationary point. Using the latter as an approximation to model small motions, we develop a scheme to find numerical solutions of these equations. We discretize the solution in the space of PL vector fields associated
openaire +2 more sources
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains
FEBS Open Bio, EarlyView.The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth +6 more
wiley +1 more source
The Dynamic Response and Failure Model of Thin Plate Rock Mass under Impact Load
Shock and Vibration, 2021 The layered rock mass widely exists in mining, construction, transportation, and water conservancy projects, and the damage phenomena of plate crack and spalling often occurs in the process of coal and rock dynamic disaster in deep mining. Therefore, the
Feng Li +5 more
doaj +1 more source
FEBS Open Bio, EarlyView.This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
Drying-Induced Strain-Stress and Deformation of Thin Ceramic Plate
ChemEngineering, 2020 Ceramic thin plates are applied to several industrial purposes including electronic materials and sensors. Drying-induced shrinkage and strain-stress formation of a ceramic thin plate were studied experimentally and theoretically.
Yoshinori Itaya +3 more
doaj +1 more source
, 2011 Copyright @ 2011 Sage Publications LtdSmart structures are components used in engineering applications that are capable of sensing or reacting to their environment in a predictable and desired manner.
Lawrie, Jane B. +5 more
core +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source