Results 151 to 160 of about 1,526,370 (261)

Wan qiu ti ba

open access: yes
V.1-3. 西山題跋 -- v.4. 无咎題跋 -- v.5. 淮海題跋 -- v.6. 宛丘題跋.V.1-3. Xi shan ti ba -- v.4. Wu jiu ti ba -- v.5. Huai hai ti ba -- v.6. Wan qiu ti ba.眞德秀撰 ; 毛晋訂. 无咎題跋 / 晁補之撰 ; 毛晋訂. 淮海題跋 / 秦觀撰 ; 毛晋訂. 宛丘題跋 / 張耒撰 ; 毛晋訂.綫裝.框18.9x13.5公分, 8行19字. 白口, 左右雙邊, 無魚尾. 版心上鐫題名,

core  

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

open access: yesFEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli   +3 more
wiley   +1 more source

Structures of Ti(IV)-doped alpha-FeOOH particles

open access: yes
The influence of Ti(IV) on the formation and structure of alpha-FeOOH particles was investigated by characterizing the coprecipitation products of Fe(III) with Ti(IV) at different atomic ratios (Ti/Fe) in the range 0-0.1.
神鳥, 和彦   +14 more
core  

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty   +13 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

open access: yesFEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Heterotropic regulation and negative homotropic cooperativity

open access: yesFEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea   +5 more
wiley   +1 more source

Electrosprayed Chitosan-Calcium Phosphate Microshell Composite Coatings on Silanized Titanium Substrates. [PDF]

open access: yesJ Funct Biomater
Watson AB   +5 more
europepmc   +1 more source

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