Results 41 to 50 of about 1,097,268 (266)

Time-resolved burst variance analysis

open access: yesBiophysical Reports, 2023
Quantifying biomolecular dynamics has become a major task of single-molecule fluorescence spectroscopy methods. In single-molecule Förster resonance energy transfer (smFRET), kinetic information is extracted from the stream of photons emitted by attached
Ivan Terterov   +3 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

open access: yesFEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho   +3 more
wiley   +1 more source

Time-resolved scRNA-seq reveals transcription dynamics of polarized macrophages with influenza A virus infection and antigen presentation to T cells

open access: yesEmerging Microbes and Infections
Throughout history, the influenza A virus has caused numerous devastating global pandemics. Macrophages, as pivotal innate immune cells, exhibit a wide range of immune functions characterized by distinct polarization states, reflecting their intricate ...
Jiapei Yu   +13 more
doaj   +1 more source

Time resolved femtosecond spectroscopy of nanocomposites

open access: yesEPJ Web of Conferences, 2018
Characterization methods of nanocomposites consisted of semiconductor metal-oxide quantum dots (QD) incorporated into a dielectric matrix have been elaborated on the base of time resolved four-wave mixing and photon echo.
Khasanov Oleg   +5 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

open access: yesFEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe   +3 more
wiley   +1 more source

Time resolved 3D live-cell imaging on implants. [PDF]

open access: yesPLoS ONE, 2018
It is estimated that two million new dental implants are inserted worldwide each year. Innovative implant materials are developed in order to minimize the risk of peri-implant inflammations. The broad range of material testing is conducted using standard
Alexandra Ingendoh-Tsakmakidis   +8 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

open access: yesFEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes   +4 more
wiley   +1 more source

Time-Resolved Measurement of a Charge Qubit [PDF]

open access: yesPhysical Review Letters, 2009
We propose a scheme for monitoring coherent quantum dynamics with good time-resolution and low backaction, which relies on the response of the considered quantum system to high-frequency ac driving. An approximate analytical solution of the corresponding quantum master equation reveals that the phase of an outgoing signal, which can directly be ...
Reuther, Georg M.   +3 more
openaire   +4 more sources

Magnetron-Sputtered Polytetrafluoroethylene-Stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence

open access: yesNanomaterials, 2020
Surface-enhanced fluorescence (SEF) requires the absorption/emission band of the fluorophore, the localized surface plasmon resonance (LSPR) of the nanostructure and the excitation wavelength to fall in the same (or very close) spectral range.
Martin Šubr   +7 more
doaj   +1 more source

Time Resolved Reflectivity Measurements of Convective Clouds

open access: yesGeophysical Research Letters, 2023
National Aeronautics and Space Administration's Investigations of Convective Updrafts (INCUS) mission aims to document convective mass flux through changes in the radar reflectivity (ΔZ) in convective cores captured by a constellation of three Ka‐band ...
Brenda Dolan   +10 more
doaj   +1 more source

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