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Time-resolved macromolecular crystallography
Current Opinion in Structural Biology, 1992Abstract The number of research projects in the field of time-resolved macromolecular crystallography is still very limited. Although there has been constant progress in monitoring enzymatic reactions in crystals by applying diffration methods to determine the accompanying structural changes, it is still mostly the suitability of the system that ...
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Time-resolved crystallography on H-ras p21
Philosophical Transactions of the Royal Society of London. Series A: Physical and Engineering Sciences, 1992Abstract We describe here the results obtained to date on a project aimed at characterizing the changes occurring in the protein product (p21) of the H-ras proto-oncogene during and as a result of hydrolysis of GTP at its active site.
Axel J. Scheidig +6 more
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Time-resolved Macromolecular Crystallography Workshop at BioCARS
Synchrotron Radiation News, 2009On November 20–22, 2008, BioCARS, a member of the Center for Advanced Radiation Sources at The University of Chicago, hosted a three-day workshop on Time-resolved Macromolecular Crystallography (TRMC) that introduced participants to the principles of synchrotron-based Laue diffraction for time-resolved crystallography and provided hands-on instruction ...
Jane Andrew +4 more
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The Laue Method and its Use in Time-Resolved Crystallography
1989A review is given of the Laue method and its uses in time-resolved crystallography. The history of the method is covered and various fundamental problems, as outlined by W. H. and W. L. Bragg and R. W. G. Wyckoff, are dealt with. The details of the diffraction geometry, wavelength normalization, and energy deconvolution are given. A survey is then made
Keith Moffat, John R. Helliwell
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Time-resolved crystallography: principles, problems and practice
Philosophical Transactions of the Royal Society of London. Series A: Physical and Engineering Sciences, 1992Abstract Time-resolved crystallography is founded on the belief that a complete understanding of mechanism at the molecular level demands knowledge, not just of long-lived, readily observable structures, but also of short-lived intermediates in processes such as catalysis, ligand binding and release, and protein unfolding. Synchrotron
Henry Keith Moffatt +4 more
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Protein-Ligand Interaction Probed by Time-Resolved Crystallography
2005Time-resolved (TR) crystallography is a unique method for determining the structures of intermediates in biomolecular reactions. The technique reached its mature stage with the development of the powerful third-generation synchrotron X-ray sources, and the advances in data processing and analysis of time-resolved Laue crystallographic data.
Schmidt, M. +3 more
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Reactions in crystals and time-resolved crystallography
2000Abstract In the general excitement of a time when new protein structures are published every week, it is often forgotten that a structure in itself does not tell one how an enzyme works. For that, one needs to know a great deal about mechanism, intermediates,1 and dynamics; a structure is only a beginning, although an important one.
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Time-resolved Crystallography on Protein Photoreceptors and Enzymes
2023Time-resolved macromolecular crystallography (TRX) unifies structure determination of biomolecules with chemical kinetics. Developed at synchrotron light sources, TRX has been transformed by serial femtosecond crystallography (SFX) at X-ray free electron laser (XFEL) facilities.
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Overview of time-resolved macromolecular crystallography
1997Abstract Time-resolved macromolecular crystallography is a new capability, but has been of interest for some years. It has been driven so far by new technologies and methods involving synchrotron radiation (SR) sources, optics, and detectors. Additionally, the initiation and monitoring of reactions in crystals is relatively new (see e.
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Laue crystallography: time-resolved studies
2006The term ‘Laue diffraction’ describes the process of X-ray scattering that occurs when a stationary crystal is illuminated by a polychromatic beam of X-rays. Recent developments in Laue diffraction have depended on three main advances: the use of very intense polychromatic synchrotron sources; the realization that the so-called energy-overlap or ...
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